Venkata Lakshmi Manasa Chanduri
Associate Research ScientistCards
About
Research
Publications
2024
Cellular stiffness sensing through talin 1 in tissue mechanical homeostasis
Chanduri M, Kumar A, Weiss D, Emuna N, Barsukov I, Shi M, Tanaka K, Wang X, Datye A, Kanyo J, Collin F, Lam T, Schwarz U, Bai S, Nottoli T, Goult B, Humphrey J, Schwartz M. Cellular stiffness sensing through talin 1 in tissue mechanical homeostasis. Science Advances 2024, 10: eadi6286. PMID: 39167642, PMCID: PMC11338229, DOI: 10.1126/sciadv.adi6286.Peer-Reviewed Original ResearchConceptsTissue mechanical homeostasisStiffness sensingExtracellular matrixTalin-1Mechanical homeostasisExtracellular matrix mechanicsIncreased cell spreadingCell spreadingTalinMutationsCellular sensingFibrillar collagenReduced axial stiffnessTissue mechanical propertiesMechanical propertiesAxial stiffnessCompliant substratesHomeostasisRupture pressureArp2/3ARPC5LStiffnessHomeostasis hypothesisResident cellsTissue stiffnessMechanosensing through talin 1 contributes to tissue mechanical homeostasis.
Chanduri MVL, Kumar A, Weiss D, Emuna N, Barsukov I, Shi M, Tanaka K, Wang X, Datye A, Kanyo J, Collin F, Lam T, Schwarz UD, Bai S, Nottoli T, Goult BT, Humphrey JD, Schwartz MA. Mechanosensing through talin 1 contributes to tissue mechanical homeostasis. BioRxiv 2024 PMID: 38328095, DOI: 10.1101/2023.09.03.556084.Peer-Reviewed Original Research In Press
2023
TLN1 contains a cancer-associated cassette exon that alters talin-1 mechanosensitivity
Gallego-Paez L, Edwards W, Chanduri M, Guo Y, Koorman T, Lee C, Grexa N, Derksen P, Yan J, Schwartz M, Mauer J, Goult B. TLN1 contains a cancer-associated cassette exon that alters talin-1 mechanosensitivity. Journal Of Cell Biology 2023, 222: e202209010. PMID: 36880935, PMCID: PMC9997659, DOI: 10.1083/jcb.202209010.Peer-Reviewed Original ResearchConceptsExon 17bTerminal FERM domainVinculin bindingFERM domainSwitch domainAdhesion dynamicsCassette exonsSplicing analysisAdapter proteinTLN1Single isoformIsoform switchTalin-1Amino acidsFrame insertionExonsBiochemical analysisIsoformsProteinExon 17CytoskeletonGenesMechanotransductionDomainIntegrins
2019
Back-Pyrophosphorylation Assay to Detect In Vivo InsP7-Dependent Protein Pyrophosphorylation in Mammalian Cells
Chanduri M, Bhandari R. Back-Pyrophosphorylation Assay to Detect In Vivo InsP7-Dependent Protein Pyrophosphorylation in Mammalian Cells. Methods In Molecular Biology 2019, 2091: 93-105. PMID: 31773573, DOI: 10.1007/978-1-0716-0167-9_8.Peer-Reviewed Original ResearchConceptsTarget proteinsProtein pyrophosphorylationAmount of radiolabelled phosphateWild-type cellsPhosphorylated serine residuesInositol pyrophosphatesPosttranslational modificationsSerine residuesMammalian cellsDiphosphoinositol pentakisphosphateInsP7Endogenous proteinsNative proteinPyrophosphorylationPhysiological processesProteinCell typesCellsAssayPentakisphosphateResiduesTargetInositolPyrophosphate
2016
Inositol hexakisphosphate kinase 1 (IP6K1) activity is required for cytoplasmic dynein-driven transport
Chanduri M, Rai A, Malla A, Wu M, Fiedler D, Mallik R, Bhandari R. Inositol hexakisphosphate kinase 1 (IP6K1) activity is required for cytoplasmic dynein-driven transport. Biochemical Journal 2016, 473: 3031-3047. PMID: 27474409, PMCID: PMC5095903, DOI: 10.1042/bcj20160610.Peer-Reviewed Original ResearchConceptsInositol pyrophosphatesP150(Glued) subunit of dynactinIntermediate chainEukaryotic signaling moleculesRecruitment to membranesDynein intermediate chainP150(Glued) subunitSubunit of dynactinInositol hexakisphosphate kinase 1Inositol hexakisphosphate kinasesSerine pyrophosphorylationEndosomal sortingGolgi maintenanceVesicle transportTrafficking pathwaysVesicle movementDynein functionProtein functionMammalian cellsDiphosphoinositol pentakisphosphateDisplay defectsN-terminusRegulatory signalsMonophosphate moietyMicrotubule transport
2012
Inositol Pyrophosphate Synthesis by Inositol Hexakisphosphate Kinase 1 Is Required for Homologous Recombination Repair*
Jadav R, Chanduri M, Sengupta S, Bhandari R. Inositol Pyrophosphate Synthesis by Inositol Hexakisphosphate Kinase 1 Is Required for Homologous Recombination Repair*. Journal Of Biological Chemistry 2012, 288: 3312-3321. PMID: 23255604, PMCID: PMC3561551, DOI: 10.1074/jbc.m112.396556.Peer-Reviewed Original ResearchConceptsMouse embryonic fibroblastsKnock-out mouse embryonic fibroblastsInositol pyrophosphate synthesisInositol hexakisphosphate kinase 1Inositol pyrophosphatesHomologous recombinationPyrophosphate synthesisKinase 1DNA damage sitesDNA damageHR-mediated repairDNA damage repairSmall molecule regulatorsImpaired DNA damage repairInduction of DNA damageGenotoxic stressWater-soluble inositol phosphatesDiphosphate moietyDiphosphoinositol pentakisphosphateInositol ringNuclear fociDNA repairHR repairEmbryonic fibroblastsIP6K1