Alice Sherrard
Associate Research Scientist in GeneticsCards
About
Research
Publications
2025
ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms
Young L, Sherrard A, Zhou H, Shaikh F, Hutchings J, Riggi M, Narasimhan M, Flaherty W, Bennett E, Rosen M, Giraldez A, Villa E. ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms. Nature Methods 2025, 23: 131-142. PMID: 41315814, PMCID: PMC12791015, DOI: 10.1038/s41592-025-02928-4.Peer-Reviewed Original ResearchCryo-ETIn situ cryo-electron microscopyNanogold probeLiving cellsCryo-electron tomogramsCryo-EMCryo-electron tomographyCryo-EM dataLow copy numberNative cellular environmentCryo-electron microscopyStructure-function relationshipsNuclear proteinsCopy numberNanogold labelingSize scalesIdentification of macromoleculesCryo-electronProtein labelingMolecular networksCellular environmentProteinCorrelative lightCell viabilityElectron microscopyGenetically encoded affinity reagents are a toolkit for visualizing and manipulating endogenous protein function in vivo
Boswell C, Hoppe C, Sherrard A, Miao L, Kojima M, Martino P, Zhao N, Stasevich T, Nicoli S, Giraldez A. Genetically encoded affinity reagents are a toolkit for visualizing and manipulating endogenous protein function in vivo. Nature Communications 2025, 16: 5503. PMID: 40595661, PMCID: PMC12216260, DOI: 10.1038/s41467-025-61003-w.Peer-Reviewed Original ResearchConceptsFunction in vivoAffinity reagentsPlanar cell polarity protein Vangl2Gene targetingEndogenous protein localizationProtein function in vivoProtein targets in vivoModel organism communitiesKnock-in alleleConventional gene targetingProtein functionSingle chain variable fragmentTranscription factor NanogProtein localizationTargets in vivoZebrafish developmentVariable fragmentAllelesNative behaviorFluorescence visualizationOrganic communityVangl2AffinityGenesZebrafish
2023
Chromatin expansion microscopy reveals nanoscale organization of transcription and chromatin
Pownall M, Miao L, Vejnar C, M'Saad O, Sherrard A, Frederick M, Benitez M, Boswell C, Zaret K, Bewersdorf J, Giraldez A. Chromatin expansion microscopy reveals nanoscale organization of transcription and chromatin. Science 2023, 381: 92-100. PMID: 37410825, PMCID: PMC10372697, DOI: 10.1126/science.ade5308.Peer-Reviewed Original ResearchConceptsZygotic genome activationTranscriptional elongationExpansion microscopyRNA polymerase IIChromatin regulatory factorsEnhancer-promoter contactsGenome activationChromatin organizationNuclear organizationPolymerase IIPol IIFactor NanogTranscription factorsGene expressionRegulatory factorsChromatinNanoscale organizationNanogTranscriptionElongationNucleosomesUniversal processPromoterEmbryosEnhancerCTLA4 depletes T cell endogenous and trogocytosed B7 ligands via cis-endocytosis
Xu X, Dennett P, Zhang J, Sherrard A, Zhao Y, Masubuchi T, Bui J, Chen X, Hui E. CTLA4 depletes T cell endogenous and trogocytosed B7 ligands via cis-endocytosis. Journal Of Experimental Medicine 2023, 220: e20221391. PMID: 37042938, PMCID: PMC10103642, DOI: 10.1084/jem.20221391.Peer-Reviewed Original ResearchConceptsT cellsMajor histocompatibility complexB7 ligandsHuman regulatory T cellsRegulatory T cellsSurface of T cellsCD28 costimulatory signalsAntigen-presenting-cellsAdaptive immune responsesHuman T cellsT cell coreceptorsCostimulatory signalsLigands CD80CTLA4Cell-intrinsicImmune responseCD28Histocompatibility complexTrogocytosisCD28:B7TregsCD80CD86Extrinsic functionsBlockade