Xin Li
Associate Research ScientistCards
About
Research
Publications
2025
Advanced tissue-engineered pulsatile conduit using human induced pluripotent stem cell-derived cardiomyocytes
Luo H, Anderson C, Li X, Lu Y, Hoareau M, Xing Q, Fooladi S, Liu Y, Xu Z, Park J, Fallon M, Thomas J, Gruber P, Elder R, Mak M, Riaz M, Campbell S, Qyang Y. Advanced tissue-engineered pulsatile conduit using human induced pluripotent stem cell-derived cardiomyocytes. Acta Biomaterialia 2025 PMID: 40582540, PMCID: PMC12338880, DOI: 10.1016/j.actbio.2025.06.055.Peer-Reviewed Original ResearchSingle ventricle congenital heart defectsHuman induced pluripotent stem cell-derived cardiomyocytesPluripotent stem cell-derived cardiomyocytesStem cell-derived cardiomyocytesCell-derived cardiomyocytesCongenital heart defectsHuman umbilical arteryUmbilical arteryHeart defectsPulmonary circulationDecellularized human umbilical arteriesHeart tissueLife-threatening defectsLife-threatening disorderLong-term complicationsEngineered Heart TissueFontan surgeryFunctioning ventriclePrompt treatmentHeart failureSpontaneous beatingPump functionImprove outcomesPressure generationConventional treatmentUnveiling a novel cancer hallmark by evaluation of neural infiltration in cancer
Dong Q, Guo Y, Lv C, Ren L, Chen B, Wang Y, Liu Y, Liu M, Liu K, Zhang N, Wang L, Sang S, Li X, Hui Y, Liang H, Gu Y. Unveiling a novel cancer hallmark by evaluation of neural infiltration in cancer. Briefings In Bioinformatics 2025, 26: bbaf082. PMID: 40052442, PMCID: PMC11886572, DOI: 10.1093/bib/bbaf082.Peer-Reviewed Original ResearchConceptsCancer hallmarksSingle-cell RNA sequencing datasetsRNA-seq datasetsTumor microenvironmentNeural infiltrationCopy number variationsRNA sequencing datasetsEpithelial cellsRNA-seqSequencing datasetsNumber variationsEpithelial-mesenchymal transition scoresCancer typesMultiple cancer typesAntitumor immune cellsNeural genesSchwann cellsSignaling pathwayCell differentiationEffective therapeutic strategyCancer cellsCell typesCo-culture experimentsCancer samplesCancer progression
2024
Vascular endothelial cells derived from transgene-free pig induced pluripotent stem cells for vascular tissue engineering
Batty L, Park J, Qin L, Riaz M, Lin Y, Xu Z, Gao X, Li X, Lopez C, Zhang W, Hoareau M, Fallon M, Huang Y, Luo H, Luo J, Ménoret S, Li P, Jiang Z, Smith P, Sachs D, Tellides G, Anegon I, Pober J, Liu P, Qyang Y. Vascular endothelial cells derived from transgene-free pig induced pluripotent stem cells for vascular tissue engineering. Acta Biomaterialia 2024, 193: 171-184. PMID: 39681154, PMCID: PMC12212065, DOI: 10.1016/j.actbio.2024.12.033.Peer-Reviewed Original ResearchInduced pluripotent stem cellsVascular tissue engineeringPig induced pluripotent stem cellsPluripotent stem cellsEndothelial cellsLarge animal modelStem cellsAnimal modelsTissue engineeringInferior vena cava graftHuman induced pluripotent stem cellsEffective differentiation protocolsPreclinical large animal modelExpression of endothelial markersCell-based therapiesExtensive preclinical testingPig endothelial cellsFunctional endothelial cellsIn vivo functional studiesTreatment of cardiovascular diseasesVascular endothelial cellsTissue engineering therapiesTransplant therapeuticsEfficacy of tissueImmunodeficient ratsFully biologic endothelialized-tissue-engineered vascular conduits provide antithrombotic function and graft patency
Park J, Riaz M, Qin L, Zhang W, Batty L, Fooladi S, Kural M, Li X, Luo H, Xu Z, Wang J, Banno K, Gu S, Yuan Y, Anderson C, Ellis M, Zhou J, Luo J, Shi X, Shin J, Liu Y, Lee S, Yoder M, Elder R, Mak M, Thorn S, Sinusas A, Gruber P, Hwa J, Tellides G, Niklason L, Qyang Y. Fully biologic endothelialized-tissue-engineered vascular conduits provide antithrombotic function and graft patency. Cell Stem Cell 2024, 32: 137-143.e6. PMID: 39644899, PMCID: PMC11698629, DOI: 10.1016/j.stem.2024.11.006.Peer-Reviewed Original ResearchTissue-engineered vascular conduitsSingle-ventricle congenital heart defectsEndothelial cellsBiodegradable polymeric scaffoldsGraft patencyAutologous bone marrow cellsAntithrombotic functionCongenital heart defectsInferior vena cava graftHiPSC-derived endothelial cellsBone marrow cellsHuman umbilical arteryDecellularized human umbilical arteriesPolymeric scaffoldsHost endothelial cellsHuman induced pluripotent stem cell (hiPSC)-derived endothelial cellsUmbilical arteryHeart defectsVascular conduitsMarrow cellsFlow bioreactorVena cava graftNude ratsGraft stenosisClinical trialsUBXN9 governs GLUT4-mediated spatial confinement of RIG-I-like receptors and signaling
Harrison A, Yang D, Cahoon J, Geng T, Cao Z, Karginov T, Hu Y, Li X, Chiari C, Qyang Y, Vella A, Fan Z, Vanaja S, Rathinam V, Witczak C, Bogan J, Wang P. UBXN9 governs GLUT4-mediated spatial confinement of RIG-I-like receptors and signaling. Nature Immunology 2024, 25: 2234-2246. PMID: 39567760, PMCID: PMC12067455, DOI: 10.1038/s41590-024-02004-7.Peer-Reviewed Original ResearchConceptsRIG-I-like receptorsRIG-I-like receptor signalingCytosolic RIG-I-like receptorsAntiviral immunityPlasma membrane tetheringRNA virus infectionGlucose transportInnate antiviral immunityCytoplasmic RIG-I-like receptorsGolgi matrixGLUT4 translocationRLR signalingViral RNACell surfaceGLUT4GLUT4 expressionGlucose uptakeInterferon responseRNAGlycolytic reprogrammingVirus infectionHuman inflammatory myopathiesGolgiSignalUbiquitinDe Novo Elastin Assembly Alleviates Development of Supravalvular Aortic Stenosis—Brief Report
Ellis M, Riaz M, Huang Y, Anderson C, Hoareau M, Li X, Luo H, Lee S, Park J, Luo J, Batty L, Huang Q, Lopez C, Reinhardt D, Tellides G, Qyang Y. De Novo Elastin Assembly Alleviates Development of Supravalvular Aortic Stenosis—Brief Report. Arteriosclerosis Thrombosis And Vascular Biology 2024, 44: 1674-1682. PMID: 38752350, PMCID: PMC11209776, DOI: 10.1161/atvbaha.124.320790.Peer-Reviewed Original ResearchSupravalvular aortic stenosisVascular smooth muscle cellsSmooth muscle cellsMuscle cellsAortic stenosisMedial vascular smooth muscle cellsVascular proliferative diseasesEpigallocatechin gallate treatmentProliferative abnormalitiesPreclinical findingsHeart failureLuminal occlusionMouse modelCell hyperproliferationDefective elastinProliferative diseasesCardiovascular disordersFormation of elastinTherapeutic interventionsElastin assemblyElastin depositionStenosisMiceAortic mechanicsImproper formation
2021
Fibroblast Growth Factor 21 Ameliorates NaV1.5 and Kir2.1 Channel Dysregulation in Human AC16 Cardiomyocytes
Li J, Li Y, Liu Y, Yu H, Xu N, Huang D, Xue Y, Li S, Chen H, Liu J, Li Q, Zhao Y, Zhang R, Xue H, Sun Y, Li M, Li P, Liu M, Zhang Z, Li X, Du W, Wang N, Yang B. Fibroblast Growth Factor 21 Ameliorates NaV1.5 and Kir2.1 Channel Dysregulation in Human AC16 Cardiomyocytes. Frontiers In Pharmacology 2021, 12: 715466. PMID: 34630093, PMCID: PMC8493335, DOI: 10.3389/fphar.2021.715466.Peer-Reviewed Original ResearchAction potential durationVentricular arrhythmiasMyocardial infarctionMI ratsDay 1Pharmacologic effects of FGF21Saline-treated MI ratsElectrophysiological functionDay 7Cardiac sodium currentEffects of FGF21Pharmacological effectsArrhythmia modelInwardly rectifying potassiumLethal ventricular arrhythmiasAC16 cellsFGF receptorsOuabain modelRegulation of FGF21Antiarrhythmic therapyFibroblast growth factor 21Sodium currentSerum levelsPotential durationRectifying potassiumUpregulation of transient receptor potential melastatin 4 (TRPM4) in ventricular fibroblasts from heart failure patients
Feng J, Zong P, Yan J, Yue Z, Li X, Smith C, Ai X, Yue L. Upregulation of transient receptor potential melastatin 4 (TRPM4) in ventricular fibroblasts from heart failure patients. Pflügers Archiv - European Journal Of Physiology 2021, 473: 521-531. PMID: 33594499, PMCID: PMC8857941, DOI: 10.1007/s00424-021-02525-2.Peer-Reviewed Original ResearchConceptsTransient receptor potential melastatin 4Heart failure patientsControl individualsCardiac conductionTRPM4 activityFailure patientsNonselective monovalent cation channelImpaired cardiac conductionIntracellular calcium activityTRP channel superfamilyMonovalent cation channelLoss-of-function mutationsCardiac myocytesVentricular fibroblastsCation channelsConduction defectsHeart failureCardiac fibrogenesisTGFB1 treatmentCardiac fibroblastsGain-of-functionCalcium activityPatientsHF fibroblastsChannel superfamily
2020
Kanglexin, a new anthraquinone compound, attenuates lipid accumulation by activating the AMPK/SREBP-2/PCSK9/LDLR signalling pathway
Li X, Hu X, Pan T, Dong L, Ding L, Wang Z, Song R, Wang X, Wang N, Zhang Y, Wang J, Yang B. Kanglexin, a new anthraquinone compound, attenuates lipid accumulation by activating the AMPK/SREBP-2/PCSK9/LDLR signalling pathway. Biomedicine & Pharmacotherapy 2020, 133: 110802. PMID: 33202286, DOI: 10.1016/j.biopha.2020.110802.Peer-Reviewed Original ResearchMeSH KeywordsAMP-Activated Protein KinasesAnimalsAnthraquinonesBiomarkersDiet, High-FatDisease Models, AnimalFatty LiverHep G2 CellsHepatocytesHumansHyperlipidemiasHypolipidemic AgentsLipidsLiverMaleProprotein Convertase 9RatsRats, Sprague-DawleyReceptors, LDLSignal TransductionSterol Regulatory Element Binding Protein 2ConceptsAdenosine monophosphate-activated protein kinaseSignaling pathwayLipid accumulationOA-treated HepG2 cellsMonophosphate-activated protein kinaseHigh-fat dietHepG2 cellsPhosphorylated adenosine monophosphate-activated protein kinaseHepatic lipid accumulationPotential molecular mechanismsAnthraquinone compoundsCompound CProtein kinaseMolecular mechanismsLipoprotein receptorHepG2 cell modelLipid-lowering effectsOleic acidPathwayCell modelLipidTotal cholesterolHigh-fat diet ratsAccumulationCells
2019
Daming capsule, a hypolipidaemic drug, lowers blood lipids by activating the AMPK signalling pathway
Bian Y, Li X, Li X, Ju J, Liang H, Hu X, Dong L, Wang N, Li J, Zhang Y, Yang B. Daming capsule, a hypolipidaemic drug, lowers blood lipids by activating the AMPK signalling pathway. Biomedicine & Pharmacotherapy 2019, 117: 109176. PMID: 31387185, DOI: 10.1016/j.biopha.2019.109176.Peer-Reviewed Original ResearchConceptsHigh-fat dietDaming capsulePhospho-adenosine monophosphate-activated protein kinaseLevels of TCProtein expression levelsBlood lipidsLDL receptorLDL-CRat modelTotal cholesterolOleic acid-treated HepG2 cellsLDL receptor protein expressionCompound CProtein expressionLow-density lipoprotein cholesterolHepG2 cellsLevels of total cholesterolHigh-fat diet rat liverLipoprotein lipaseExpression levelsHigh-fat diet ratsSerum lipid levelsMonophosphate-activated protein kinaseLiver weight/body weight ratioHigh-fat diet feeding