Edward Manning, MD, PhD
Assistant Professor of Medicine (Pulmonary)Cards
About
Research
Publications
2026
Epidermal growth factor receptor regulates Beclin-1 in hyperoxic acute lung injury
Harris Z, Korde A, Khoury J, Manning E, Stanley G, Shin Y, Mitchell K, von der Schulenburg A, Sun Y, Hu B, Shin H, Joerns J, Clark B, Placek L, Unutmaz D, Moldobaeva A, Sharma L, Sauler M, Rajagopalan G, Zhang X, Wang H, Ghaedi M, Kang M, Koff J. Epidermal growth factor receptor regulates Beclin-1 in hyperoxic acute lung injury. BMJ Open Respiratory Research 2026, 13: e003323. PMID: 41592865, PMCID: PMC12853440, DOI: 10.1136/bmjresp-2025-003323.Peer-Reviewed Original ResearchConceptsEpidermal growth factor receptorAcute lung injuryHyperoxic ALIHyperoxic acute lung injuryGrowth factor receptorIn vivo effectsLung injuryOxidant-mediated acute lung injuryFactor receptorIntensive care unit mortalityExposure to high oxygenAlveolar type II cellsDelivery of supplemental oxygenPulmonary cell deathLife-saving therapyPluripotent stem cellsType II cellsNormoxia controlSupplemental oxygenUnit mortalityAlveolar epitheliumBeclin-1Cell death pathwaysStem cellsTherapeutic potentialProximal Pulmonary Artery Stiffening as a Biomarker of Cardiopulmonary Aging
De Man R, Cai Z, Doddaballapur P, Guerrera N, Regan A, Lin L, Schwarz E, Justet A, Abu Hussein N, Di Palo J, Cavinato C, Raredon M, Heerdt P, Singh I, Yan X, Kang M, Bruns D, Lee P, Tellides G, Humphrey J, Kaminski N, Ramachandra A, Manning E. Proximal Pulmonary Artery Stiffening as a Biomarker of Cardiopulmonary Aging. Aging Cell 2026, 25: e70383. PMID: 41589414, PMCID: PMC12836046, DOI: 10.1111/acel.70383.Peer-Reviewed Original ResearchConceptsProximal pulmonary arteriesPulmonary arterySmooth muscle cellsPerivascular macrophagesMouse modelMuscle cellsRight ventricle functionMedial smooth muscle cellsPulmonary arterial stiffeningRight ventricleVentricle functionAssociated with senescenceECM turnoverLung functionArterial stiffeningArteryAdventitial fibroblastsMolecular targetsAge-relatedGeroscience hypothesisLungAgeIntercellular signalingMiceMacrophagesSingle-cell atlas of human lung aging identifies cell type dyssynchrony and increased transcriptional entropy
De Man R, McDonough J, Adams T, Nikola F, Rangel R, Anderson S, Manning E, Cala Garcia J, Moss B, Waich A, Poli F, Cardenas R, Coarfa C, Song Q, Bar-Joseph Z, Vanaudenaerde B, Wuyts W, Niklason L, Raredon M, Yan X, Rosas I, Kaminski N. Single-cell atlas of human lung aging identifies cell type dyssynchrony and increased transcriptional entropy. Nature Communications 2026, 17: 2095. PMID: 41571679, PMCID: PMC12953888, DOI: 10.1038/s41467-026-68810-9.Peer-Reviewed Original ResearchConceptsGenomic landscapeLung ageSingle-cell atlasAlveolar epithelialIndependent predictorsAnalysis of somatic mutationsSingle-cell dataSingle-cell RNA sequencingEndothelial cellsLoss of differentiationAlveolar epithelial cellsRNA sequencingTranscriptional changesCell-typeEndothelial cell typesSomatic mutationsMutational burdenAT2 cellsLung diseaseCell typesAge-related changesEpithelial cellsRisk factorsSenescence signatureEpithelial
2025
Dysregulated alveolar type 2 epithelial cell proteostasis promotes fibrogenic macrophage migration inhibitory factor–CD74 signaling
Kim S, Nouws J, Ruwisch J, Woodard G, Cooley J, Khoury J, Sun H, Doherty E, Piecychna M, Manning E, Kang M, Bruscia E, Wei H, Zhang Y, Yarovinsky T, Hwa J, Zacharias W, Ingram J, Lee C, Elias J, Kaminski N, Redente E, Herzog E, Prasse A, Bucala R, Sauler M. Dysregulated alveolar type 2 epithelial cell proteostasis promotes fibrogenic macrophage migration inhibitory factor–CD74 signaling. Science Translational Medicine 2025, 17: eadr2277. PMID: 41337540, DOI: 10.1126/scitranslmed.adr2277.Peer-Reviewed Original ResearchMeSH KeywordsAlveolar Epithelial CellsAnimalsAntigens, Differentiation, B-LymphocyteBleomycinDisease Models, AnimalHistocompatibility Antigens Class IIHumansIdiopathic Pulmonary FibrosisIntramolecular OxidoreductasesLungMacrophage Migration-Inhibitory FactorsMacrophagesMaleMiceMice, Inbred C57BLMice, TransgenicProteasome Endopeptidase ComplexProteostasisSignal TransductionUbiquitinConceptsIdiopathic pulmonary fibrosisMacrophage migration inhibitory factorBronchoalveolar lavage fluidPharmacological inhibition of MIFBleomycin-induced lung injury modelMouse modelPharmacological inhibitionC-X3-C motif chemokine receptor 1Human precision-cut lung slicesTransforming growth factor-b1Inhibition of macrophage migration inhibitory factorSignals to macrophagesPrecision-cut lung slicesFibrotic lung diseaseType 2 epithelial cellsAlveolar type 2 epithelial cellsChemokine receptor 1Lung injury modelMigration inhibitory factorMIF-2Study participantsUbiquitin-proteasome systemSpontaneous fibrosisPulmonary fibrosisLavage fluid
2024
Stiffening of the human proximal pulmonary artery with increasing age
Manning E, Mishall P, Ramachandra A, Hassab A, Lamy J, Peters D, Murphy T, Heerdt P, Singh I, Downie S, Choudhary G, Tellides G, Humphrey J. Stiffening of the human proximal pulmonary artery with increasing age. Physiological Reports 2024, 12: e16090. PMID: 38884325, PMCID: PMC11181131, DOI: 10.14814/phy2.16090.Peer-Reviewed Original ResearchConceptsProximal pulmonary arteriesPulmonary arteryAge-related stiffeningRight ventricular ejection fractionVentricular ejection fractionMean wall thicknessEjection fractionNo significant differenceSystemic circulationLuminal caliberDiffusing capacityArterial stiffeningIncreasing ageSignificant differenceDistensionArteryWall thicknessAdverse effectsWall strainVessel diameterCyclic biaxial strainOrgan donorsAgeMicrostructural remodelingRemodeling of Murine Branch Pulmonary Arteries Under Chronic Hypoxia and Short-Term Normoxic Recovery.
Ramachandra A, Jiang B, Jennings I, Manning E, Humphrey J. Remodeling of Murine Branch Pulmonary Arteries Under Chronic Hypoxia and Short-Term Normoxic Recovery. Journal Of Biomechanical Engineering 2024, 146 PMID: 38421341, DOI: 10.1115/1.4064967.Peer-Reviewed Original ResearchWeeks of hypoxiaBranch pulmonary arteriesWeeks of normoxic recoveryPulmonary arteryEndothelial cell responsesSmooth muscleChronic hypoxiaNormoxic recoveryPulmonary arterial remodelingCell responsesProximal pulmonary arteriesLongitudinal changesHypoxia-induced changesC57BL/6J micePulmonary pathologyThin collagen fibersArterial remodelingBiaxial mechanicsCircumferential stressEnergy storageNormal valuesHypoxiaWeeksVascular adaptationFraction of cytoplasmGuillain-Barre in the long-term acute care hospital setting: Ventilation does not prolong stay
Weil K, Baumann V, Brown B, Nadeau R, Hrdlicka H, Gerstenhaber B, Rosenblum D, Manning E. Guillain-Barre in the long-term acute care hospital setting: Ventilation does not prolong stay. Journal Of Mechanical Ventilation 2024, 5: 13-19. DOI: 10.53097/jmv.10094.Peer-Reviewed Original ResearchGuillain-Barre syndrome patientsGuillain-Barre syndromeLong-term acute care hospitalProlonged mechanical ventilationGBS patientsMechanical ventilationLength of stayAcute inflammatory demyelinating polyneuropathyFunctional abilitySevere Guillain-Barre syndromeLong-term acute care hospital settingAcute care hospital settingInflammatory demyelinating polyneuropathyOutcomes of patientsAcute care hospitalsLoss of muscle functionRate of functional improvementTime of admissionMain outcome measuresPoor functional statusDemyelinating polyneuropathyProlonged ventilationRespiratory failureNo significant differenceRetrospective studyDevelopmental changes in lung function of mice are independent of sex as a biological variable
Bärnthaler T, Ramachandra A, Ebanks S, Guerrera N, Sharma L, Dela Cruz C, Humphrey J, Manning E. Developmental changes in lung function of mice are independent of sex as a biological variable. American Journal Of Physiology - Lung Cellular And Molecular Physiology 2024, 326: l627-l637. PMID: 38375577, PMCID: PMC11380952, DOI: 10.1152/ajplung.00120.2023.Peer-Reviewed Original ResearchPulmonary function testsWeeks of ageStatic complianceMale miceInspiratory capacityLung functionBody weight-basedHuman lung pathologiesLung function of miceFemale C57BL/6J miceLung function parametersSex-dependent changesFunction of miceMeasures of lung functionIndependent of sexPreclinical modelsFemale miceC57BL/6J miceLung pathologyFunction testsMiceWeight-basedLungLung disease researchWeeks
2023
Alveolar Vascular Remodeling in Nonspecific Interstitial Pneumonia: Replacement of Normal Lung Capillaries with COL15A1-Positive Endothelial Cells.
Schupp J, Manning E, Chioccioli M, Kamp J, Christian L, Ryu C, Herzog E, Kühnel M, Prasse A, Kaminski N, Jonigk D, Homer R. Alveolar Vascular Remodeling in Nonspecific Interstitial Pneumonia: Replacement of Normal Lung Capillaries with COL15A1-Positive Endothelial Cells. American Journal Of Respiratory And Critical Care Medicine 2023, 208: 819-822. PMID: 37552025, PMCID: PMC10563189, DOI: 10.1164/rccm.202303-0544le.Commentaries, Editorials and LettersThe Burden of COVID-19 Mortality Due to Referrals From Skilled Nursing Facilities in a Small Community Hospital
Reihani A, Vanhouten J, Gill A, Arora K, Manning E. The Burden of COVID-19 Mortality Due to Referrals From Skilled Nursing Facilities in a Small Community Hospital. Cureus 2023, 15: e42369. PMID: 37492036, PMCID: PMC10364562, DOI: 10.7759/cureus.42369.Peer-Reviewed Original ResearchNursing home residentsNursing facilitiesFacility referralSmall community hospitalsCommunity hospitalHome residentsSurge of COVID-19COVID-19-related deathsReferral to hospiceUnequal burdenBurden of COVID-19 mortalityOutcomes of careProportion of patientsSkilled nursing facilityCOVID-19-related admissionsTertiary care facilityHealthcare inequalitiesCare facilitiesNursesCOVID-19 mortalityPotential disparitiesInpatient mortalityClinical characteristicsCOVID-19 deathsLack of published data
Academic Achievements & Community Involvement
Clinical Care
Overview
Edward Manning, MD, PhD, is a specialist in pulmonary and critical care medicine who treats people with lung diseases and breathing challenges. He diagnoses and manages conditions such as persistent shortness of breath and chronic lung disorders, using a range of approaches to improve patients’ well-being.
As an assistant professor of medicine (pulmonary) at Yale School of Medicine, Dr. Manning studies how prolonged low oxygen levels and aging can alter the blood vessels of the lungs and affect heart function. His work aims to develop strategies that support long-term cardiopulmonary health.
Dr. Manning pursued his medical training at Albert Einstein College of Medicine, completed a residency at the Hospital of the University of Pennsylvania, and gained further expertise through a fellowship in pulmonary and critical care medicine at Yale School of Medicine.
Clinical Specialties
Board Certifications
Adult Echocardiography
- Certification Organization
- National Board of Echocardiography
- Original Certification Date
- 2021
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