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Research
Publications
2025
Heterozygous pathogenic variants in the splicing factor SF1 lead to a large spectrum of neurodevelopmental disorders
Bou-Rouphael J, Cospain A, Courtin T, Keren B, Marie C, Lesieur-Sebellin M, Heron D, de Sainte Agathe J, Heide S, Lejeune E, Quelin C, Lecoquierre F, Nizon M, Isidor B, Besnard T, Cogne B, Latypova X, Levy J, Joset P, Steindl K, Palomares-Bralo M, Santos-Simarro F, Thomas M, Abubakar A, Lynch S, Müller A, Haack T, Zenker M, Parker M, Clossick E, Spiller M, Crookes R, Holder-Espinasse M, Bayat A, Møller R, Mieszczanek T, de la Grange P, Buratti J, Marijon P, Ataf S, Gavin R, Parras C, Hassan B, Mignot C, El Khattabi L. Heterozygous pathogenic variants in the splicing factor SF1 lead to a large spectrum of neurodevelopmental disorders. American Journal Of Human Genetics 2025, 112: 2605-2624. PMID: 40987292, PMCID: PMC12808962, DOI: 10.1016/j.ajhg.2025.09.001.Peer-Reviewed Original ResearchConceptsSplicing factor 1Neurodevelopmental disordersAlternative splicingRegulate alternative splicingPre-mRNA processingDysregulation of splicingEarly spliceosome assemblyHeterozygous pathogenic variantsNervous system complexitySpectrum of neurodevelopmental disordersSpliceosome assemblyNon-specific featuresSplice siteSplicing programUnrelated individualsAutistic traitsNeural progenitor cellsPathogenic variantsSplicingAxon guidanceGene expressionHeterozygous variantsFunctional studiesDe novoProgenitor cellsTemporal transcriptional regulation of mitochondrial morphology primes activity-dependent circuit connectivity
Mohylyak I, Andriatsilavo M, Bengochea M, Pascual-Caro C, Asfogo N, Fonseca-Topp S, Danda N, Cassar M, Marie C, Atak Z, De Waegeneer M, Aerts S, Corti O, de Juan-Sanz J, Hassan B. Temporal transcriptional regulation of mitochondrial morphology primes activity-dependent circuit connectivity. Nature Communications 2025, 16: 8173. PMID: 40890095, PMCID: PMC12402207, DOI: 10.1038/s41467-025-62908-2.Peer-Reviewed Original ResearchConceptsMitochondrial morphologyRegulation of mitochondrial morphologyMitochondrial quality control genesTemporal transcriptional regulationDevelopmental transcription factorsNeuronal cell biologyFunctional homologyTranscriptional regulationOrganelle structureGenetic screeningConservation aspectsTranscription factorsControl genesMitochondrial functionDevelopmental programPINK1 expressionCell biologyPINK1Axonal organellesNeurotransmitter releaseHippocampal neuronsSynaptic connectionsDevelopmental downregulationLocal regulationNeuronal connectivitySequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster
Andriatsilavo M, Barata C, Reifenstein E, Dumoulin A, Tao Griffin T, Dutta S, Stoeckli E, von Kleist M, Hiesinger P, Hassan B. Sequential and independent probabilistic events regulate differential axon targeting during development in Drosophila melanogaster. Nature Neuroscience 2025, 28: 998-1011. PMID: 40335773, DOI: 10.1038/s41593-025-01937-y.Peer-Reviewed Original ResearchToward a probabilistic definition of neural cell types
Andriatsilavo M, Hassan B. Toward a probabilistic definition of neural cell types. Current Opinion In Neurobiology 2025, 92: 103035. PMID: 40334296, DOI: 10.1016/j.conb.2025.103035.Peer-Reviewed Original ResearchAuthor Correction: Pharmacogenomic screening identifies and repurposes leucovorin and dyclonine as pro-oligodendrogenic compounds in brain repair
Huré J, Foucault L, Ghayad L, Marie C, Vachoud N, Baudouin L, Azmani R, Ivljanin N, Arevalo-Nuevo A, Pigache M, Bouslama-Oueghlani L, Chemelle J, Dronne M, Terreux R, Hassan B, Gueyffier F, Raineteau O, Parras C. Author Correction: Pharmacogenomic screening identifies and repurposes leucovorin and dyclonine as pro-oligodendrogenic compounds in brain repair. Nature Communications 2025, 16: 538. PMID: 39788999, PMCID: PMC11717921, DOI: 10.1038/s41467-025-55864-4.Peer-Reviewed Original Research
2024
Pharmacogenomic screening identifies and repurposes leucovorin and dyclonine as pro-oligodendrogenic compounds in brain repair
Huré J, Foucault L, Ghayad L, Marie C, Vachoud N, Baudouin L, Azmani R, Ivljanin N, Arevalo-Nuevo A, Pigache M, Bouslama-Oueghlani L, Chemelle J, Dronne M, Terreux R, Hassan B, Gueyffier F, Raineteau O, Parras C. Pharmacogenomic screening identifies and repurposes leucovorin and dyclonine as pro-oligodendrogenic compounds in brain repair. Nature Communications 2024, 15: 9837. PMID: 39537633, PMCID: PMC11561360, DOI: 10.1038/s41467-024-54003-9.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBrainBrain InjuriesCell DifferentiationCell ProliferationDisease Models, AnimalDrug RepositioningFemaleHumansLeucovorinMaleMiceMice, Inbred C57BLMicrogliaMultiple SclerosisMyelin SheathNeural Stem CellsOligodendrocyte Precursor CellsOligodendrogliaPharmacogenomic TestingRemyelinationConceptsOligodendrocyte progenitor cellsIn Vivo Mouse ModelMultiple sclerosisOligodendrocyte progenitor cell differentiationMyelin debris clearanceProgenitor cell proliferationNeural progenitor cell proliferationPharmacogenomic screeningPharmacogenomic approachLeucovorinProgenitor cellsClinical trialsMyelin disordersMouse modelPro-inflammatoryEffective treatmentBrain repairTherapeutic avenuesCerebellar explantsCell proliferationLesion repairIn vitroDebris clearanceMyelin formationTranscriptional programsTiming neurogenesis: a clock or an algorithm?
Pigeon J, Hassan B. Timing neurogenesis: a clock or an algorithm? Current Opinion In Genetics & Development 2024, 85: 102156. PMID: 38354530, DOI: 10.1016/j.gde.2024.102156.Peer-Reviewed Original Research
2023
A New Behavioral Paradigm for Visual Classical Conditioning in Drosophila
Bengochea M, Preat T, Hassan B. A New Behavioral Paradigm for Visual Classical Conditioning in Drosophila. Bio-protocol 2023, 13: e4875. PMID: 37969763, PMCID: PMC10632160, DOI: 10.21769/bioprotoc.4875.Peer-Reviewed Original ResearchCognitive abilitiesVisual learningBehavioral assaysVisual associative learningFruit flyAppetitive rewardBehavioral paradigmsAssociative learningClassical conditioningVisual settingTest sessionsAdult fruit fliesNumerical informationLearning abilityRewardWalking fruit fliesLearning protocolIndividual levelCentral nervous systemLearningFliesOrientation behaviorNumerical discrimination in Drosophila melanogaster
Bengochea M, Sitt J, Izard V, Preat T, Cohen L, Hassan B. Numerical discrimination in Drosophila melanogaster. Cell Reports 2023, 42: 112772. PMID: 37453418, PMCID: PMC10442639, DOI: 10.1016/j.celrep.2023.112772.Peer-Reviewed Original ResearchConceptsNumerical cognitionNumerical quantitiesConditioning paradigmNumerical discriminationSensitive to numberNeural mechanismsCognitive abilitiesNeuronal substratesSpontaneous preferenceNeural manipulationNeural circuitsCognitionDrosophila melanogasterConsecutive daysDrosophila fliesIndividual fliesNumerosityColumnar neuronsLack of experimental modelsPreferencesFliesParadigmNeuronsDiscriminationInvertebratesThe temporal balance between self-renewal and differentiation of human neural stem cells requires the amyloid precursor protein
Shabani K, Pigeon J, Zariouh M, Liu T, Saffarian A, Komatsu J, Liu E, Danda N, Becmeur-Lefebvre M, Limame R, Bohl D, Parras C, Hassan B. The temporal balance between self-renewal and differentiation of human neural stem cells requires the amyloid precursor protein. Science Advances 2023, 9: eadd5002. PMID: 37327344, PMCID: PMC10275593, DOI: 10.1126/sciadv.add5002.Peer-Reviewed Original ResearchConceptsAmyloid precursor proteinPrecursor proteinNeural progenitor cellsTemporal patterns of neurogenesisProgenitor stateActivator protein-1 transcription factorSelf-RenewalMouse neural progenitor cellsTranscription factorsCell-autonomousHuman neural progenitor cellsHuman neural stem cellsCortical neural progenitorsDifferentiation of human neural stem cellsPattern of neurogenesisWnt signalingNeural stem cellsProgenitor cellsAmyloidNeural progenitorsStem cellsCerebral cortexHuman cerebral cortexProteinNeurogenesis