Shiv Gandhi, MD, PhD
About
Research
Publications
Featured Publications
De novo emergence of a remdesivir resistance mutation during treatment of persistent SARS-CoV-2 infection in an immunocompromised patient: a case report
Gandhi S, Klein J, Robertson AJ, Peña-Hernández MA, Lin MJ, Roychoudhury P, Lu P, Fournier J, Ferguson D, Mohamed Bakhash SAK, Catherine Muenker M, Srivathsan A, Wunder EA, Kerantzas N, Wang W, Lindenbach B, Pyle A, Wilen CB, Ogbuagu O, Greninger AL, Iwasaki A, Schulz WL, Ko AI. De novo emergence of a remdesivir resistance mutation during treatment of persistent SARS-CoV-2 infection in an immunocompromised patient: a case report. Nature Communications 2022, 13: 1547. PMID: 35301314, PMCID: PMC8930970, DOI: 10.1038/s41467-022-29104-y.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 infectionVirologic responsePersistent SARS-CoV-2 infectionResistance mutationsPre-treatment specimensB-cell deficiencyRemdesivir resistanceRemdesivir therapyViral sheddingCase reportAntiviral agentsPatientsCombinatorial therapyInfectionTherapyWhole-genome sequencingTreatmentImportance of monitoringDe novo emergenceFold increaseRNA-dependent RNA polymeraseNovo emergencePotential benefitsMutationsIndolent
2023
Lymphocytic Choriomeningitis Virus in Person Living with HIV, Connecticut, USA, 2021 - Volume 29, Number 9—September 2023 - Emerging Infectious Diseases journal - CDC
Dyal J, Gandhi S, Cossaboom C, Leach A, Patel K, Golden M, Canterino J, Landry M, Cannon D, Choi M, Krapiunaya I, Klena J, Shoemaker T. Lymphocytic Choriomeningitis Virus in Person Living with HIV, Connecticut, USA, 2021 - Volume 29, Number 9—September 2023 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2023, 29: 1886-1889. PMID: 37610188, PMCID: PMC10461659, DOI: 10.3201/eid2909.230087.Peer-Reviewed Original Research
2022
Inhaled Bacteriophage Therapy for Multi-Drug Resistant Achromobacter.
Winzig F, Gandhi S, Lee A, Würstle S, Stanley G, Capuano I, Neuringer I, Koff J, Turner P, Chan B. Inhaled Bacteriophage Therapy for Multi-Drug Resistant Achromobacter. The Yale Journal Of Biology And Medicine 2022, 95: 413-427. PMID: 36568830, PMCID: PMC9765334.Peer-Reviewed Original ResearchConceptsCF patientsCystic fibrosisChronic pulmonary infectionGlobal public health threatBacterial lung infectionsChallenging clinical problemPublic health threatChronic bacterial lung infectionsPulmonary infectionRespiratory statusLung infectionClinical problemBacteriophage therapyInfectionAntimicrobial-resistant bacteriaTherapyHealth threatPhage therapyPatientsAMR infectionsResistant bacteriaLytic bacteriophagesPossible benefitsChemical antibioticsCurrent studyBorrelia miyamotoi Meningoencephalitis in an Immunocompetent Patient
Gandhi S, Narasimhan S, Workineh A, Mamula M, Yoon J, Krause PJ, Farhadian SF. Borrelia miyamotoi Meningoencephalitis in an Immunocompetent Patient. Open Forum Infectious Diseases 2022, 9: ofac295. PMID: 35873293, PMCID: PMC9301576, DOI: 10.1093/ofid/ofac295.Peer-Reviewed Original Research
2020
Designed Parasite-Selective Rhomboid Inhibitors Block Invasion and Clear Blood-Stage Malaria
Gandhi S, Baker R, Cho S, Stanchev S, Strisovsky K, Urban S. Designed Parasite-Selective Rhomboid Inhibitors Block Invasion and Clear Blood-Stage Malaria. Cell Chemical Biology 2020, 27: 1410-1424.e6. PMID: 32888502, PMCID: PMC7680425, DOI: 10.1016/j.chembiol.2020.08.011.Peer-Reviewed Original ResearchConceptsRhomboid proteolysisRhomboid intramembrane proteasesAtypical substrate specificityHost cell invasionHigh-resolution structuresRhomboid proteasesIntramembrane proteasesRhomboid inhibitorsSubstrate specificityBlocks invasionRapid invasionDisease contextsSteric clashesProteolysisUltrastructural analysisProteaseInvasionPathophysiological processesPeptide boronatesBlood-stage malariaSteric exclusionParasitesMalaria culturesSequenceTargeting
2015
A Trichomonas vaginalis Rhomboid Protease and Its Substrate Modulate Parasite Attachment and Cytolysis of Host Cells
Riestra A, Gandhi S, Sweredoski M, Moradian A, Hess S, Urban S, Johnson P. A Trichomonas vaginalis Rhomboid Protease and Its Substrate Modulate Parasite Attachment and Cytolysis of Host Cells. PLOS Pathogens 2015, 11: e1005294. PMID: 26684303, PMCID: PMC4684317, DOI: 10.1371/journal.ppat.1005294.Peer-Reviewed Original ResearchConceptsT. vaginalis pathogenesisRhomboid proteasesHost cellsCell surface membrane proteinsIntramembrane serine proteasesDifferent subcellular localizationsSurface membrane proteinsAmino acid residuesSmall amino acidsEukaryotic parasitesTransmembrane domainBioinformatics approachMembrane proteinsSubcellular localizationSurface proteomeSubstrate specificityMolecular mechanismsGolgi apparatusAcid residuesProtease substratesCleavage assaysCleavage siteAmino acidsCell surfaceParasite attachmentAn Aminopeptidase in the Drosophila Testicular Niche Acts in Germline Stem Cell Maintenance and Spermatogonial Dedifferentiation
Lim C, Gandhi S, Biniossek M, Feng L, Schilling O, Urban S, Chen X. An Aminopeptidase in the Drosophila Testicular Niche Acts in Germline Stem Cell Maintenance and Spermatogonial Dedifferentiation. Cell Reports 2015, 13: 315-325. PMID: 26440886, PMCID: PMC4607668, DOI: 10.1016/j.celrep.2015.09.001.Peer-Reviewed Original ResearchConceptsGermline stem cellsDE-cadherinCell maintenanceTesticular nicheGermline stem cell maintenanceStem cell maintenanceCell dedifferentiationStem cellsAdult stem cellsSpecific aminopeptidase activityMutant phenotypeSpermatogonial DedifferentiationStem cell lossNiche factorsVivo functionExtrinsic cuesNicheAminopeptidase activityDedifferentiationImportant targetCellsMaintenanceOverexpressionMutationsPhenotype
2013
Evolution of an Attenuated HIV-1 Isolate in an Elite Suppressor
Salgado M, Gandhi S, Buckheit R, Berkenblit G, Blankson J. Evolution of an Attenuated HIV-1 Isolate in an Elite Suppressor. AIDS Research And Human Retroviruses 2013, 30: 284-288. PMID: 24117037, PMCID: PMC3938915, DOI: 10.1089/aid.2013.0229.Peer-Reviewed Original Research
2007
Role of APOBEC3G/F-Mediated Hypermutation in the Control of Human Immunodeficiency Virus Type 1 in Elite Suppressors▿
Gandhi S, Siliciano J, Bailey J, Siliciano R, Blankson J. Role of APOBEC3G/F-Mediated Hypermutation in the Control of Human Immunodeficiency Virus Type 1 in Elite Suppressors▿. Journal Of Virology 2007, 82: 3125-3130. PMID: 18077705, PMCID: PMC2259015, DOI: 10.1128/jvi.01533-07.Peer-Reviewed Original ResearchConceptsElite suppressorsAntiretroviral therapyHuman immunodeficiency virus type 1 (HIV-1) replicationHIV-1-infected individualsHuman immunodeficiency virus type 1Immunodeficiency virus type 1Control of viremiaActive antiretroviral therapyType 1 replicationVirus type 1Host defense mechanismsViral loadCopies/Clinical significanceAPOBEC3 activityType 1APOBEC3 familyViremiaPatientsTherapyProviral genomeCytidine deaminasesF proteinDefense mechanismsNovel assay
2006
Isolation and Characterization of Replication-Competent Human Immunodeficiency Virus Type 1 from a Subset of Elite Suppressors▿
Blankson J, Bailey J, Thayil S, Yang H, Lassen K, Lai J, Gandhi S, Siliciano J, Williams T, Siliciano R. Isolation and Characterization of Replication-Competent Human Immunodeficiency Virus Type 1 from a Subset of Elite Suppressors▿. Journal Of Virology 2006, 81: 2508-2518. PMID: 17151109, PMCID: PMC1865922, DOI: 10.1128/jvi.02165-06.Peer-Reviewed Original ResearchConceptsReplication-competent HIV-1Human immunodeficiency virus type 1Immunodeficiency virus type 1Elite suppressorsVirus type 1HIV-1Replication-Competent Human Immunodeficiency Virus Type 1Untreated human immunodeficiency virus type 1Type 1Active antiretroviral therapyUndetectable viral loadReplication-competent virusAntiretroviral therapyImmunologic controlViral loadCoculture assaysPatientsDefective virusCurrent assaysFull-length sequencingVirusIsolatesAssaysPhenotypic analysisViremia