Shan Parikh, MD, PhD
About
Research
Publications
Featured Publications
Impaired Dynamic Sarcoplasmic Reticulum Ca Buffering in Autosomal Dominant CPVT2
Wleklinski M, Kryshtal D, Kim K, Parikh S, Blackwell D, Marty I, Iyer V, Knollmann B. Impaired Dynamic Sarcoplasmic Reticulum Ca Buffering in Autosomal Dominant CPVT2. Circulation Research 2022, 131: 673-686. PMID: 36102198, PMCID: PMC9529867, DOI: 10.1161/circresaha.121.320661.Peer-Reviewed Original ResearchPatient-independent human induced pluripotent stem cell model: A new tool for rapid determination of genetic variant pathogenicity in long QT syndrome
Chavali NV, Kryshtal DO, Parikh SS, Wang L, Glazer AM, Blackwell DJ, Kroncke BM, Shoemaker MB, Knollmann BC. Patient-independent human induced pluripotent stem cell model: A new tool for rapid determination of genetic variant pathogenicity in long QT syndrome. Heart Rhythm 2019, 16: 1686-1695. PMID: 31004778, PMCID: PMC6935564, DOI: 10.1016/j.hrthm.2019.04.031.Peer-Reviewed Original ResearchConceptsLong QT syndromeExtracellular field potentialsQT syndromeHiPSC-CMGenetic testingPluripotent stem cell modelsHiPSC modelsSudden cardiac deathStem cell modelUnrelated healthy volunteersVentricular action potentialPatch-clamp studiesNovel missense variantVoltage-dependent inactivationCardiac deathClinical benefitCommercial genetic testingGenetic variant pathogenicityHealthy volunteersCell modelElectrophysiological propertiesAction potentialsBeating rateUnknown significancePatch clampThyroid and Glucocorticoid Hormones Promote Functional T-Tubule Development in Human-Induced Pluripotent Stem Cell–Derived Cardiomyocytes
Parikh SS, Blackwell DJ, Gomez-Hurtado N, Frisk M, Wang L, Kim K, Dahl CP, Fiane A, Tønnessen T, Kryshtal DO, Louch WE, Knollmann BC. Thyroid and Glucocorticoid Hormones Promote Functional T-Tubule Development in Human-Induced Pluripotent Stem Cell–Derived Cardiomyocytes. Circulation Research 2017, 121: 1323-1330. PMID: 28974554, PMCID: PMC5722667, DOI: 10.1161/circresaha.117.311920.Peer-Reviewed Original ResearchConceptsHuman-induced pluripotent stem cell-derived cardiomyocytesExcitation-contraction couplingPluripotent stem cell-derived cardiomyocytesCa releaseGlucocorticoid hormonesStem cell-derived cardiomyocytesCell-derived cardiomyocytesCa-induced Ca releaseT-tubule developmentAdult human ventricular cardiomyocytesExtensive T-tubule networkHuman ventricular cardiomyocytesL-type Ca channelsL-type CaT-tubulesIntracellular Ca releaseCell-based therapiesConfocal line scanHeart diseaseT-tubule networkFunctional maturationVentricular cardiomyocytesPermissive roleDay 16Ca channels
2023
Regulation of Postnatal Cardiomyocyte Maturation by an RNA Splicing Regulator RBFox1
Huang J, Lee J, Rau C, Pezhouman A, Yokota T, Miwa H, Feldman M, Kong T, Yang Z, Tay W, Pushkarsky I, Kim K, Parikh S, Udani S, Soh B, Gao C, Stiles L, Shirihai O, Knollmann B, Ardehali R, Di Carlo D, Wang Y. Regulation of Postnatal Cardiomyocyte Maturation by an RNA Splicing Regulator RBFox1. Circulation 2023, 148: 1263-1266. PMID: 37844148, PMCID: PMC10593507, DOI: 10.1161/circulationaha.122.061602.Peer-Reviewed Original Research
2022
Transcriptional Dysregulation Underlies Both Monogenic Arrhythmia Syndrome and Common Modifiers of Cardiac Repolarization
Bersell K, Yang T, Mosley J, Glazer A, Hale A, Kryshtal D, Kim K, Steimle J, Brown J, Salem J, Campbell C, Hong C, Wells Q, Johnson A, Short L, Blair M, Behr E, Petropoulou E, Jamshidi Y, Benson M, Keyes M, Ngo D, Vasan R, Yang Q, Gerszten R, Shaffer C, Parikh S, Sheng Q, Kannankeril P, Moskowitz I, York J, Wang T, Knollmann B, Roden D. Transcriptional Dysregulation Underlies Both Monogenic Arrhythmia Syndrome and Common Modifiers of Cardiac Repolarization. Circulation 2022, 147: 824-840. PMID: 36524479, PMCID: PMC9992308, DOI: 10.1161/circulationaha.122.062193.Peer-Reviewed Original ResearchConceptsPlatelet-derived growth factorInduced pluripotent stem cellsBrugada syndromeArrhythmia syndromesSerum platelet-derived growth factorSodium currentGeneral transcriptional mechanismFramingham Heart Study cohortPI3KPDGF receptor expressionLate sodium currentCardiac sodium currentCardiac transcription factorsSmall molecule perturbationsCurrent-clamp experimentsCardiac sodium channel geneSodium channel geneFramingham Heart StudyMurine model systemPluripotent stem cellsMonogenic arrhythmia syndromesReceptor blockadeElectrophysiologic abnormalitiesQTc intervalStudy cohortAutosomal dominant CPVT2 is caused by impaired dynamic calcium buffering in the sarcoplasmic reticulum
Wleklinski M, Parikh S, Kryshtal D, Knollmann B. Autosomal dominant CPVT2 is caused by impaired dynamic calcium buffering in the sarcoplasmic reticulum. Biophysical Journal 2022, 121: 12a-13a. DOI: 10.1016/j.bpj.2021.11.2644.Peer-Reviewed Original Research
2021
Autosomal-Dominant CASQ2-K180R Causes CPVT by Altering Intra-SR Calcium Buffering without Reducing Casq2 Protein Levels
Wleklinski M, Parikh S, Blackwell D, Knollmann B. Autosomal-Dominant CASQ2-K180R Causes CPVT by Altering Intra-SR Calcium Buffering without Reducing Casq2 Protein Levels. Biophysical Journal 2021, 120: 238a-239a. DOI: 10.1016/j.bpj.2020.11.1572.Peer-Reviewed Original Research
2020
Autosomal-dominant CASQ2-K180R Causes CPVT by a Different Mechanism than Autosomal-recessive Casq2 Mutations
Wleklinski M, Parikh S, Knollmann B. Autosomal-dominant CASQ2-K180R Causes CPVT by a Different Mechanism than Autosomal-recessive Casq2 Mutations. Biophysical Journal 2020, 118: 101a. DOI: 10.1016/j.bpj.2019.11.709.Peer-Reviewed Original Research
2019
An Autosomal Dominant Mutation in Calsequestrin 2 Causes CPVT Without Changing Protein Levels
Wleklinski M, Parikh S, Knollmann B. An Autosomal Dominant Mutation in Calsequestrin 2 Causes CPVT Without Changing Protein Levels. Biophysical Journal 2019, 116: 95a-96a. DOI: 10.1016/j.bpj.2018.11.556.Peer-Reviewed Original ResearchEstablishing Pathogenicity of Novel LQTS8 Variant via Genomic Editing of Human iPSC
Kryshtal D, Chavali N, Parikh S, Wang L, Glazer A, Shoemaker M, Knollmann B. Establishing Pathogenicity of Novel LQTS8 Variant via Genomic Editing of Human iPSC. Biophysical Journal 2019, 116: 99a. DOI: 10.1016/j.bpj.2018.11.573.Peer-Reviewed Original Research
Links & Media
News
- August 03, 2023
Emergency Medicine Residency Program Consultant of the Month Goes to Parikh
- March 13, 2023
Fellow Focus in Four: Shan Parikh, MD, PhD, Cardiovascular Medicine
- August 10, 2022
Welcome New Staff, Faculty, Fellows, Postgrads (August 2022)
- November 30, 2021
Yale Cardiovascular Medicine Announces 2022 Match Class