Matthew Puccetti, MD, PhD
Assistant ProfessorCards
About
Research
Publications
2024
CHANTER syndrome: an under-recognized cause of reversible hydrocephalus after opioid overdose
Sotiropoulos M, Harrison D, Zusman B, Puccetti M, Molyneaux B. CHANTER syndrome: an under-recognized cause of reversible hydrocephalus after opioid overdose. Neurological Sciences 2024, 46: 2353-2356. PMID: 39730951, DOI: 10.1007/s10072-024-07979-7.Peer-Reviewed Original ResearchDNA fork remodeling proteins, Zranb3 and Smarcal1, are uniquely essential for aging hematopoiesis
Kushinsky S, Puccetti M, Adams C, Shkundina I, James N, Mahon B, Michener P, Eischen C. DNA fork remodeling proteins, Zranb3 and Smarcal1, are uniquely essential for aging hematopoiesis. Aging Cell 2024, 23: e14281. PMID: 39044358, PMCID: PMC11561652, DOI: 10.1111/acel.14281.Peer-Reviewed Original ResearchConceptsReplication stressDNA damageChromatin remodeling enzymesLifelong hematopoiesisReplication fork dynamicsLong-term HSC functionCell-intrinsic functionRemodeling enzymesRemodeling proteinsBiochemical functionsZRANB3Bone marrow transplantationHSPC defectsProgenitor cell functionSMARCAL1DNABiological consequencesHematopoiesis defectsLymphoid-biasedMarrow transplantationHSC functionSimultaneous lossHematopoietic stemProteinCell function
2022
Targeting chemotherapy to de-condensed H3K27me3-marked chromatin of AML cells enhances leukemia suppression
Porazzi P, Petruk S, Pagliaroli L, De Dominici M, Deming D, Puccetti M, Kushinsky S, Kumar G, Minieri V, Barbieri E, Deliard S, Grande A, Trizzino M, Gardini A, Canaani E, Palmisiano N, Porcu P, Ertel A, Fortina P, Eischen C, Mazo A, Calabretta B. Targeting chemotherapy to de-condensed H3K27me3-marked chromatin of AML cells enhances leukemia suppression. Cancer Research 2022, 82: canres.1297.2021. PMID: 34903608, PMCID: PMC8889548, DOI: 10.1158/0008-5472.can-21-1297.Peer-Reviewed Original ResearchConceptsAcute myelogenous leukemiaLeukemia suppressionChromatin decondensationAML cellsRepressive histone mark H3K27me3Reduced doseInduce transcriptional reprogrammingHistone mark H3K27me3Reduced doses of chemotherapyCytotoxic agentsCell death-promotingPharmacological inhibitionDNA-damaging cytotoxic agentsGrowth-inhibitory genesPharmacological inhibition of EZH2Cell linesS-phase entryChemotherapy-induced DNA damageDoses of chemotherapyAcute myelogenous leukemia cellsTreatment-related side effectsHistone methyltransferase EZH2AML cell linesXenograft mouse modelChromatin accessibility
2020
Loss of the DNA Fork Remodeling Protein Smarcal1 Impairs the Replication Stress Response in Proliferating Hematopoietic Cells
Kushinsky S, Puccetti M, Adams C, Eischen C. Loss of the DNA Fork Remodeling Protein Smarcal1 Impairs the Replication Stress Response in Proliferating Hematopoietic Cells. Blood 2020, 136: 34. DOI: 10.1182/blood-2020-140354.Peer-Reviewed Original ResearchBone marrow transplantationSchimke immuno-osseous dysplasiaMarrow transplantationHematopoietic cellsHematopoietic cell populationsBone marrowPatients susceptible to opportunistic infectionsMature hematopoietic cell populationsWild-type bone marrowSusceptibility to opportunistic infectionsCell populationsImmunodeficiency of patientsLow-dose radiationProliferating hematopoietic cellsBone marrow environmentImmuno-osseous dysplasiaLevels of replication stressKnockout mouse modelDNA replication stressIn vivo functionHematopoietic cell survivalReplication stressReplication stress responseSIOD patientsOpportunistic infections
2019
IL-33 Is a Cell-Intrinsic Regulator of Fitness during Early B Cell Development
Stier M, Mitra R, Nyhoff L, Goleniewska K, Zhang J, Puccetti M, Casanova H, Seegmiller A, Newcomb D, Kendall P, Eischen C, Peebles R. IL-33 Is a Cell-Intrinsic Regulator of Fitness during Early B Cell Development. The Journal Of Immunology 2019, 203: 1457-1467. PMID: 31391233, PMCID: PMC6736727, DOI: 10.4049/jimmunol.1900408.Peer-Reviewed Original ResearchConceptsEarly B cell developmentC-terminal domainB cell developmentRNA sequencing analysisCell-intrinsic regulatorPrecursor B cellsIL-33 expressionIL-33 deficiencyIL-33B cellsAnalysis of wild-typeN-terminal domainExtracellular releaseFamily member proteinsReceptor ST2Cell cycle genesCell-intrinsicB-cell chronic lymphocytic leukemia samplesCD4+ Th2 cellsBone marrow chimeric micePro-B cell stageIL-33 receptor ST2Release of IL-33Chronic lymphocytic leukemia samplesChromatin bindingMicroRNA-21 is Required for Hematopoietic Cell Viability After Radiation Exposure
Puccetti M, Adams C, Dan T, Palagani A, Simone B, DeAngelis T, Eischen C, Simone N. MicroRNA-21 is Required for Hematopoietic Cell Viability After Radiation Exposure. International Journal Of Radiation Oncology • Biology • Physics 2019, 104: 1165-1174. PMID: 31039423, PMCID: PMC6986889, DOI: 10.1016/j.ijrobp.2019.04.020.Peer-Reviewed Original ResearchConceptsTotal body irradiationMiR-21Radiation-induced toxicityWild-type miceMiR-21-deficient miceEvidence of bone marrow failureMiR-21 levelsNormal tissue response to irradiationRisk of radiation-induced toxicityMiR-21 expressionTransplantation of wild-type bone marrowConcurrent use of radiationTissue response to irradiationDose of total body irradiationWild-type bone marrowConcurrent useHypofractionated radiation regimensBone marrow recoveryBone marrow transplantationBone marrow failureComplete blood cell countHematopoietic cell viabilityNon-coding RNAsBlood cell countMicroRNA-21Smarcal1 and Zranb3 protect replication forks from Myc-induced DNA replication stress
Puccetti M, Adams C, Kushinsky S, Eischen C. Smarcal1 and Zranb3 protect replication forks from Myc-induced DNA replication stress. Cancer Research 2019, 79: canres.2705.2018. PMID: 30610086, PMCID: PMC6445766, DOI: 10.1158/0008-5472.can-18-2705.Peer-Reviewed Original ResearchConceptsDNA replication stressReplication forksReplication stressMyc-induced lymphoma developmentDNA replication stress responseReplication stress responseDNA replication forksReplication fork stallingStabilize replication forksPrimary cellsEndogenous replication stressNonredundant functionsOncogenic stressFork stallingGenomic instabilityMYC overexpressionZRANB3Stress responseSMARCAL1Nonredundant mannerAccelerated lymphomagenesisMYC dysregulationDNA damageMYCLymphoma development
2016
Defective replication stress response inhibits lymphomagenesis and impairs lymphocyte reconstitution
Puccetti M, Fischer M, Arrate M, Boyd K, Duszynski R, Bétous R, Cortez D, Eischen C. Defective replication stress response inhibits lymphomagenesis and impairs lymphocyte reconstitution. Oncogene 2016, 36: 2553-2564. PMID: 27797382, PMCID: PMC5562353, DOI: 10.1038/onc.2016.408.Peer-Reviewed Original ResearchConceptsHematopoietic stem/progenitor cellsReplication stress responseStress responseReplication stressDNA replication stress responseLymphoma mouse modelDevelopment of malignancyHematopoietic cell survivalDNA replication forksDNA replication stressIn vivo functionReplication fork collapseLymphocyte reconstitutionStem/progenitor cellsTumor developmentMouse modelReplication forksGenomic instabilityDNA breaksFork collapseCell survivalMiceDNASMARCAL1DNA damage
2011
Dynamic regulation of aquaglyceroporin expression in erythrocyte cultures from cold‐ and warm‐acclimated cope's gray treefrog, Hyla chrysoscelis
Mutyam V, Puccetti M, Frisbie J, Goldstein D, Krane C. Dynamic regulation of aquaglyceroporin expression in erythrocyte cultures from cold‐ and warm‐acclimated cope's gray treefrog, Hyla chrysoscelis. Journal Of Experimental Zoology Part A Ecological And Integrative Physiology 2011, 315A: 424-437. PMID: 21656914, DOI: 10.1002/jez.689.Peer-Reviewed Original ResearchConceptsWarm-acclimated frogsGray treefrogsHyla chrysoscelisCell culture systemCope's gray treefrogFreeze-tolerant anuransTemperature-dependent regulationPlasma membrane localizationCold-acclimated frogsN-linked glycosylationH. chrysoscelisSubcellular traffickingGene regulationPost-translationallyMIP familyMembrane localizationProtein abundanceFreezing toleranceIncreased abundanceDynamic regulationProperties of cellsCulture systemCold acclimationCellular localizationTransmembrane passageEndo-Portermediated delivery of phosphorodiamidate morpholino oligos (PMOs) in erythrocyte suspension cultures from Cope's gray treefrog Hyla chrysoscelis
Mutyam V, Puccetti M, Frisbie J, Goldstein D, Krane C. Endo-Portermediated delivery of phosphorodiamidate morpholino oligos (PMOs) in erythrocyte suspension cultures from Cope's gray treefrog Hyla chrysoscelis. BioTechniques 2011, 50: 329-332. PMID: 21548895, DOI: 10.2144/000113671.Peer-Reviewed Original ResearchConceptsHyla chrysoscelisPhosphorodiamidate morpholino oligoCope's gray treefrogFreeze-tolerant anuransEfficient knockdownEndo-PorterH. chrysoscelisGray treefrogsKnockout toolsMorpholino oligosKnockdown approachCold acclimationCryoprotectant glycerolPharmacological inhibitorsSuspension culturesChrysoscelisDetectable expressionProtein expressionWestern blottingAquaglyceroporinExpressionPre-incubationContinuous shakingPhysiological temperatureTreefrogs
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