Jan Parolek
Associate Research Scientist in Cell BiologyAbout
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2026
Cholesterol depletion activates trafficking-coupled sphingolipid synthesis
Kim Y, Parolek J, Burd C. Cholesterol depletion activates trafficking-coupled sphingolipid synthesis. Journal Of Cell Biology 2026, 225: e202502083. PMID: 41556882, DOI: 10.1083/jcb.202502083.Peer-Reviewed Original ResearchConceptsEndoplasmic reticulum-to-Golgi traffickingGolgi apparatusLipid composition of organelle membranesEndoplasmic reticulum exit sitesVLC ceramidesTrafficking of ceramideEndoplasmic reticulum networkIntegral membrane proteinsAcute depletionLipid compositionSynthetic ceramide analogCTAGE5Depletion of cell cholesterolTrafficking assaySphingolipid synthesisCeramide analogsReticulum networkOrganelle membranesEndoplasmic reticulumMembrane proteinsSphingomyelin synthesisPlasma membraneCell cholesterolLipid sensingCholesterol depletion
2024
Bridge-like lipid transfer protein family member 2 suppresses ciliogenesis
Parolek J, Burd C. Bridge-like lipid transfer protein family member 2 suppresses ciliogenesis. Molecular Biology Of The Cell 2024, 35: br11. PMID: 38536441, PMCID: PMC11151097, DOI: 10.1091/mbc.e24-02-0065.Peer-Reviewed Original ResearchConceptsLipid transfer proteinsFamily member 2RPE-1 cellsSuppressed ciliogenesisTubular endosomal networkMembrane contact sitesNegative regulator of ciliogenesisRegulator of ciliogenesisDrosophila melanogasterEvolutionary conserved proteinMember 2Primary cilium biogenesisRPE-1Endosomal networkGenetic interactionsTubular endosomesCilium biogenesisProtein familyStructure predictionContact sitesEndoplasmic reticulumDomain-containingPreweaning lethalityNegative regulatorCiliogenesis
2022
Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathway
Sokoya T, Parolek J, Foged M, Danylchuk D, Bozan M, Sarkar B, Hilderink A, Philippi M, Botto L, Terhal P, Mäkitie O, Piehler J, Kim Y, Burd C, Klymchenko A, Maeda K, Holthuis J. Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathway. ELife 2022, 11: e79278. PMID: 36102623, PMCID: PMC9531943, DOI: 10.7554/elife.79278.Peer-Reviewed Original ResearchConceptsSecretory pathwayER export signalPatient-derived fibroblastsFull enzymatic activityPhysical membrane propertiesExport signalSterol gradientLipid landscapeMammalian cellsCholesterol organizationLipid orderEnzymatic activityLipid distributionOsteogenic cellsPathwayBone phenotypeSMS2Glycerophospholipid profilePathogenic variantsCellsVariantsSphingomyelinSkeletal dysplasiaERSphingolipids
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