Irradiator Shared Resource

Transcript

• 00:00My name is Kamlakram Baripuri.
• 00:02I manage the Irvator shared
• 00:04resource, resource center here. We're
• 00:06part of the cancer center
• 00:07support,
• 00:08grant share resources.
• 00:12ISR is supervised by doctor
• 00:14Stern,
• 00:15and reviewed annually by the
• 00:16YCC shared, oversight committee.
• 00:21The shared resource is managed
• 00:22by doc director doctor Jay
• 00:24Chen.
• 00:26He is the
• 00:27director of ISR. He's a
• 00:28professor of therapeutic radiology, member
• 00:30of the Yale Cancer Center,
• 00:32and vice chair for the
• 00:33physics research and education in
• 00:35therapeutic radiology.
• 00:36He has about three years
• 00:37of experience in radiation physics,
• 00:39and then he's an in
• 00:41house expert on gamma and
• 00:43X rays.
• 00:45He's also a fellow of
• 00:46the American Association of Physicists,
• 00:49in medicine and the chief
• 00:50physicist of Smile Local Hospital
• 00:52at Yale New Haven Hospital.
• 00:55So our
• 00:57mission of the shared resource
• 00:58irradiated shared resource is to
• 00:59provide easy and reliable access
• 01:01to cutting edge gamma and
• 01:02x-ray irradiation technologies,
• 01:04services, and scientific consultations
• 01:06for all the cancer researchers
• 01:08at Yale.
• 01:10So we have four objectives
• 01:12and aims. Aim one being
• 01:13providing convenient and reliable access
• 01:16to state of the art
• 01:17gamma ray and
• 01:18global digit, x-ray radiators with
• 01:20cost effective relation for all
• 01:22YCC members.
• 01:24We provide reliable access to
• 01:25all the radiators. We offer
• 01:26expert help with experimental design.
• 01:29We train all our users
• 01:30to operate the machines safely
• 01:32and keeping in line with
• 01:33EHS protocols,
• 01:34and we keep make sure
• 01:35we keep the machines running
• 01:36twenty four by seven.
• 01:41The key services of ISR,
• 01:42we provide radiation physics and
• 01:44technical consultation on the best
• 01:46use of all the radiators.
• 01:47We provide
• 01:48periodic quality assurance test to
• 01:50ensure accurate dose deliveries delivered
• 01:52by all the radiators.
• 01:53We train our users and
• 01:54help with compliance.
• 01:56We also escort users if
• 01:58they use a common radiator,
• 02:00then if they don't wanna
• 02:01go any background checks, which
• 02:02I'll talk about later.
• 02:04We also make sure we
• 02:05maintain our latest all year
• 02:06on.
• 02:07We do QH every month,
• 02:09every week, quarterly, and annually.
• 02:14So, we have, different equipment.
• 02:17We have a CCM gamma
• 02:19ray radiator.
• 02:20We have four precision x
• 02:22vat three twenty kilowatts irradiators
• 02:23across campus.
• 02:25We have our newest addition,
• 02:26the SARP, the small animal
• 02:28radiation research platform, MIDSGARRE accelerator,
• 02:31and we also have relation
• 02:32to equipment.
• 02:34Briefly,
• 02:35CGM one hundred and thirty
• 02:36seven gamma radiator provides,
• 02:38high penetration gamma rays,
• 02:40for whole body or cell
• 02:41variations.
• 02:42The Precision x-ray three twenty's,
• 02:44they deliver tunable kV x
• 02:46rays for cell tissue and
• 02:47small animal radiation with controlled
• 02:49dose rates. The SARP,
• 02:51enables a CT guided highly
• 02:53targeted radiation for precise small
• 02:55animal research.
• 02:56We also have radiation dosimeter
• 02:58equipment, which support both cell
• 03:00and animal studies.
• 03:02So, this is,
• 03:04this is how a gamma
• 03:05ray data looks like.
• 03:07It delivers up to six
• 03:09six and then six hundred
• 03:10sixty two kV gamma rays.
• 03:11It supports molecular samples, cell
• 03:13cancers, tumor cells, and small
• 03:14animals.
• 03:16It use it's also used
• 03:17for wide dose rate range
• 03:19and flexible collimation, and it
• 03:20has been a core tool
• 03:21for many years since nineteen
• 03:23ninety for all many YCC
• 03:24studies.
• 03:26We will be, removing this
• 03:27machine in the next year
• 03:28as part of a two
• 03:29program
• 03:30to play and replacing this
• 03:31with a new X-ray retail.
• 03:35So we have a four
• 03:37X-ray machines across campus.
• 03:40These are X-ray three twenties,
• 03:41made by Precision.
• 03:43They're used for rating cell
• 03:44cultures, beta layers, bacteria, insects,
• 03:46and small and small animals
• 03:47like rodents, mice, and rats.
• 03:49It's supposed both targeted and
• 03:51whole body radiation,
• 03:52used for studies in tumor
• 03:54and normal tissue response, hematopoietic
• 03:56depletion, immunotherapy relation with energy
• 03:59research.
• 03:59The output contain, it goes
• 04:01up to three grades per
• 04:02minute at fifty centimeters SSD,
• 04:04distributed across four medical campus
• 04:06buildings for community access.
• 04:08So we have four x-ray
• 04:09machines, one located in TAC,
• 04:11one located in Amistad, one
• 04:12located in George, and another
• 04:14one located in LCI building.
• 04:16And this is a gamma
• 04:16radiator.
• 04:19So our newest edition is
• 04:20called the SARP, small animal
• 04:22radiation research platform.
• 04:23It's an advanced multifunctional accelerator
• 04:26designed specifically for animal research.
• 04:28So what it is is
• 04:29a two twenty five kilowatt
• 04:30accelerator designed specifically for high
• 04:32precision irradiation of mice and
• 04:34rats.
• 04:35The key features of this
• 04:36machine would be cone beam
• 04:37CT imaging,
• 04:38a three d treatment planning
• 04:40just like a miniature clinical
• 04:41relation therapy planning system. It
• 04:43has a mobile gantry with
• 04:44a robotic stage so beams
• 04:46can come from many angles
• 04:47for precise targeting.
• 04:49It also has high sorry
• 04:51it has a high reproducibility.
• 04:55So why it matters, it
• 04:56it allows focal degradation of
• 04:58instead of whole body exposure
• 04:59so you could target precisely
• 05:01or what organ you wanna
• 05:02treat with.
• 05:03It ex reduces normal tissue
• 05:05injury by sparing healthy areas.
• 05:07It's essential for studying tumor
• 05:08response, immunology,
• 05:10DNA repair, and normal tissue
• 05:11toxicity.
• 05:12So in a simple summary,
• 05:13it's a clinical reason that
• 05:14we device, but scaled down
• 05:16for mice.
• 05:18So this is how it
• 05:19looks like.
• 05:23So the mouse goes on
• 05:24the bed over here. There's
• 05:26a CT detector.
• 05:27The camera takes a three
• 05:28d image.
• 05:30Your X-ray head is on
• 05:31the top.
• 05:32We have a collimator.
• 05:34You can choose your own.
• 05:35We have we have five
• 05:36different collimators of various tumor
• 05:37sizes. You can choose your
• 05:39collimator and,
• 05:41treat them, through the mouse
• 05:42or the rat with whatever
• 05:43tumor it has. So this
• 05:45is a treatment time software.
• 05:46Here, like, I show you
• 05:47a lung tumor,
• 05:49in the lung of a
• 05:50mouse so you can target
• 05:52just a tumor in the
• 05:53mouse.
• 05:56So we also have variation
• 05:57dosimetry equipment. We have INGMA
• 05:59based absolute dosimetry.
• 06:01It measures absolute dose and
• 06:03it converts collected charge into
• 06:05and converts that into a
• 06:06citric rate. It's used to
• 06:07calibrate our machines and we
• 06:09use it for daily and
• 06:10monthly
• 06:11QAs.
• 06:12It's sensitive to set up
• 06:13an environmental corrections.
• 06:15We also have a EBT4
• 06:16filament dosimetry. It provides two
• 06:18d dose distribution. It's great
• 06:20for checking uniformity, beam profiles,
• 06:22and small fields.
• 06:23It requires scanning and calibration
• 06:25curve. It goes for relative
• 06:27dose but not for the
• 06:27absolute dose.
• 06:29We also have a t
• 06:30l d dosimeter system.
• 06:32It's useful where films or
• 06:35ion chambers or gaphomic films,
• 06:37e b d four films
• 06:37can go and it's
• 06:40measures a point dose. It's
• 06:41good for verification of delivered
• 06:42dose. We also have a
• 06:44flaky chemical dosimeter.
• 06:46It measures radiation dose, but
• 06:47how much they how much
• 06:48it oxidizes ferrous ions in
• 06:50a specific sulfuric acid solution.
• 06:53So some of our experimental
• 06:55setups on gamma and actuators,
• 06:57we have a whole mouse
• 06:58pie, for mouse whole body
• 06:59radiation.
• 07:00We have shields available built
• 07:02for a brain radiation or
• 07:03a flying tumor radiation.
• 07:05You can always do tissue
• 07:07culture plates and culture flask
• 07:08for cell radiation.
• 07:11We also have two years
• 07:12filters for harder beams and
• 07:14aluminum two millimeter millimeter filter
• 07:15for a softer beam,
• 07:17so we can, always use
• 07:18those filters too.
• 07:22So, training and access
• 07:25is basically very simple. You
• 07:26have to do three three
• 07:27step three steps, get an
• 07:28online training with EHS, obtain
• 07:30a relation to submit it,
• 07:31fill out a form, and
• 07:31submit to EHS and a
• 07:32hands on training with me,
• 07:34and you would be approved
• 07:35to use,
• 07:36any of the exhibitions across
• 07:37campus.
• 07:40So same thing. Complete required
• 07:42acts required training and a
• 07:44plain relation to submit it.
• 07:45For a common radiator, you
• 07:46need to submit a background
• 07:47clearance form with with COVID
• 07:49with EHS. It takes about
• 07:51a while, but you don't
• 07:52have to wait for the
• 07:53background clearance. I can export
• 07:54escort you to the common
• 07:55radiator and perform your experiments
• 07:57for you.
• 08:00So all the scheduling and
• 08:01training is done via PPMS.
• 08:03I'll provide the link up
• 08:04here.
• 08:05Please access this link, and
• 08:06everything's up all all the
• 08:08information we need for training
• 08:09and scheduling is up on
• 08:10the PPMS website.
• 08:13So quickly,
• 08:14some of our research,
• 08:16here, we have a lung
• 08:17cancer presentation with doctor Hayman
• 08:19and doctor Contessa. So we
• 08:20use he they use the
• 08:21X-ray three twenty to deliver
• 08:22fractional doses for cell and
• 08:24mode studies.
• 08:25We had doctor Ryan Jensen
• 08:26for DNA repair. He used
• 08:28DNA x three twenty to
• 08:29induce DNA breaks to measure
• 08:31the RAD fifty one foci.
• 08:33We also have a humanized
• 08:34skeletal m model with doctor
• 08:35Flavell's lab.
• 08:36They use their sublethal expert
• 08:38conditioning
• 08:39for enable HSC engraftment in
• 08:41mice. And we also have
• 08:42doctor Pillai, from George billing.
• 08:44They use the x-ray three
• 08:45twenty to induce DNA damage
• 08:47to test synthetic lethal responses.
• 08:50So,
• 08:51for the sub basically of
• 08:52twenty twenty five, we had
• 08:54about fifty two PIs and
• 08:55twenty one departments that use
• 08:56our machines.
• 08:57Ninety eight percent of our
• 08:58members are YCC members. So,
• 09:01we we had a user
• 09:02of a thousand twelve two
• 09:03hundred for the fiscal year
• 09:04twenty twenty five.
• 09:07Quickly about the service rates,
• 09:08we charge about hundred and
• 09:09thirty dollars for thirty minutes.
• 09:11With assisted, that mean I
• 09:12would be doing operation for
• 09:13you. If you get trained
• 09:14and use it by yourself,
• 09:15it's sixty five dollars for
• 09:16thirty minutes. You don't need
• 09:17a you need just need
• 09:18training, and you have an
• 09:19underscore to access, meaning you
• 09:20can access the machine anytime
• 09:22area of the week.
• 09:25So this is our contact
• 09:26information here, doctor Jay Chen,
• 09:27and that's me, Kamalakram Maripuri.
• 09:29And our our website and
• 09:30people's website is included in
• 09:31the presentation over here, which
• 09:33I'll share with you in
• 09:33a few minutes. Thank you,
• 09:34everybody. Any questions?