Zachery Wolfe
Postdoctoral AssociateAbout
Research
Publications
2025
Deep transcriptomics reveals cell-specific isoforms of pan-neuronal genes
Wolfe Z, Liska D, Norris A. Deep transcriptomics reveals cell-specific isoforms of pan-neuronal genes. Nature Communications 2025, 16: 4507. PMID: 40379625, PMCID: PMC12084633, DOI: 10.1038/s41467-025-58296-2.Peer-Reviewed Original ResearchConceptsCell-specific isoformPan-neuronal genesSplicing patternsLowly-expressed genesCell-specific expression patternsPotential regulatory factorsIntron retentionDeep transcriptomeRNA-seqSplicing factorsAlternative splicingBiological replicatesGenetic interrogationSplicing mapsSplicingFactors in vivoSplice variantsGene expressionNeuron typesExpression patternsGenesLow capture efficiencyRegulatory factorsTranscriptomeIsoforms
2023
VISTA: visualizing the spatial transcriptome of the C.elegans nervous system
Liska D, Wolfe Z, Norris A. VISTA: visualizing the spatial transcriptome of the C.elegans nervous system. Bioinformatics Advances 2023, 3: vbad127. PMID: 37810458, PMCID: PMC10560093, DOI: 10.1093/bioadv/vbad127.Peer-Reviewed Original ResearchSingle-cell resolutionSpatial transcriptomicsTranscriptomes of single cellsExpression patternsNematode worm Caenorhabditis elegans isNeuron-specific gene expressionInvariant cell lineageCaenorhabditis elegansGene expression valuesGene expression patternsAnimal speciesQuery geneCellular identityTranscriptomeReporter geneGene expressionGenesExpression valuesCell lineagesIndividual cellsExpression levelsSingle cellsSpeciesExpressionNervous system
2022
Microbe-mediated activation of indoxacarb in German cockroach (Blattella germanica L.)
Wolfe Z, Scharf M. Microbe-mediated activation of indoxacarb in German cockroach (Blattella germanica L.). Pesticide Biochemistry And Physiology 2022, 188: 105234. PMID: 36464351, DOI: 10.1016/j.pestbp.2022.105234.Peer-Reviewed Original ResearchConceptsGerman cockroachCockroach gutSusceptible laboratory strainExposure to insecticidesGerman cockroach populationsHydrolase activity assaysInsecticide treatmentsFeeding bioassaysSusceptible cockroachesCockroach strainsPest worldwideBait productsControl treatmentPro-insecticideIndoxacarbFrass extractsField populationsGeneralist feeding habitsCockroach populationsFeeding habitsInsecticidesLaboratory strainsPotentially hazardous substancesHost insectsDetoxification capabilitiesTranscriptome Responses to Defined Insecticide Selection Pressures in the German Cockroach (Blattella germanica L.)
Scharf M, Wolfe Z, Raje K, Fardisi M, Thimmapuram J, Bhide K, Gondhalekar A. Transcriptome Responses to Defined Insecticide Selection Pressures in the German Cockroach (Blattella germanica L.). Frontiers In Physiology 2022, 12: 816675. PMID: 35185605, PMCID: PMC8856671, DOI: 10.3389/fphys.2021.816675.Peer-Reviewed Original ResearchInsecticide selection pressureInsecticide resistanceInsecticide resistance evolutionGenerations of selectionGerman cockroachUpregulated DE genesDE genesDetoxification enzyme familiesGlutathione-S -transferaseIndoxacarb baitInsecticide productsParent stockStress response mechanismsEffectiveness of antimicrobial preservativesInsecticidesBait matrixControl linesSelection pressureResistance evolutionCockroach controlUrban pestsBaitActive ingredientsTranscriptomic responseGeneral stress response mechanisms
2021
Author Correction: Differential microbial responses to antibiotic treatments by insecticide-resistant and susceptible cockroach strains (Blattella germanica L.)
Wolfe Z, Scharf M. Author Correction: Differential microbial responses to antibiotic treatments by insecticide-resistant and susceptible cockroach strains (Blattella germanica L.). Scientific Reports 2021, 11: 24536. PMID: 34969950, PMCID: PMC8718527, DOI: 10.1038/s41598-021-04470-7.Peer-Reviewed Original ResearchDifferential microbial responses to antibiotic treatments by insecticide-resistant and susceptible cockroach strains (Blattella germanica L.)
Wolfe Z, Scharf M. Differential microbial responses to antibiotic treatments by insecticide-resistant and susceptible cockroach strains (Blattella germanica L.). Scientific Reports 2021, 11: 24196. PMID: 34921232, PMCID: PMC8683489, DOI: 10.1038/s41598-021-03695-w.Peer-Reviewed Original ResearchConceptsInsecticide-resistantSusceptible strainsLC50 resistance ratiosGerman cockroachDoses of abamectinGut bacteriaMicrobial compositionInsecticides abamectinCockroach strainsPest worldwideControl dietGut microbial compositionInsecticide resistanceAbsence of antibioticsBacterial taxaCockroach gutAbamectinGut microbiomeFipronilInsecticidesResistance ratioDietWhole gutBacterial DNAKanamycin treatment