Zachery Wolfe
Postdoctoral AssociateAbout
Research
Publications
2025
Deep transcriptomics reveals cell-specific isoforms of pan-neuronal genes
Wolfe Z, Liska D, Norris A. Deep transcriptomics reveals cell-specific isoforms of pan-neuronal genes. Nature Communications 2025, 16: 4507. PMID: 40379625, PMCID: PMC12084633, DOI: 10.1038/s41467-025-58296-2.Peer-Reviewed Original ResearchConceptsCell-specific isoformPan-neuronal genesSplicing patternsLowly-expressed genesCell-specific expression patternsPotential regulatory factorsIntron retentionDeep transcriptomeRNA-seqSplicing factorsAlternative splicingBiological replicatesGenetic interrogationSplicing mapsSplicingFactors in vivoSplice variantsGene expressionNeuron typesExpression patternsGenesLow capture efficiencyRegulatory factorsTranscriptomeIsoforms
2023
VISTA: visualizing the spatial transcriptome of the C.elegans nervous system
Liska D, Wolfe Z, Norris A. VISTA: visualizing the spatial transcriptome of the C.elegans nervous system. Bioinformatics Advances 2023, 3: vbad127. PMID: 37810458, PMCID: PMC10560093, DOI: 10.1093/bioadv/vbad127.Peer-Reviewed Original ResearchSingle-cell resolutionSpatial transcriptomicsTranscriptomes of single cellsExpression patternsNematode worm Caenorhabditis elegans isNeuron-specific gene expressionInvariant cell lineageCaenorhabditis elegansGene expression valuesGene expression patternsAnimal speciesQuery geneCellular identityTranscriptomeReporter geneGene expressionGenesExpression valuesCell lineagesIndividual cellsExpression levelsSingle cellsSpeciesExpressionNervous system