Wenwen Tang, PhD
Research Scientist in PharmacologyCards
About
Research
Publications
2025
MMP9High Neutrophils are Critical Mediators of Neutrophil Extracellular Traps Formation and Myocardial Ischemia/Reperfusion Injury
Hu S, Zhang F, Wang J, Zhang J, Li C, Lyu Y, Wang Y, Huang R, Gao Y, Yang H, Qian J, Tang W, Cao J, Ge J. MMP9High Neutrophils are Critical Mediators of Neutrophil Extracellular Traps Formation and Myocardial Ischemia/Reperfusion Injury. Advanced Science 2025, 12: 2415205. PMID: 40151877, PMCID: PMC12140383, DOI: 10.1002/advs.202415205.Peer-Reviewed Original ResearchConceptsNeutrophil extracellular trap formationNeutrophil extracellular trapsMyocardial ischemia/reperfusion injuryIschemia/reperfusion injuryBlood of healthy donorsNeutrophil-mediated reperfusion injuryTherapeutic targetExtracellular trap formationTranscription factor SPI1Potential therapeutic targetHealthy donors
2024
Single-cell RNA-seq analysis of cell-cell communications in human lung reveals a novel role of VEGF-D in acute lung injury
Yuan Y, Sharma L, Tang W, Raredon M, Ahangari F, Khoury J, Wu D, Niklason L, Kaminski N. Single-cell RNA-seq analysis of cell-cell communications in human lung reveals a novel role of VEGF-D in acute lung injury. Physiology 2024, 39: 1314. DOI: 10.1152/physiol.2024.39.s1.1314.Peer-Reviewed Original ResearchIdiopathic pulmonary fibrosisAcute lung injuryChronic obstructive pulmonary diseaseAcute respiratory distress syndromeAnalysis of cell-cell communicationVEGF-DMicrovascular nicheSingle-cell RNA-seqLung injury modelSingle-cell RNA-seq analysisLung injuryCell-cell communicationLigand-receptor pairsLPS-induced lung injury modelRNA-seqAdjacent cell typesPulmonary diseaseInjury modelHuman lung endothelial cellsBarrier functionImmune cell infiltrationTumor necrosis factor-aRespiratory distress syndromeLung vascular integrityGene expressionVEGF-D Is Enriched in the Human Lung Microvascular Niche and Improves Vascular Integrity During Acute Lung Injury
Yuan Y, Sharma L, Tang W, Raredon M, Ahangari F, Khoury J, Wu D, Niklason L, Kaminski N. VEGF-D Is Enriched in the Human Lung Microvascular Niche and Improves Vascular Integrity During Acute Lung Injury. 2024, a4694-a4694. DOI: 10.1164/ajrccm-conference.2024.209.1_meetingabstracts.a4694.Peer-Reviewed Original ResearchLDL receptor-related protein 5 selectively transports unesterified polyunsaturated fatty acids to intracellular compartments
Tang W, Luan Y, Yuan Q, Li A, Chen S, Menacherry S, Young L, Wu D. LDL receptor-related protein 5 selectively transports unesterified polyunsaturated fatty acids to intracellular compartments. Nature Communications 2024, 15: 3068. PMID: 38594269, PMCID: PMC11004178, DOI: 10.1038/s41467-024-47262-z.Peer-Reviewed Original ResearchConceptsLDL receptor-related protein 5Intracellular compartmentsPolyunsaturated fatty acidsProtein 5Ligand-binding repeatsBiologically important mechanismsAssociated with human healthImport mechanismExtracellular trap formationInhibit mTORC1Cell typesFatty acidsTrap formationN-3 polyunsaturated fatty acidsCompartmentProtect miceLDLAMyocardial injuryHomologyIschemia-reperfusionMTORC1LysosomesLRP6Human healthFATP2Wnt5 controls splenic myelopoiesis and neutrophil functional ambivalency during DSS-induced colitis
Luan Y, Hu J, Wang Q, Wang X, Li W, Qu R, Yang C, Rajendran B, Zhou H, Liu P, Zhang N, Shi Y, Liu Y, Tang W, Lu J, Wu D. Wnt5 controls splenic myelopoiesis and neutrophil functional ambivalency during DSS-induced colitis. Cell Reports 2024, 43: 113934. PMID: 38461416, PMCID: PMC11064424, DOI: 10.1016/j.celrep.2024.113934.Peer-Reviewed Original ResearchCD8<sup>+</sup> T cell activationNeutrophil productionNeutrophil plasticitySplenic extramedullary myelopoiesisFamily member 5T cell activationInnate immune cellsSplenic stromal cellsDSS-induced colitisAnti-inflammatory protectionCD101 expressionPro-inflammatory activitySplenic myelopoiesisExtramedullary myelopoiesisBone marrowImmune cellsSplenic neutrophilsMember 5Autoimmune diseasesInflammatory outcomesCell activationStromal cellsColitisSplenic productionElevated numbersCell surface RNAs control neutrophil recruitment
Zhang N, Tang W, Torres L, Wang X, Ajaj Y, Zhu L, Luan Y, Zhou H, Wang Y, Zhang D, Kurbatov V, Khan S, Kumar P, Hidalgo A, Wu D, Lu J. Cell surface RNAs control neutrophil recruitment. Cell 2024, 187: 846-860.e17. PMID: 38262409, PMCID: PMC10922858, DOI: 10.1016/j.cell.2023.12.033.Peer-Reviewed Original ResearchConceptsCell surfaceMammalian homologOuter cell surfaceRNA transportGlycan modificationsMammalian cellsSID-1Cellular functionsRecruitment to inflammatory sitesGlycoRNARNAMurine neutrophilsFunctional significanceNeutrophil recruitmentNeutrophil recruitment to inflammatory sitesBiological importanceCellsNeutrophil adhesionReduced neutrophil adhesionHomologyGlycansGenesInflammatory sitesRecruitmentEndothelial cellsThe CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8+ T cells
Liao X, Li W, Zhou H, Rajendran B, Li A, Ren J, Luan Y, Calderwood D, Turk B, Tang W, Liu Y, Wu D. The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8+ T cells. Nature Communications 2024, 15: 603. PMID: 38242867, PMCID: PMC10798966, DOI: 10.1038/s41467-024-44885-0.Peer-Reviewed Original ResearchConceptsCD8+ T cellsT cellsCancer immunotherapyMouse CD8+ T cellsAnti-tumor immunityTumor growth inhibition abilityAnti-tumor effectsInhibition of neddylationCD8Effector functionsTCR stimulationIL2 signalingCentral signaling pathwaysCore signaling pathwaysEffector activityNegative regulatory mechanismsTranslational implicationsImmunotherapyGrowth inhibition abilityCytokine signalingTCRProteomic alterationsSignaling pathwayCancerCRISPR-based screens
2023
Cell Surface RNAs Control Neutrophil Function
Zhang N, Tang W, Torres L, Zhu L, Wang X, Ajaj Y, Wang Y, Zhang D, Kurbatov V, Zhou H, Luan Y, Kumar P, Hidalgo A, Wu D, Lu J. Cell Surface RNAs Control Neutrophil Function. Blood 2023, 142: 674. DOI: 10.1182/blood-2023-187570.Peer-Reviewed Original ResearchExtracellular RNaseCell surfaceTotal RNABona fide ligandsEndothelial cellsOuter cell surfaceTransendothelial migrationMammalian cellsSuch RNAsGlycan modificationsCellular RNAGlycoRNARNase digestionLive cellsRNAHematopoietic cellsRNase treatmentSimilar defectsIntegrin levelsConfocal microscopyRNaseGlycan fractionImportant functionsHomologuesRecombinant E-selectinSfxn5 Regulation of Actin Polymerization for Neutrophil Spreading Depends on a Citrate-Cholesterol-PI(4,5)P2 Pathway.
Zhang H, Meng L, Liu Y, Jiang J, He Z, Qin J, Wang C, Yang M, He K, Yang J, Chen K, He Q, Tang W, Fan S, Ren C. Sfxn5 Regulation of Actin Polymerization for Neutrophil Spreading Depends on a Citrate-Cholesterol-PI(4,5)P2 Pathway. The Journal Of Immunology 2023, 211: 462-473. PMID: 37326485, DOI: 10.4049/jimmunol.2200863.Peer-Reviewed Original ResearchConceptsRegulation of actin polymerizationActin polymerizationNeutrophil spreadingCell spreadingLevels of phosphatidylinositol 4,5-bisphosphatePromote actin polymerizationNeutrophil actin polymerizationPhysiological cellular functionsPhosphatidylinositol 4,5-bisphosphateCytosolic citrateSmall interfering RNA transfectionFamily proteinsSFXN5Acetyl-CoAPI(4,5)P2 levelsMitochondrial membraneSpreading neutrophilsMetabolite transportCellular functionsMorpholino injectionCellular phenotypesCitrate transporterPlasma membraneDownstream metabolic productsDecreased neutrophil recruitment
2022
SARS-CoV-2 Continuous Genetic Divergence and Changes in Multiplex RT-PCR Detection Pattern on Positive Retesting Median 150 Days after Initial Infection
Liu D, Rodriguez G, Zhou H, Cheng Y, Li X, Tang W, Prasad N, Chen C, Singh V, Konadu E, James K, Bahamon M, Chen Y, Segal-Maurer S, Wu A, Rodgers W. SARS-CoV-2 Continuous Genetic Divergence and Changes in Multiplex RT-PCR Detection Pattern on Positive Retesting Median 150 Days after Initial Infection. International Journal Of Molecular Sciences 2022, 23: 6254. PMID: 35682933, PMCID: PMC9181733, DOI: 10.3390/ijms23116254.Peer-Reviewed Original ResearchConceptsWhole genome amplificationNext generation sequencingGenetic divergenceGene ampliconsMRT-PCRViral genomeMolecular evolutionGenome amplificationMutational hotspotsGeneration sequencingAmplification-based assaysInitial infectionGene amplification assaysMultiplex reverse transcription polymerase chain reactionMutation frequencyMutationsDetect patternsGenomeSARS-CoV-2AmpliconsPolymerase chain reactionAmplification assayDivergenceReverse transcription polymerase chain reactionTranscription polymerase chain reaction
Get In Touch
Contacts
Email