Sunandini Chandra, PhD
Cards
About
Research
Publications
2022
Emerging Connections between Nuclear Pore Complex Homeostasis and ALS
Chandra S, Lusk CP. Emerging Connections between Nuclear Pore Complex Homeostasis and ALS. International Journal Of Molecular Sciences 2022, 23: 1329. PMID: 35163252, PMCID: PMC8835831, DOI: 10.3390/ijms23031329.Peer-Reviewed Original ResearchConceptsNuclear pore complexNuclear envelopeQuality control pathwaysNuclear transport machineryNuclear transport receptorsQuality control mechanismsDipeptide repeat proteinsNew experimental avenuesNucleoporin proteinsRan GTPaseAmyotrophic lateral sclerosisTransport machineryPore complexRepeat proteinsSurveillance pathwayTransport receptorsRepeat RNANPC structureTransport proteinsControl pathwaysPatient neuronsComplex homeostasisHexanucleotide repeat expansionExperimental avenuesRepeat expansion
2021
Atg39 selectively captures inner nuclear membrane into lumenal vesicles for delivery to the autophagosome
Chandra S, Mannino PJ, Thaller DJ, Ader NR, King MC, Melia TJ, Lusk CP. Atg39 selectively captures inner nuclear membrane into lumenal vesicles for delivery to the autophagosome. Journal Of Cell Biology 2021, 220: e202103030. PMID: 34714326, PMCID: PMC8575018, DOI: 10.1083/jcb.202103030.Peer-Reviewed Original ResearchMeSH KeywordsAutophagosomesAutophagyAutophagy-Related ProteinsCytoplasmic VesiclesGreen Fluorescent ProteinsNuclear EnvelopeProtein DomainsReceptors, Cytoplasmic and NuclearSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsStructure-Activity RelationshipTime FactorsVacuolesVesicular Transport ProteinsConceptsInner nuclear membraneNuclear envelope lumenOuter nuclear membraneNuclear membraneSplit-GFP reporterNuclear envelope localizationINM proteinsAutophagy apparatusEnvelope localizationLumenal vesiclesLumenal domainCargo adaptorsAtg39Sequence elementsCorrelative lightVesiclesAutophagosomesMembraneNucleophagyAdaptorReporterProteinOverexpressionMotif
2020
Ectopic expression of 35 kDa and knocking down of 78 kDa SG2NAs induce cytoskeletal reorganization, alter membrane sialylation, and modulate the markers of EMT
Gupta R, Kumar G, Jain B, Chandra S, Goswami S. Ectopic expression of 35 kDa and knocking down of 78 kDa SG2NAs induce cytoskeletal reorganization, alter membrane sialylation, and modulate the markers of EMT. Molecular And Cellular Biochemistry 2020, 476: 633-648. PMID: 33083950, DOI: 10.1007/s11010-020-03932-2.Peer-Reviewed Original Research
2019
Site‐specific phosphorylation of villin remodels the actin cytoskeleton to regulate Sendai viral glycoprotein‐mediated membrane fusion
Chandra S, Kumar M, Sharma N, Sarkar D. Site‐specific phosphorylation of villin remodels the actin cytoskeleton to regulate Sendai viral glycoprotein‐mediated membrane fusion. FEBS Letters 2019, 593: 1927-1943. PMID: 31183850, DOI: 10.1002/1873-3468.13477.Peer-Reviewed Original ResearchConceptsMembrane fusionVirus-host cell membrane fusionKey phosphorylation sitesQuantitative mass spectrometrySite-specific phosphorylationCell membrane fusionChinese hamster ovary cellsActin cytoskeletonPhosphorylation sitesHamster ovary cellsC-SrcTyrosine phosphorylationDependent phosphorylationCellular factorsCell fusionPhosphorylationOvary cellsVillinCritical roleVillin expressionSendai virosomesMass spectrometryFusionImportant roleCytoskeleton
2018
A combinatorial approach for robust transgene delivery and targeted expression in mammary gland for generating biotherapeutics in milk, bypassing germline gene integration
Ganguli N, Ganguli N, Chandra S, Choubey M, Sarkar D, Majumdar S. A combinatorial approach for robust transgene delivery and targeted expression in mammary gland for generating biotherapeutics in milk, bypassing germline gene integration. Applied Microbiology And Biotechnology 2018, 102: 6221-6234. PMID: 29855689, DOI: 10.1007/s00253-018-9094-2.Peer-Reviewed Original ResearchConceptsΒ-casein promoterGene integrationHemagglutinin-neuraminidaseEpithelial cell-specific expressionSpecific gene expressionCell-specific expressionMammary epithelial cellsEpithelial cellsNative genomeAnimal bioreactorsMammary glandMembrane fusionGene expressionSpecific expressionTransgene constructLuminal epithelial cellsMilk glandTargeted expressionViral membraneTransgeneFarmed animalsProtein expressionTherapeutic proteinsPromoterFusion factor
2017
Sendai virus recruits cellular villin to remodel actin cytoskeleton during fusion with hepatocytes
Chandra S, Kalaivani R, Kumar M, Srinivasan N, Sarkar D. Sendai virus recruits cellular villin to remodel actin cytoskeleton during fusion with hepatocytes. Molecular Biology Of The Cell 2017, 28: 3801-3814. PMID: 29074568, PMCID: PMC5739296, DOI: 10.1091/mbc.e17-06-0400.Peer-Reviewed Original ResearchConceptsMembrane fusionQuantitative mass spectrometryTwo-dimensional differentialViral envelope glycoproteinsSendai viral envelopesEarly molecular eventsHost cell proteinsActin cytoskeletonThreonine 206Actin dynamicsSendai virusAnimal cellsMolecular eventsCellular membranesHost cellsCell proteinsFusion-mediated deliveryNovel mechanismBiochemical analysisVillinEnvelope glycoproteinGel electrophoresisViral envelopeProteinViral entry
2015
Phosphorylation of Nonmuscle myosin II-A regulatory light chain resists Sendai virus fusion with host cells
Das P, Saha S, Chandra S, Das A, Dey S, Das M, Sen S, Sarkar D, Jana S. Phosphorylation of Nonmuscle myosin II-A regulatory light chain resists Sendai virus fusion with host cells. Scientific Reports 2015, 5: 10395. PMID: 25993465, PMCID: PMC4438666, DOI: 10.1038/srep10395.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsCell Line, TumorCHO CellsCricetinaeCricetulusHeterocyclic Compounds, 4 or More RingsHumansMicroscopy, Atomic ForceMicroscopy, FluorescenceMutagenesisMyosin Light ChainsNonmuscle Myosin Type IIANonmuscle Myosin Type IIBPhosphorylationRho-Associated KinasesRNA InterferenceRNA, Small InterferingSendai virusSequence AlignmentVirus InternalizationVirus ReleaseMembrane Fusion Mediated Targeted Cytosolic Drug Delivery Through scFv Engineered Sendai Viral Envelopes.
Kumar M, Mani P, Pratheesh P, Chandra S, Jeyakkodi M, Chattopadhyay P, Sarkar D, Sinha S. Membrane Fusion Mediated Targeted Cytosolic Drug Delivery Through scFv Engineered Sendai Viral Envelopes. 2015, 15: 386-400. PMID: 25941820, DOI: 10.2174/1566524015666150505155949.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAnimalsAntibiotics, AntineoplasticAntibodies, MonoclonalCell Line, TumorCHO CellsCricetinaeCricetulusCytoplasmDoxorubicinDrug Delivery SystemsFluorescein-5-isothiocyanateGPI-Linked ProteinsHeLa CellsHumansIsoenzymesMembrane FusionPeptide LibrarySendai virusStaining and LabelingViral Envelope ProteinsViral Fusion ProteinsConceptsOnco-fetal antigenF proteinNumber of malignanciesNative F proteinVariety of cancersAlkaline phosphataseViral envelopeCytoplasmic deliveryBreast cancerSendai viral envelopesSpecific antibodiesPAPPlacental isozymeCancerSpecific bindingAntibodiesDrugsDeliveryCytosolic drug deliveryPotential usefulnessRecombinant scFvReduced degradationHN proteinUbiquitous expression
2011
Monocyte chemotactic protein (MCP3) promoter polymorphism is associated with atopic asthma in the Indian population
Batra J, Das S, Chatterjee R, Chandra S, Ghosh B. Monocyte chemotactic protein (MCP3) promoter polymorphism is associated with atopic asthma in the Indian population. Journal Of Allergy And Clinical Immunology 2011, 128: 239-242.e3. PMID: 21388664, DOI: 10.1016/j.jaci.2011.01.044.Peer-Reviewed Original Research
2010
Glycobiology of the Leishmania parasite and emerging targets for antileishmanial drug discovery
Chandra S, Ruhela D, Deb A, Vishwakarma R. Glycobiology of the Leishmania parasite and emerging targets for antileishmanial drug discovery. Expert Opinion On Therapeutic Targets 2010, 14: 739-757. PMID: 20536412, DOI: 10.1517/14728222.2010.495125.Peer-Reviewed Original ResearchConceptsDrug targetsUpcoming drug targetsNew drug targetsPurine salvage pathwayDrug discoveryPromising drug targetSalvage pathwayNovel pathwayRelated biochemistryAnchored glycoconjugatesLeishmania speciesPathwayDrug resistanceLeishmania parasitesAntileishmanial drug discoveryDiscoveryGlycosomesTargetGenomicsGlycosylphosphatidylinositolGlycobiologySpeciesBioinformaticsParasitic diseasesProtozoa