Sreya Das, PhD
Associate Research ScientistAbout
Research
Publications
2026
Dual recruitment of two CCM2 molecules to KRIT1 suppresses KLF4 expression
Huet-Calderwood C, Fisher O, Das S, Su V, Boggon T, Calderwood D. Dual recruitment of two CCM2 molecules to KRIT1 suppresses KLF4 expression. Nature Communications 2026, 17: 2719. PMID: 41688454, PMCID: PMC13013565, DOI: 10.1038/s41467-026-69595-7.Peer-Reviewed Original ResearchConceptsCerebral cavernous malformation 2PTB domainTranscription factorsMaintenance of endothelial cellsNormal regulationPartner proteinsKLF4 expressionPurified proteinCo-ImmunoprecipitationBiophysical analysisOver-expressionKLF4 proteinCerebral cavernous malformationsProteinCell linesK-RevEndothelial cell lineKLF4 transcription factorsCo-crystallographyExpressionProtein bindingKLF4MotifEndothelial cellsKnockdown
2025
Genetic and oncogenic features of RASGRF fusions
Das S, Lenchner D, Jaeger E, Hunihan L, DeSantis D, Fragkogianni S, Ronski K, Wilson F. Genetic and oncogenic features of RASGRF fusions. Npj Precision Oncology 2025, 9: 224. PMID: 40615519, PMCID: PMC12227644, DOI: 10.1038/s41698-025-01017-1.Peer-Reviewed Original ResearchGuanine exchange factorDH domainRNA-seq dataNon-small cell lung carcinomaExchange factorGenetic heterogeneityMembrane localizationCellular transformationChimeric proteinDiverse human malignanciesPH1 domainRas activationMAPK pathway inhibitionTransforming activityOncogenic featuresSubsets of cancersRasGRFOncogenic mechanismsFunctional assaysTargeted therapeutic strategiesRasGRF1Human malignanciesStructural diversityOncogenic fusionsPathway inhibition
2021
IL-9 Abrogates the Metastatic Potential of Breast Cancer by Controlling Extracellular Matrix Remodeling and Cellular Contractility.
Das S, Surve V, Marathe S, Wad S, Karulkar A, Srinivasan S, Dwivedi A, Barthel S, Purwar R. IL-9 Abrogates the Metastatic Potential of Breast Cancer by Controlling Extracellular Matrix Remodeling and Cellular Contractility. The Journal Of Immunology 2021, 206: 2740-2752. PMID: 34021045, DOI: 10.4049/jimmunol.2000383.Peer-Reviewed Original ResearchConceptsControlling extracellular matrix remodelingExtracellular matrix remodelingCellular contractilityIncreased focal adhesion formationFocal adhesion dynamicsFocal adhesion formationCancer cellsThree-dimensional invasion assayMatrix remodelingInvolvement of IL-9Metastatic potential of breastMyosin L chainSuppression of invasionCervical cancer cellsActomyosin contractilityCancer cell metastasisMDA-MB-231IL-9Metastatic potentialAdhesion dynamicsActivity of IL-9Human breastReduced phosphorylationInvasion assayCD8+ T cellsMultiomics Analysis and Systems Biology Integration Identifies the Roles of IL-9 in Keratinocyte Metabolic Reprogramming
Marathe S, Dhamija B, Kumar S, Jain N, Ghosh S, Dharikar J, Srinivasan S, Das S, Sawant A, Desai S, Khan F, Syiemlieh A, Munde M, Nayak C, Gandhi M, Kumar A, Srivastava S, Venkatesh K, Barthel S, Purwar R. Multiomics Analysis and Systems Biology Integration Identifies the Roles of IL-9 in Keratinocyte Metabolic Reprogramming. Journal Of Investigative Dermatology 2021, 141: 1932-1942. PMID: 33667432, DOI: 10.1016/j.jid.2021.02.013.Peer-Reviewed Original ResearchConceptsMetabolic reprogrammingHuman primary keratinocytesIL-9Production of tricarboxylic acid cycle intermediatesRedirection of metabolic fluxIntegration of multiomics dataCentral carbohydrate metabolismElectron transport chain proteinsPrimary keratinocytesHigh-throughput quantitative proteomicsTricarboxylic acid cycle intermediatesStable isotope metabolomicsNuclear magnetic resonance-based metabolomicsElectron transport chainSystems-level analysisIL-9-producing T cellsPathogenesis of inflammatory disordersMetabolic fluxIncreased glucose consumptionIL-9 signalingTransport chainQuantitative proteomicsMultiomics dataCycle intermediatesMultiomics analysis
2019
Blockade of ROCK inhibits migration of human primary keratinocytes and malignant epithelial skin cells by regulating actomyosin contractility
Srinivasan S, Das S, Surve V, Srivastava A, Kumar S, Jain N, Sawant A, Nayak C, Purwar R. Blockade of ROCK inhibits migration of human primary keratinocytes and malignant epithelial skin cells by regulating actomyosin contractility. Scientific Reports 2019, 9: 19930. PMID: 31882703, PMCID: PMC6934852, DOI: 10.1038/s41598-019-56447-2.Peer-Reviewed Original ResearchConceptsRho-Associated Protein KinaseMyosin light chain kinaseRegulates actomyosin contractilityMyosin light chainA-431 cellsActomyosin contractilityFocal adhesionsA-431Loss of migrationPhosphorylation of myosin light chainPhosphorylated myosin light chainPrimary keratinocytesLight chain kinaseMyosin light chain kinase expressionStress fibresCarcinoma cell linesProtein kinaseHuman primary keratinocytesSkin epithelial cellsKinaseMalignant skin cellsEpidermal carcinoma cell lineSkin cellsEpithelial skin cellsPhysiological processesHydrogel scaffold with substrate elasticity mimicking physiological-niche promotes proliferation of functional keratinocytes
Mogha P, Srivastava A, Kumar S, Das S, Kureel S, Dwivedi A, Karulkar A, Jain N, Sawant A, Nayak C, Majumder A, Purwar R. Hydrogel scaffold with substrate elasticity mimicking physiological-niche promotes proliferation of functional keratinocytes. RSC Advances 2019, 9: 10174-10183. PMID: 31304009, PMCID: PMC6592153, DOI: 10.1039/c9ra00781d.Peer-Reviewed Original ResearchSkin disordersHydrogel scaffoldsInert hydrogelSubstrate elasticityGene delivery efficiencyFunctional keratinocytesHydrogelsMechanical interactionTherapy of skin disordersGene therapyHuman primary keratinocytesAutologous graftsPrimary keratinocytesRegenerative capacityAccelerated proliferationFeeder layerWound healing assaySkin elasticityDelivery efficiencyNon-cytotoxicKeratinocytesElasticityDelivery systemDifferential Influence of IL-9 and IL-17 on Actin Cytoskeleton Regulates the Migration Potential of Human Keratinocytes
Das S, Srinivasan S, Srivastava A, Kumar S, Das G, Das S, Dwivedi A, Karulkar A, Makkad K, Bilala R, Gupta A, Sawant A, Nayak C, Tayalia P, Purwar R. Differential Influence of IL-9 and IL-17 on Actin Cytoskeleton Regulates the Migration Potential of Human Keratinocytes. The Journal Of Immunology 2019, 202: 1949-1961. PMID: 30760620, DOI: 10.4049/jimmunol.1800823.Peer-Reviewed Original ResearchConceptsActin stress fibersHuman primary keratinocytesActomyosin machineryActomyosin cytoskeletonActin cytoskeletonCytoskeleton reorganizationStress fibersIncreased migration potentialMigration potentialRegulates numerous functionsMyosin L chainA-431 cellsHuman keratinocytesCellular contractilityProteolytic cleavageCytoskeletonSecreting specific cytokinesActomyosinIL-9Skin immune surveillanceActinMMP-independent mannerT cell cytokinesKeratinocyte migrationSkin homeostasis
2017
Blockade of Rho-associated protein kinase (ROCK) inhibits the contractility and invasion potential of cancer stem like cells
Srinivasan S, Ashok V, Mohanty S, Das A, Das S, Kumar S, Sen S, Purwar R. Blockade of Rho-associated protein kinase (ROCK) inhibits the contractility and invasion potential of cancer stem like cells. Oncotarget 2017, 5: 21418-21428. PMID: 28199964, PMCID: PMC5400594, DOI: 10.18632/oncotarget.15248.Peer-Reviewed Original ResearchConceptsCancer stem like cellsRho-Associated Protein KinaseProtein kinaseInvasive potentialCancer metastasisCell invasive potentialBreast cancer cell linesCancer cell linesTreatment of cancer metastasisNon-CSCsCollagen degradation assayPrevention of cancer metastasisCell contractilityRegulating invasionCell invasionMolecular mechanismsDe-adhesionDegradation assayCell linesROCK pathwayCellular levelKinaseCellsPharmacological targetsDegradation capacity
2015
Reversible induction of translational isoforms of p53 in glucose deprivation
Khan D, Katoch A, Das A, Sharathchandra A, Lal R, Roy P, Das S, Chattopadhyay S, Das S. Reversible induction of translational isoforms of p53 in glucose deprivation. Cell Death & Differentiation 2015, 22: 1203-1218. PMID: 25721046, PMCID: PMC4572868, DOI: 10.1038/cdd.2014.220.Peer-Reviewed Original ResearchConceptsIRES activityP53 isoformsMaster transcription regulatorTumor suppressor protein p53Bicistronic reporter constructCritical tumor suppressorSuppressor protein p53Glucose deprivationFull-length p53P53 IRES activityP53 mRNACell cycle arrestTranscription regulatorsNutrient starvationTranslational regulationEndoplasmic reticulum stressDifferent stress conditionsNuclear proteinsInternal ribosome entry site (IRES) structureGlucose starvationTarget genesCellular pathwaysStarvation-induced increaseReporter constructsTumor suppressor
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