Lei Lei
Associate Research ScientistCards
About
Research
Publications
2024
Do pulmonary ionocytes absorb chloride or secrete chloride?
Thornell I, Lei L, McCray P, Welsh M. Do pulmonary ionocytes absorb chloride or secrete chloride? American Journal Of Physiology - Cell Physiology 2024, 328: c400-c403. PMID: 39716827, PMCID: PMC12186486, DOI: 10.1152/ajpcell.00672.2024.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorPulmonary ionocyteLevels of cystic fibrosis transmembrane conductance regulatorShort-circuit current techniqueTransmembrane conductance regulatorTransepithelial voltageConductance regulatorVoltage clampSecrete chlorideSecretionResultant movementsIonocytesTransepithelialPhysiological conditionsIl-13 induces loss of CFTR in ionocytes and reduces airway epithelial fluid absorption
Ibarra G, Lei L, Yu W, Thurman A, Gansemer N, Meyerholz D, Pezzulo A, McCray P, Thornell I, Stoltz D. Il-13 induces loss of CFTR in ionocytes and reduces airway epithelial fluid absorption. Journal Of Clinical Investigation 2024, 134: e181995. PMID: 39255033, PMCID: PMC11527443, DOI: 10.1172/jci181995.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorLoss of cystic fibrosis transmembrane conductance regulatorAirway surface liquidIL-13 treatmentIL-13Liquid secretionAirway diseaseApical cystic fibrosis transmembrane conductance regulatorAirway surface liquid volumeAirway epithelial cell typesApical CFTR channelsHuman airway epitheliaEffects of IL-13Associated with airflow obstructionTransmembrane conductance regulatorGoblet cell metaplasiaChronic obstructive pulmonary diseaseIL-4/IL-13Lung defense mechanismsEpithelial cell typesObstructive pulmonary diseaseNKCC1 transporterTh2-drivenCFTR channelsAirway epithelia311 Prime editing enables efficient, precise correction of CFTR F508del and functional rescue in human airway epithelial cells
Sousa A, Hemez C, Lei L, Traore S, Kulhankova K, Newby G, Doman J, Oye K, Pandey S, Karp P, McCray P, Liu D. 311 Prime editing enables efficient, precise correction of CFTR F508del and functional rescue in human airway epithelial cells. Journal Of Cystic Fibrosis 2024, 23: s165-s166. DOI: 10.1016/s1569-1993(24)01151-2.Peer-Reviewed Original ResearchSystematic optimization of prime editing for the efficient functional correction of CFTR F508del in human airway epithelial cells
Sousa A, Hemez C, Lei L, Traore S, Kulhankova K, Newby G, Doman J, Oye K, Pandey S, Karp P, McCray P, Liu D. Systematic optimization of prime editing for the efficient functional correction of CFTR F508del in human airway epithelial cells. Nature Biomedical Engineering 2024, 9: 7-21. PMID: 38987629, PMCID: PMC11754097, DOI: 10.1038/s41551-024-01233-3.Peer-Reviewed Original ResearchConceptsAirway epithelial cellsCystic fibrosisEpithelial cellsImmortalized bronchial epithelial cellsHuman airway epithelial cellsPrimary airway cellsCFTR ion channelBronchial epithelial cellsHuman cystic fibrosisT cellsAirway cellsOne-time treatmentThree-nucleotide deletionFunctional restorationPredominant causeHEK293T cellsIon channelsF508delCFTRMismatch repair proteinsWild-type levelsDouble-stranded DNA breaksCellsRepair proteinsDNA breaksDynamic measurement of airway surface liquid volume with an ex vivo trachea-chip
Scott M, Lei L, Bierstedt K, McCray P, Xie Y. Dynamic measurement of airway surface liquid volume with an ex vivo trachea-chip. Lab On A Chip 2024, 24: 3093-3100. PMID: 38779981, PMCID: PMC11165946, DOI: 10.1039/d4lc00134f.Peer-Reviewed Original Research
2023
CFTR-rich ionocytes mediate chloride absorption across airway epithelia
Lei L, Traore S, Ibarra G, Karp P, Rehman T, Meyerholz D, Zabner J, Stoltz D, Sinn P, Welsh M, McCray P, Thornell I. CFTR-rich ionocytes mediate chloride absorption across airway epithelia. Journal Of Clinical Investigation 2023, 133: e171268. PMID: 37581935, PMCID: PMC10575720, DOI: 10.1172/jci171268.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorAirway surface liquidAirway epitheliaLevels of cystic fibrosis transmembrane conductance regulatorApical cystic fibrosis transmembrane conductance regulatorAirway surfaceVolume of airway surface liquidLiquid secretionSurface liquidApical Na+ channelsTransmembrane conductance regulatorAirway epithelial cellsTransepithelial voltageNa+ channelsConductance regulatorBasolateral membraneCystic fibrosisInhaled pathogensPulmonary ionocyteEpithelial cellsAirwaySecretory cellsChloride absorptionCl- flowEpithelia
2022
Virus-like particles containing a prefusion-stabilized F protein induce a balanced immune response and confer protection against respiratory syncytial virus infection in mice
Luo J, Qin H, Lei L, Lou W, Li R, Pan Z. Virus-like particles containing a prefusion-stabilized F protein induce a balanced immune response and confer protection against respiratory syncytial virus infection in mice. Frontiers In Immunology 2022, 13: 1054005. PMID: 36578490, PMCID: PMC9792133, DOI: 10.3389/fimmu.2022.1054005.Peer-Reviewed Original ResearchConceptsRespiratory syncytial virusRespiratory syncytial virus vaccine candidateRespiratory syncytial virus infectionCD25+Post-FIL-5 cytokine productionIL-17A-producing cellsImmune responseCD4+ T cellsRespiratory syncytial virus fusionVaccine candidatesEvidence of immunopathologyLicensed RSV vaccineVirus-like particlesDownregulation of IL-4RSV-neutralizing antibodiesPre-FLungs of miceRSV vaccineT cellsImmunological propertiesIFN-gSyncytial virusIL-5IL-2
2021
Chimeric enterovirus 71 virus-like particle displaying conserved coxsackievirus A16 epitopes elicits potent immune responses and protects mice against lethal EV71 and CA16 infection
Luo J, Huo C, Qin H, Hu J, Lei L, Pan Z. Chimeric enterovirus 71 virus-like particle displaying conserved coxsackievirus A16 epitopes elicits potent immune responses and protects mice against lethal EV71 and CA16 infection. Vaccine 2021, 39: 4135-4143. PMID: 34116877, DOI: 10.1016/j.vaccine.2021.05.093.Peer-Reviewed Original ResearchConceptsEV71-VLPsNeutralizing antibodiesHand-foot-and-mouth diseaseNeonatal miceCA16 infectionImmune responseEV71-specific IgGVirus-like particlesEV71 virus-like particlesMultivalent vaccine strategySplenocytes of immunized miceSera of immunized miceLethal EV71 challengeCellular immune responsesDisease of infantsNeutralizing antibody productionInfectious diseasesTh2 typeCytokine secretionEV71 challengeNeutralizing epitopesVaccination strategiesChimeric VLPsEnterovirus A speciesMiceConstruction and immunological evaluation of hepatitis B virus core virus-like particles containing multiple antigenic peptides of respiratory syncytial virus
Lei L, Qin H, Luo J, Tan Y, Yang J, Pan Z. Construction and immunological evaluation of hepatitis B virus core virus-like particles containing multiple antigenic peptides of respiratory syncytial virus. Virus Research 2021, 298: 198410. PMID: 33819519, DOI: 10.1016/j.virusres.2021.198410.Peer-Reviewed Original Research
2020
Y-Box-Binding Protein 3 (YBX3) Restricts Influenza A Virus by Interacting with Viral Ribonucleoprotein Complex and Imparing its Function.
Qin Z, Qu X, Lei L, Xu L, Pan Z. Y-Box-Binding Protein 3 (YBX3) Restricts Influenza A Virus by Interacting with Viral Ribonucleoprotein Complex and Imparing its Function. Journal Of General Virology 2020, 101: 385-398. PMID: 32553055, DOI: 10.1099/jgv.0.001390.Peer-Reviewed Original ResearchMeSH KeywordsA549 CellsAnimalsCCAAT-Enhancer-Binding ProteinsCell NucleusCytoplasmDNA-Directed RNA PolymerasesDogsHeat-Shock ProteinsHEK293 CellsHost Microbial InteractionsHumansInfluenza A virusMadin Darby Canine Kidney CellsMass SpectrometryMiceNuclear ProteinsProtein BindingRibonucleoproteinsRNA, Small InterferingRNA, ViralTranscription, GeneticUp-RegulationViral Core ProteinsVirus ReplicationConceptsInfluenza A virus replicationInfluenza A virusStrep-tagRNAi knockdownNuclear accumulationViral primary transcriptionViral ribonucleoprotein complexesHost factorsVirus replicationMouse-adapted influenza A virusVirus growth rateA virusHEK 293T cellsPolymerase subunitsProtein 3PB2 subunitVRNA synthesisRibonucleoprotein complexViral protein expressionPrimary transcriptRNA transcriptsAffinity purificationInfect human cellsVRNPNegative regulator
Get In Touch
Contacts
Email