Anis Barmada, MPhil
About
Research
Publications
2025
Early-life human CD8+ T cells exhibit rapid, short-lived effector responses and a unique transcription factor landscape
Brodsky N, Chakder M, Kumar D, Barmada A, Wang J, Gu W, Olaloye O, Ramaswamy A, Wats A, Konnikova L, Lucas C. Early-life human CD8+ T cells exhibit rapid, short-lived effector responses and a unique transcription factor landscape. Proceedings Of The National Academy Of Sciences Of The United States Of America 2025, 122: e2421106122. PMID: 40720649, PMCID: PMC12337316, DOI: 10.1073/pnas.2421106122.Peer-Reviewed Original ResearchConceptsCD8+ T cellsHuman CD8+ T cellsKiller cell lectin-like receptor G1Neonatal CD8+ T cellsT cellsTranscription factor landscapeIL-2Human CD8+ T cellsCD8+ T cellsDNAX accessory molecule-1Tumor necrosis factor-alphaT cell receptor activationInnate-like characteristicsNecrosis factor-alphaResponse to viral infectionTissue immunopathologyNeonatal humansEffector programIL-7Interleukin-2Factor-alphaThymocyte selectionViral illnessEffector responsesMolecule-1
2024
PI3Kγ in B cells promotes antibody responses and generation of antibody-secreting cells
Lanahan S, Yang L, Jones K, Qi Z, Cabrera E, Cominsky L, Ramaswamy A, Barmada A, Gabernet G, Uthaya Kumar D, Xu L, Shan P, Wymann M, Kleinstein S, Rao V, Mustillo P, Romberg N, Abraham R, Lucas C. PI3Kγ in B cells promotes antibody responses and generation of antibody-secreting cells. Nature Immunology 2024, 25: 1422-1431. PMID: 38961274, PMCID: PMC12449845, DOI: 10.1038/s41590-024-01890-1.Peer-Reviewed Original ResearchAntibody-secreting cellsMemory B cellsB cellsKinase activity-dependent mannerDifferentiation of antibody-secreting cellsAntibody responseGeneration of antibody-secreting cellsInborn errors of immunityActivity-dependent mannerMouse immunization modelFate choiceT cell-dependent antigensToll-like receptor activationTranscriptional programsB cell biologyPhosphoinositide 3-kinase-gammaErrors of immunityNaive B cellsTransduce signalsActivated B cellsB lineage cellsRobust antibody responsesHumoral defectsHumoral immunityReceptor activation
2023
Single‐cell multi‐omics analysis of COVID‐19 patients with pre‐existing autoimmune diseases shows aberrant immune responses to infection
Barmada A, Handfield L, Godoy‐Tena G, de la Calle‐Fabregat C, Ciudad L, Arutyunyan A, Andrés‐León E, Hoo R, Porter T, Oszlanczi A, Richardson L, Calero‐Nieto F, Wilson N, Marchese D, Sancho‐Serra C, Carrillo J, Presas‐Rodríguez S, Ramo‐Tello C, Ruiz‐Sanmartin A, Ferrer R, Ruiz‐Rodriguez J, Martínez‐Gallo M, Munera‐Campos M, Carrascosa J, Göttgens B, Heyn H, Prigmore E, Casafont‐Solé I, Solanich X, Sánchez‐Cerrillo I, González‐Álvaro I, Raimondo M, Ramming A, Martin J, Martínez‐Cáceres E, Ballestar E, Vento‐Tormo1 R, Rodríguez‐Ubreva J. Single‐cell multi‐omics analysis of COVID‐19 patients with pre‐existing autoimmune diseases shows aberrant immune responses to infection. European Journal Of Immunology 2023, 54: e2350633. PMID: 37799110, DOI: 10.1002/eji.202350633.Peer-Reviewed Original ResearchSARS-CoV-2 infectionAutoimmune disease groupImmune responseAutoimmune diseasesDisease groupPre-existing autoimmune diseasePeripheral blood mononuclear cellsPre-existing autoimmune conditionsPre-existing autoimmunitySevere COVID-19Aberrant immune responseGroup of patientsInflammatory T cellsBlood mononuclear cellsCOVID-19 patientsMajor autoimmune diseasesCOVID-19SARS-CoV-2Autoimmune conditionsMononuclear cellsT cellsMonocyte responsePatientsSingle-cell multi-omics analysisInfectionCytokinopathy with aberrant cytotoxic lymphocytes and profibrotic myeloid response in SARS-CoV-2 mRNA vaccine–associated myocarditis
Barmada A, Klein J, Ramaswamy A, Brodsky N, Jaycox J, Sheikha H, Jones K, Habet V, Campbell M, Sumida T, Kontorovich A, Bogunovic D, Oliveira C, Steele J, Hall E, Pena-Hernandez M, Monteiro V, Lucas C, Ring A, Omer S, Iwasaki A, Yildirim I, Lucas C. Cytokinopathy with aberrant cytotoxic lymphocytes and profibrotic myeloid response in SARS-CoV-2 mRNA vaccine–associated myocarditis. Science Immunology 2023, 8: eadh3455-eadh3455. PMID: 37146127, PMCID: PMC10468758, DOI: 10.1126/sciimmunol.adh3455.Peer-Reviewed Original ResearchConceptsMRNA vaccinesSARS-CoV-2 mRNA vaccinesSARS-CoV-2 mRNA vaccinationC-reactive protein levelsB-type natriuretic peptidePeripheral blood mononuclear cellsCardiac tissue inflammationDeep immune profilingSerum soluble CD163Vaccine-associated myocarditisCohort of patientsBlood mononuclear cellsCytotoxic T cellsLate gadolinium enhancementHypersensitivity myocarditisElevated troponinMRNA vaccinationImaging abnormalitiesNK cellsImmune profilingKiller cellsMyeloid responseNatriuretic peptideHumoral mechanismsInflammatory cytokines
2022
Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines
Godoy-Tena G, Barmada A, Morante-Palacios O, de la Calle-Fabregat C, Martins-Ferreira R, Ferreté-Bonastre A, Ciudad L, Ruiz-Sanmartín A, Martínez-Gallo M, Ferrer R, Ruiz-Rodriguez J, Rodríguez-Ubreva J, Vento-Tormo R, Ballestar E. Epigenetic and transcriptomic reprogramming in monocytes of severe COVID-19 patients reflects alterations in myeloid differentiation and the influence of inflammatory cytokines. Genome Medicine 2022, 14: 134. PMID: 36443794, PMCID: PMC9706884, DOI: 10.1186/s13073-022-01137-4.Peer-Reviewed Original ResearchConceptsDNA methylation alterationsSevere COVID-19 patientsInterferon-related genesCOVID-19 patientsCell typesMethylation alterationsMyeloid differentiationSingle-cell transcriptomesSingle-cell transcriptomicsDNA methylation changesGene expression changesPeripheral blood monocytesImmune cell typesMethylationEPIC BeadChip arraySpecific DNA methylation alterationsTranscriptional reprogrammingDNA methylomeTranscriptomic reprogrammingDNA methylationInflammatory cytokinesMethylation changesEpigenetic alterationsBlood monocytesExpression changesBeadChip array
2021
Maximizing insights from monogenic immune disorders
Barmada A, Ramaswamy A, Lucas CL. Maximizing insights from monogenic immune disorders. Current Opinion In Immunology 2021, 73: 50-57. PMID: 34695727, PMCID: PMC8915947, DOI: 10.1016/j.coi.2021.09.008.Peer-Reviewed Original ResearchConceptsDisease gene discoveryGene discoverySingle geneIntegrative omicsMonogenic immune disordersHuman diseasesUnprecedented insightsMouse modelingFundamental immune functionsImmune disordersMechanistic studiesGenomeGenomicsImmune functionOmicsDisease discoveryDiscoveryGenesInsightsDisordersDisease
2020
Quantifying Sample Collection and Processing Impacts on Fiber-Based Tear Fluid Chemical Analysis
Barmada A, Shippy S. Quantifying Sample Collection and Processing Impacts on Fiber-Based Tear Fluid Chemical Analysis. Translational Vision Science & Technology 2020, 9: 23-23. PMID: 33024616, PMCID: PMC7521181, DOI: 10.1167/tvst.9.10.23.Peer-Reviewed Original ResearchTear analysis as the next routine body fluid test
Barmada A, Shippy S. Tear analysis as the next routine body fluid test. Eye 2020, 34: 1731-1733. PMID: 32376979, PMCID: PMC7608258, DOI: 10.1038/s41433-020-0930-0.Peer-Reviewed Original Research
2018
Thread-based assay for quantitative small molecule analysis of mice tear fluid by capillary electrophoresis
Barmada A, Shippy S. Thread-based assay for quantitative small molecule analysis of mice tear fluid by capillary electrophoresis. Analytical And Bioanalytical Chemistry 2018, 411: 329-338. PMID: 30460389, DOI: 10.1007/s00216-018-1488-6.Peer-Reviewed Original ResearchConceptsSmall molecule analysisSmall moleculesMolecule analysisMouse tearsChemical compositionCapillary electrophoresis separationMinimal sample handlingHigh separation resolutionSmall volume analysisTear fluidLight-emitting diode-induced fluorescence detectionSeparation resolutionHuman tearsCapillary electrophoresisElectrophoresis separationDiode-induced fluorescence detectionOcular surface diseaseCD-1 malesMicromolar rangePhenol red threadFluorescence detectionSample handlingMoleculesLevels of arginineNon-invasive assay
Academic Achievements & Community Involvement
News
News
- December 04, 2024
4th Year MD-PhD Student Anis Barmada Named to the 2025 Forbes 30 Under 30 List
- May 05, 2023Source: Yale News
Yale Study Reveals Insights Into Post-Vaccine Heart Inflammation Cases
- May 05, 2023Source: Stat News
What explains rare heart condition among young men after Covid vaccines? A new study offers clues