In-Hyun Park, PhD
Associate Professor of Genetics and Associate Professor in the Child Study Center; Yale Stem Cell Center
Biography
Research & Publications
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Research Summary
Overexpression of four factors (Oct4, Sox2, Klf4, Myc, or
Oct4, Sox2, Nanog, Lin28) reprogram somatic cells to become induced pluripotent
stem (iPS) cells. Reprogramming accompanies genetic and epigenetic changes, and
its molecular mechanism is still unknown. We recently showed that in iPS cells
the global DNA methylation status is close to that of human embryonic stem
(hES) cells, suggesting the epigenetic resetting during reprogramming.
Furthermore, we have showed the possible dissection of stages in reprogramming
through live cell imaging analysis.
We will investigate the molecular mechanism of genetic and epigenetic change during reprogramming. iPS cells show similar characteristics as hES cells, such as self-renewal and pluripotency, and provide an incredible resource for cell-based therapy, in vitro disease model and screening drugs.
We will investigate the molecular mechanism of genetic and epigenetic change during reprogramming. iPS cells show similar characteristics as hES cells, such as self-renewal and pluripotency, and provide an incredible resource for cell-based therapy, in vitro disease model and screening drugs.
Extensive Research Description
- Investigation of genetic and epigenetic regulation of reprogramming
- in vitro model of human neurodevelopmental disease
Research Interests
Cells; Central Nervous System Diseases; Nervous System; Stem Cells; Pluripotent Stem Cells; Embryonic Stem Cells; Induced Pluripotent Stem Cells; Neural Stem Cells; Psychiatry and Psychology
Selected Publications
- Transcriptome Signature and Regulation in Human Somatic Cell Reprogramming.Tanaka Y, Hysolli E, Su J, Xiang Y, Kim KY, Zhong M, Li Y, Heydari K, Euskirchen G, Snyder MP, Pan X, Weissman SM, Park IH. Transcriptome Signature and Regulation in Human Somatic Cell Reprogramming. Stem Cell Reports 2015, 4:1125-39.
- X Chromosome of female cells shows dynamic changes in status during human somatic cell reprogramming.Kim KY, Hysolli E, Tanaka Y, Wang B, Jung YW, Pan X, Weissman SM, Park IH. X Chromosome of female cells shows dynamic changes in status during human somatic cell reprogramming. Stem Cell Reports 2014, 2:896-909.
- Transcriptional regulation in pluripotent stem cells by methyl CpG-binding protein 2 (MeCP2).Tanaka Y, Kim KY, Zhong M, Pan X, Weissman SM, Park IH. Transcriptional regulation in pluripotent stem cells by methyl CpG-binding protein 2 (MeCP2). Human Molecular Genetics 2014, 23:1045-55.
- Pluripotent stem cell models of Shwachman-Diamond syndrome reveal a common mechanism for pancreatic and hematopoietic dysfunction.Tulpule A, Kelley JM, Lensch MW, McPherson J, Park IH, Hartung O, Nakamura T, Schlaeger TM, Shimamura A, Daley GQ. Pluripotent stem cell models of Shwachman-Diamond syndrome reveal a common mechanism for pancreatic and hematopoietic dysfunction. Cell Stem Cell 2013, 12:727-36.
- Cellular reprogramming: a novel tool for investigating autism spectrum disorders.Kim KY, Jung YW, Sullivan GJ, Chung L, Park IH. Cellular reprogramming: a novel tool for investigating autism spectrum disorders. Trends In Molecular Medicine 2012, 18:463-71.
- Neuronal maturation defect in induced pluripotent stem cells from patients with Rett syndrome.Kim KY, Hysolli E, Park IH. Neuronal maturation defect in induced pluripotent stem cells from patients with Rett syndrome. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108:14169-74.
- Telomere elongation in induced pluripotent stem cells from dyskeratosis congenita patients.Agarwal S, Loh YH, McLoughlin EM, Huang J, Park IH, Miller JD, Huo H, Okuka M, Dos Reis RM, Loewer S, Ng HH, Keefe DL, Goldman FD, Klingelhutz AJ, Liu L, Daley GQ. Telomere elongation in induced pluripotent stem cells from dyskeratosis congenita patients. Nature 2010, 464:292-6.
- Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells.Chan EM, Ratanasirintrawoot S, Park IH, Manos PD, Loh YH, Huo H, Miller JD, Hartung O, Rho J, Ince TA, Daley GQ, Schlaeger TM. Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells. Nature Biotechnology 2009, 27:1033-7.
- Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts.Doi A, Park IH, Wen B, Murakami P, Aryee MJ, Irizarry R, Herb B, Ladd-Acosta C, Rho J, Loewer S, Miller J, Schlaeger T, Daley GQ, Feinberg AP. Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts. Nature Genetics 2009, 41:1350-3.
- Disease-specific induced pluripotent stem cells.Park IH, Arora N, Huo H, Maherali N, Ahfeldt T, Shimamura A, Lensch MW, Cowan C, Hochedlinger K, Daley GQ. Disease-specific induced pluripotent stem cells. Cell 2008, 134:877-86.