2012
Reprogramming human somatic cells into induced pluripotent stem cells (iPSCs) using retroviral vector with GFP.
Kim KY, Hysolli E, Park IH. Reprogramming human somatic cells into induced pluripotent stem cells (iPSCs) using retroviral vector with GFP. Journal Of Visualized Experiments 2012 PMID: 22491226, PMCID: PMC3466658, DOI: 10.3791/3804.Peer-Reviewed Original ResearchConceptsHuman embryonic stem cellsInduced pluripotent stem cellsHuman somatic cellsHuman induced pluripotent stem cellsPluripotent stem cellsSomatic cellsIPSC coloniesStem cellsESC culture conditionsEmbryonic stem cellsPluripotency genesTranscription factorsRetroviral transgenesEctopic expressionGFP fluorescenceRetroviral vectorsHuman fibroblast cellsFibroblast cellsGFPCulture conditionsCellsAutologous cellsCellular sourceColoniesSurface markersHuman induced pluripotent stem cells and neurodegenerative disease
Jung YW, Hysolli E, Kim KY, Tanaka Y, Park IH. Human induced pluripotent stem cells and neurodegenerative disease. Current Opinion In Neurology 2012, 25: 125-130. PMID: 22357218, PMCID: PMC3786112, DOI: 10.1097/wco.0b013e3283518226.Peer-Reviewed Original ResearchConceptsNeurodegenerative diseasesReplacement therapyCell replacement therapyParkinson's diseasePositive therapeutic effectDisease modelsParkinson's disease modelAmyotrophic lateral sclerosisNeuron-specific transcription factorSpinal muscular atrophyStem cellsDrug screeningDopaminergic neuronsIntermediate cell typeTherapeutic effectEffective treatmentLateral sclerosisDisease-specific iPSCsMuscular atrophyAlzheimer's diseaseNeurodegenerative disordersCellular therapyDiseaseTherapyHuman induced pluripotent stem cells
2009
Hematopoietic Development From Human Induced Pluripotent Stem Cells.
Grauer M, Konantz M, Niebuhr N, Kanz L, Park I, Daley G, Lengerke C. Hematopoietic Development From Human Induced Pluripotent Stem Cells. Blood 2009, 114: 2530. DOI: 10.1182/blood.v114.22.2530.2530.Peer-Reviewed Original ResearchMouse embryonic stem cellsEmbryonic stem cellsInduced pluripotent stem cellsPluripotent stem cellsHuman embryonic stem cellsHematopoietic stem cellsHuman induced pluripotent stem cellsHuman iPS cellsIPS cellsCdx genesHematopoietic developmentBlood lineagesStem cellsBlood formationEmbryonic blood formationGenetic modificationHuman developmental hematopoiesisDifferentiated somatic cellsHuman pluripotent stem cell linesStem cell linesIrradiated adult micePluripotent stem cell lineDevelopmental hematopoiesisHematopoietic genesHox genesDifferential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts
Doi A, Park IH, Wen B, Murakami P, Aryee MJ, Irizarry R, Herb B, Ladd-Acosta C, Rho J, Loewer S, Miller J, Schlaeger T, Daley GQ, Feinberg AP. Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts. Nature Genetics 2009, 41: 1350-1353. PMID: 19881528, PMCID: PMC2958040, DOI: 10.1038/ng.471.Peer-Reviewed Original ResearchCardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells
Zwi L, Caspi O, Arbel G, Huber I, Gepstein A, Park IH, Gepstein L. Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells. Circulation 2009, 120: 1513-1523. PMID: 19786631, DOI: 10.1161/circulationaha.109.868885.Peer-Reviewed Original ResearchConceptsTranscription factorsHiPS cellsCardiomyocyte differentiationCardiac-specific transcription factorsInduced pluripotent stem cellsGene expression studiesHuman induced pluripotent stem cellsStem cell linesPluripotent stem cellsCardiomyocyte differentiation potentialPluripotent stem cell lineStructural geneContracting embryoid bodiesCardiomyocyte differentiation processTranslational cardiovascular researchEmbryoid bodiesExpression studiesDifferentiation systemDifferentiation processCardiovascular regenerative medicineSarcomeric proteinsDifferentiation potentialIon channelsAdult fibroblastsFunctional propertiesHematopoietic Development from Human Induced Pluripotent Stem Cells
Lengerke C, Grauer M, Niebuhr NI, Riedt T, Kanz L, Park I, Daley GQ. Hematopoietic Development from Human Induced Pluripotent Stem Cells. Annals Of The New York Academy Of Sciences 2009, 1176: 219-227. PMID: 19796250, PMCID: PMC2849804, DOI: 10.1111/j.1749-6632.2009.04606.x.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsMouse embryonic stem cellsPluripotent stem cellsHematopoietic developmentInduced pluripotent stem cellsHuman induced pluripotent stem cellsHematopoietic stem cellsStem cellsDifferentiated somatic cellsHuman embryonic stem cellsHuman hematopoietic developmentStem cell linesGene expression dataIrradiated adult miceHox genesPluripotent stateBlood lineagesSomatic cellsEctopic expressionCombinatorial overexpressionExpression dataBlood formationIPS cellsColony activityMultilineage reconstitution