2011
FGF Signaling Expands Embryonic Cortical Surface Area by Regulating Notch-Dependent Neurogenesis
Rash BG, Lim HD, Breunig JJ, Vaccarino FM. FGF Signaling Expands Embryonic Cortical Surface Area by Regulating Notch-Dependent Neurogenesis. Journal Of Neuroscience 2011, 31: 15604-15617. PMID: 22031906, PMCID: PMC3235689, DOI: 10.1523/jneurosci.4439-11.2011.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnalysis of VarianceAnimalsBrainBromodeoxyuridineCaspase 3Cell CountCell DifferentiationCells, CulturedCerebral CortexDNA-Binding ProteinsElectroporationEmbryo, MammalianEye ProteinsFatty Acid-Binding Protein 7Fatty Acid-Binding ProteinsFibroblast Growth FactorsGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsHomeodomain ProteinsKi-67 AntigenMiceMice, TransgenicMutationNerve Tissue ProteinsNeurogenesisNeuronsPaired Box Transcription FactorsPAX6 Transcription FactorReceptors, Fibroblast Growth FactorReceptors, NotchRepressor ProteinsSignal TransductionStem CellsT-Box Domain ProteinsTranscription FactorsConceptsCortical neurogenesisCortical surface area expansionCortical surface expansionCortical surface areaGrowth factor receptorEmbryonic day 12.5Fibroblast growth factor receptorFGFR mutantsNormal miceCortical layer structureCortical developmentNeurogenic stagesDominant negative FGFRLoss of functionRadial progenitorsNeurogenesisNotch pathway genesSevere deficitsFactor receptorDay 12.5Notch pathwayMiceSimultaneous activationGreater proportionFGFR activity
2010
Pyramidal Neurons Are Generated from Oligodendroglial Progenitor Cells in Adult Piriform Cortex
Guo F, Maeda Y, Ma J, Xu J, Horiuchi M, Miers L, Vaccarino F, Pleasure D. Pyramidal Neurons Are Generated from Oligodendroglial Progenitor Cells in Adult Piriform Cortex. Journal Of Neuroscience 2010, 30: 12036-12049. PMID: 20826667, PMCID: PMC2940828, DOI: 10.1523/jneurosci.1360-10.2010.Peer-Reviewed Original ResearchMeSH KeywordsAdult Stem CellsAnimalsAntigensAntineoplastic Agents, HormonalBromodeoxyuridineCell CountCell DifferentiationCerebral CortexDoublecortin Domain ProteinsDrug Administration ScheduleEye ProteinsGene Expression RegulationGreen Fluorescent ProteinsHomeodomain ProteinsMiceMice, Inbred C57BLMice, TransgenicMicrotubule-Associated ProteinsMyelin Proteolipid ProteinNerve Tissue ProteinsNeuronsNeuropeptidesOligodendrogliaPaired Box Transcription FactorsPAX6 Transcription FactorProteoglycansPyramidal CellsReceptor, Platelet-Derived Growth Factor alphaReceptors, N-Methyl-D-AspartateRepressor ProteinsSOXB1 Transcription FactorsTamoxifenTime FactorsConceptsOligodendroglial progenitor cellsPyramidal glutamatergic neuronsPiriform cortexAdult piriform cortexGlutamatergic neuronsCortical glutamatergic neuronsProgenitor cellsNeural stem cell markersCortical neuronal networksStem cell markersTranscription factor characteristicImmature neuronsCerebral cortexPyramidal neuronsCell markersCortexNeuronsCre-loxP recombination systemNeuronal networksLines of evidenceMarkersLow levelsCellsPrevious studiesDoublecortin
2005
Otx2 Regulates Subtype Specification and Neurogenesis in the Midbrain
Vernay B, Koch M, Vaccarino F, Briscoe J, Simeone A, Kageyama R, Ang SL. Otx2 Regulates Subtype Specification and Neurogenesis in the Midbrain. Journal Of Neuroscience 2005, 25: 4856-4867. PMID: 15888661, PMCID: PMC6724764, DOI: 10.1523/jneurosci.5158-04.2005.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnimalsAnimals, NewbornBasic Helix-Loop-Helix Transcription FactorsBody PatterningBromodeoxyuridineCell CountCell DifferentiationDopamineEmbryo, MammalianEmbryonic InductionFibroblast Growth Factor 8Gene Expression Regulation, DevelopmentalHomeobox Protein Nkx-2.2Homeodomain ProteinsImmunohistochemistryIn Situ HybridizationIn Situ Nick-End LabelingIntermediate Filament ProteinsIntracellular Signaling Peptides and ProteinsKruppel-Like Transcription FactorsMembrane ProteinsMesencephalonMiceMice, TransgenicNerve Tissue ProteinsNestinNeuronsOrganizers, EmbryonicOtx Transcription FactorsPatched ReceptorsReceptors, Cell SurfaceSerotoninTranscription FactorsWnt1 ProteinZebrafish ProteinsZinc Finger Protein GLI1ConceptsMid-hindbrain organizerFunction of Otx2Neuronal subtype identityTranscription factor Otx2Rostral brain developmentEmbryonic day 10.5Cre/loxP systemRole of Otx2Neuronal progenitor cellsMutant embryosProgenitor identityConditional mutantsE10.5 onwardOtx2 activityAnterior hindbrainEctopic expressionCerebellar-like structuresSubtype specificationSubtype identityTransgenic animalsNovel roleLater roleNovel mouse modelOtx2Otx2 expression
2000
The subcellular localization of OTX2 is cell-type specific and developmentally regulated in the mouse retina
Baas D, Bumsted KM, Martinez JA, Vaccarino FM, Wikler KC, Barnstable CJ. The subcellular localization of OTX2 is cell-type specific and developmentally regulated in the mouse retina. Brain Research 2000, 78: 26-37. PMID: 10891582, DOI: 10.1016/s0169-328x(00)00060-7.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsAntibodiesBlotting, WesternCell NucleusCytoplasmGene Expression Regulation, DevelopmentalHomeodomain ProteinsHumansMiceMice, Inbred StrainsNerve Tissue ProteinsOtx Transcription FactorsPC12 CellsPigment Epithelium of EyeRabbitsRatsRetinal Ganglion CellsRetinal Rod Photoreceptor CellsTeratocarcinomaTrans-ActivatorsTransfectionTumor Cells, CulturedConceptsSubcellular localizationTranscription factorsHomeodomain-containing proteinCell fate determinationHomeodomain transcription factorCytoplasm of rodsFate determinationCell fateOtx2 proteinSubcellular distributionOtx2Retinal pigment epithelial cellsCell typesRod photoreceptorsPigment epithelial cellsRetinal developmentCytoplasmCell linesAdult eyesEpithelial cellsCentral nervous systemImmature rodsProteinCellsDifferential distribution
1999
Progressive impairment of developing neuroendocrine cell lineages in the hypothalamus of mice lacking the Orthopedia gene
Acampora D, Postiglione M, Avantaggiato V, Di Bonito M, Vaccarino F, Michaud J, Simeone A. Progressive impairment of developing neuroendocrine cell lineages in the hypothalamus of mice lacking the Orthopedia gene. Genes & Development 1999, 13: 2787-2800. PMID: 10557207, PMCID: PMC317121, DOI: 10.1101/gad.13.21.2787.Peer-Reviewed Original ResearchConceptsCorticotropin-releasing hormoneAnterior periventricularProgressive impairmentArginine vasopressinHypothalamus of miceTerminal differentiationNeuroendocrine cell lineagesCell lineagesNeuronal cell lineagesMagnocellular neuronsNeuroendocrine hypothalamusAbnormal cell migrationParaventricularCell proliferationHypothalamusCell migrationMiceBRN2 expressionNeuronsImpairmentOxytocinPeriventricularDifferentiationIdentification, Chromosomal Assignment, and Expression Analysis of the Human Homeodomain-Containing Gene Orthopedia (OTP)
Lin X, State M, Vaccarino F, Greally J, Hass M, Leckman J. Identification, Chromosomal Assignment, and Expression Analysis of the Human Homeodomain-Containing Gene Orthopedia (OTP). Genomics 1999, 60: 96-104. PMID: 10458915, DOI: 10.1006/geno.1999.5882.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBrainChromosome BandingChromosome MappingChromosomes, Human, Pair 5DNA, ComplementaryDrosophila ProteinsExonsGene ExpressionGene Expression Regulation, DevelopmentalGenesHomeodomain ProteinsHumansImmunohistochemistryIn Situ Hybridization, FluorescenceIntronsMolecular Sequence DataNerve Tissue ProteinsRatsRats, Sprague-DawleySequence AlignmentSequence Analysis, DNASequence Homology, Amino AcidConceptsHuman cDNAHuman homologueRadiation hybrid panel mappingDeduced amino acid sequenceHelix transcription factorOpen reading frameAmino acid sequenceHuman HomeodomainChromosomal assignmentHomeodomain genesCell fateGenomic libraryTranscription factorsHuman chromosomesReading frameGene productsAcid sequenceHD geneExpression analysisC-terminusYAC clonesNovel polyclonal antibodyOrthopediaCell migration patternsHuman fetal brain tissue
1997
Dlx-2 homeobox gene controls neuronal differentiation in primary cultures of developing basal ganglia
Ding M, Robel L, James A, Eisenstat D, Leckman J, Rubenstein J, Vaccarino F. Dlx-2 homeobox gene controls neuronal differentiation in primary cultures of developing basal ganglia. Journal Of Molecular Neuroscience 1997, 8: 93-113. PMID: 9188040, DOI: 10.1007/bf02736776.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibody SpecificityAntisense Elements (Genetics)Basal GangliaCell DifferentiationCell DivisionCells, CulturedCytoskeletal ProteinsDNA-Binding ProteinsFemaleGene Expression Regulation, DevelopmentalGenes, HomeoboxHomeodomain ProteinsIn Situ HybridizationMicrotubule-Associated ProteinsNeuritesNeuronsPhenotypePregnancyRatsRhombencephalonRNA-Binding ProteinsTelencephalonTranscription FactorsConceptsGene productsNeuronal differentiationMicrotubule-associated protein MAP1BHomeodomain-containing genesDlx-2Homeobox genesNeuronal polarityCellular phenotypesNeuronal lineageProtein MAP1BPrimary culturesCellular localizationMitotic cycleGlial fibrillary acidic proteinGenesProteinCell proliferationDendrite outgrowthExpression of MAP2MAP2-positive dendritesNeuronal dendritesNeurofilament subunitsExpressionMAP2 expressionMRNA
1996
Characterization and Sequence Analysis of the Human Homeobox-Containing GeneGBX2
Lin X, Swaroop A, Vaccarino F, Murtha M, Haas M, Ji X, Ruddle F, Leckman J. Characterization and Sequence Analysis of the Human Homeobox-Containing GeneGBX2. Genomics 1996, 31: 335-342. PMID: 8838315, DOI: 10.1006/geno.1996.0056.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBrainCell LineChromosome MappingChromosomes, Human, Pair 2Cloning, MolecularDNA PrimersDNA, ComplementaryFemaleGene ExpressionGenomic LibraryHomeodomain ProteinsHumansMolecular Sequence DataPregnancyRodentiaSequence AnalysisSequence Homology, Amino AcidConceptsFetal brain cDNA libraryBrain cDNA libraryCDNA clonesCDNA librarySomatic cell hybrid analysisHuman fetal brain cDNA libraryCell hybrid analysisHuman genomic cloneAmino-terminal endAmino acid residuesHuman homeoboxesGenomic clonesHomologous genesGBX2 geneHomeobox genesCpG islandsNucleotide sequenceMouse embryosUntranslated regionProline residuesNorthern analysisAcid residuesSequence analysisGenesPolymerase chain reaction