2024
Abstract 146: Phosphodiesterase 10A Mediates Arterial Calcification Through A P38/MAPK-MMP3 Signaling
Jin Y, Xie Y, Berezowitz A, Davis S, Flores A, Wang X, Guzman R, Cai Y. Abstract 146: Phosphodiesterase 10A Mediates Arterial Calcification Through A P38/MAPK-MMP3 Signaling. Arteriosclerosis Thrombosis And Vascular Biology 2024, 44: a146-a146. DOI: 10.1161/atvb.44.suppl_1.146.Peer-Reviewed Original ResearchVascular smooth muscle cellsChronic kidney diseasePeripheral arterial diseaseArtery calcificationInhibition of PDE10AMedial arterial calcificationVascular calcificationAssociated with increased cardiovascular morbidityCalcifying vascular smooth muscle cellsMatrix metallopeptidaseSmooth muscle cellsVSMCs in vitroCyclic nucleotides cAMPArteries in vivoMMP-3 upregulationCardiovascular morbidityCalcification in vivoArtery diseaseVon Kossa stainingKidney diseaseMuscle cellsRodent modelsVSMC calcificationInducible isoformCalcium assaySmooth muscle cell-specific matrix metalloproteinase 3 deletion reduces osteogenic transformation and medial artery calcification
Xie Y, Lin T, Jin Y, Berezowitz A, Wang X, Lu J, Cai Y, Guzman R. Smooth muscle cell-specific matrix metalloproteinase 3 deletion reduces osteogenic transformation and medial artery calcification. Cardiovascular Research 2024, 120: 658-670. PMID: 38454645, PMCID: PMC11074797, DOI: 10.1093/cvr/cvae035.Peer-Reviewed Original ResearchSmooth muscle cellsPeripheral arterial diseaseVascular smooth muscle cellsMedial calcificationMMP-3Osteogenic transformationRodent modelsAssociated with increased morbiditySmooth muscle cell calcificationChronic kidney diseaseEx vivo organ culture modelMMP-3 expressionOrgan culture modelMedial arterial calcificationTargeting MMP-3Matrix metalloproteinase 3MMP-3 proteinArtery calcificationArtery diseaseKidney diseaseVascular calcificationMuscle cellsTherapeutic strategiesCalcification mediumVitamin D3
2023
Perfluorooctanesulfonic Acid and Perfluorooctanoic Acid Promote Migration of Three-Dimensional Colorectal Cancer Spheroids
Zheng J, Sun B, Berardi D, Lu L, Yan H, Zheng S, Aladelokun O, Xie Y, Cai Y, Pollitt K, Khan S, Johnson C. Perfluorooctanesulfonic Acid and Perfluorooctanoic Acid Promote Migration of Three-Dimensional Colorectal Cancer Spheroids. Environmental Science And Technology 2023, 57: 21016-21028. PMID: 38064429, DOI: 10.1021/acs.est.3c04844.Peer-Reviewed Original ResearchConceptsColorectal cancerFatty acid β-oxidationCell linesSW48 cell linesSynthesis of proteinsProgression of CRCMigration phenotypeCRC cell linesEpithelial-mesenchymal transitionThree-dimensional spheroidsMetabolic pathwaysN-cadherinΒ-oxidationMechanism of actionNovel insightsE-cadherinBiological techniquesPromotes MigrationColorectal cancer spheroidsMigration assaysMetabolic profilingKRAS G12APerfluorooctanoic acidPersistent environmental contaminantsMetabolic profileSex hormones impact early maturation and immune response in the arteriovenous fistula mouse model
Satam K, Ohashi Y, Thaxton C, Gonzalez L, Setia O, Bai H, Aoyagi Y, Xie Y, Zhang W, Yatsula B, Martin K, Cai Y, Dardik A. Sex hormones impact early maturation and immune response in the arteriovenous fistula mouse model. AJP Heart And Circulatory Physiology 2023, 325: h77-h88. PMID: 37145957, PMCID: PMC10243550, DOI: 10.1152/ajpheart.00049.2023.Peer-Reviewed Original ResearchConceptsIntact female miceAVF maturationSex hormonesT cellsFemale miceArteriovenous fistulaMale miceMouse modelHigher IL-10Arteriovenous fistula creationImmune cell recruitmentSex-specific therapiesHormone receptor signalingSex differencesHuman AVF maturationAVF surgeryMale patientsClinical outcomesFemale patientsFistula maturationIL-10C57BL/6 miceInferior outcomesVenous adaptationFistula creation
2022
Role of PDE10A in vascular smooth muscle cell hyperplasia and pathological vascular remodelling.
Luo L, Cai Y, Zhang Y, Hsu CG, Korshunov VA, Long X, Knight PA, Berk BC, Yan C. Role of PDE10A in vascular smooth muscle cell hyperplasia and pathological vascular remodelling. Cardiovasc Res 2022, 118: 2703-2717. PMID: 34550322, DOI: 10.1093/cvr/cvab304.Peer-Reviewed Original Research
2021
Recovery of limb perfusion and function after hindlimb ischemia is impaired by arterial calcification
Zettervall SL, Wang X, Monk S, Lin T, Cai Y, Guzman RJ. Recovery of limb perfusion and function after hindlimb ischemia is impaired by arterial calcification. Physiological Reports 2021, 9: e15008. PMID: 34405571, PMCID: PMC8371346, DOI: 10.14814/phy2.15008.Peer-Reviewed Original ResearchConceptsPeripheral artery diseaseMedial artery calcificationFemoral artery ligationVehicle-injected ratsMedial calcificationHindlimb ischemiaArtery calcificationLimb ischemiaArtery ligationFunctional recoveryArterial calcificationLaser Doppler perfusion imagingAcute limb ischemiaChronic kidney diseaseRecovery of perfusionVascular smooth muscle cellsSmooth muscle cellsLower extremity vesselsAmputation rateArtery diseaseArterial stiffnessClinical outcomesImpaired perfusionKidney diseaseLimb perfusionCyclic nucleotide phosphodiesterase 1C contributes to abdominal aortic aneurysm.
Zhang C, Zhao H, Cai Y, Xiong J, Mohan A, Lou D, Shi H, Zhang Y, Long X, Wang J, Yan C. Cyclic nucleotide phosphodiesterase 1C contributes to abdominal aortic aneurysm. Proc Natl Acad Sci U S A 2021, 118 PMID: 34312235, DOI: 10.1073/pnas.2107898118.Peer-Reviewed Original ResearchSalt-Inducible Kinase 3 Promotes Vascular Smooth Muscle Cell Proliferation and Arterial Restenosis by Regulating AKT and PKA-CREB Signaling
Cai Y, Wang XL, Lu J, Lin X, Dong J, Guzman RJ. Salt-Inducible Kinase 3 Promotes Vascular Smooth Muscle Cell Proliferation and Arterial Restenosis by Regulating AKT and PKA-CREB Signaling. Arteriosclerosis Thrombosis And Vascular Biology 2021, 41: 2431-2451. PMID: 34196217, PMCID: PMC8411910, DOI: 10.1161/atvbaha.121.316219.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell MovementCell ProliferationCells, CulturedConstriction, PathologicCREB-Binding ProteinCyclic AMP-Dependent Protein KinasesCyclin-Dependent Kinase Inhibitor p21Cyclin-Dependent Kinase Inhibitor p27Disease Models, AnimalFemaleFemoral ArteryMaleMice, Inbred C57BLMuscle, Smooth, VascularMyocytes, Smooth MuscleNeointimaPhenylurea CompoundsProtein Kinase InhibitorsProtein Serine-Threonine KinasesProto-Oncogene Proteins c-aktPyrimidinesRats, Sprague-DawleySignal TransductionTranscription FactorsVascular System InjuriesConceptsSmooth muscle cellsArterial restenosisNeointima formationSMC proliferationSMC migrationSIK inhibitionVascular smooth muscle cell proliferationPeripheral arterial diseaseSmooth muscle cell proliferationVascular smooth muscle cellsAortic smooth muscle cellsPotential therapeutic strategyMuscle cell proliferation
2019
Medial artery calcification increases neointimal hyperplasia after balloon injury
Marshall AP, Luo W, Wang XL, Lin T, Cai Y, Guzman RJ. Medial artery calcification increases neointimal hyperplasia after balloon injury. Scientific Reports 2019, 9: 8193. PMID: 31160618, PMCID: PMC6547750, DOI: 10.1038/s41598-019-44668-4.Peer-Reviewed Original ResearchMeSH KeywordsAngioplasty, BalloonAnimalsBone Morphogenetic Protein 2CalcinosisCalcium ChlorideCarotid ArteriesCarotid Artery InjuriesCell ProliferationCoronary RestenosisDisease Models, AnimalHyperplasiaMaleMuscle, Smooth, VascularMyocytes, Smooth MuscleNeointimaProliferating Cell Nuclear AntigenRatsRats, Sprague-DawleyTunica IntimaConceptsSmooth muscle cellsNeointimal hyperplasiaBalloon injuryArterial calcificationCalcified arteriesMedial smooth muscle cellsCell nuclear antigen stainingBalloon catheter injuryMedial artery calcificationTime of injuryVascular smooth muscle cellsNeointimal cell proliferationCell proliferationCatheter injuryArtery calcificationMedial calcificationSubcutaneous injectionAntigen stainingVitamin D3Rat carotidEffect of calcificationArteryHyperplasiaAdventitial applicationInjury
2018
Inhibition of endo-lysosomal function exacerbates vascular calcification
Cai Y, Wang XL, Flores AM, Lin T, Guzman RJ. Inhibition of endo-lysosomal function exacerbates vascular calcification. Scientific Reports 2018, 8: 3377. PMID: 29467541, PMCID: PMC5821871, DOI: 10.1038/s41598-017-17540-6.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsVascular calcificationAortic rings ex vivoChronic kidney diseaseMedial artery calcificationSmooth muscle cellsLysosomal inhibitor chloroquineCardiovascular morbidityArtery calcificationPathologic responseDiabetes mellitusAortic ringsKidney diseaseMedial calcificationVitamin D3Osteogenic transformationVSMC calcificationLysosomal marker lysosomeProtective roleLysosome inhibitionInhibitor chloroquineMembrane protein 2Ex vivoMuscle cellsCalcification
2016
PDE1C deficiency antagonizes pathological cardiac remodeling and dysfunction.
Knight WE, Chen S, Zhang Y, Oikawa M, Wu M, Zhou Q, Miller CL, Cai Y, Mickelsen DM, Moravec C, Small EM, Abe J, Yan C. PDE1C deficiency antagonizes pathological cardiac remodeling and dysfunction. Proc Natl Acad Sci U S A 2016, 113: E7116-E7125. PMID: 27791092, DOI: 10.1073/pnas.1607728113.Peer-Reviewed Original ResearchDorsomorphin homologue 1, a highly selective small-molecule bone morphogenetic protein inhibitor, suppresses medial artery calcification
Lin T, Wang XL, Zettervall SL, Cai Y, Guzman RJ. Dorsomorphin homologue 1, a highly selective small-molecule bone morphogenetic protein inhibitor, suppresses medial artery calcification. Journal Of Vascular Surgery 2016, 66: 586-593. PMID: 27374065, PMCID: PMC5201454, DOI: 10.1016/j.jvs.2016.03.462.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAorta, ThoracicAortic DiseasesBone Morphogenetic Protein Receptors, Type IBone Morphogenetic ProteinsCells, CulturedHumansMuscle, Smooth, VascularMyocytes, Smooth MuscleOrgan Culture TechniquesOsteogenesisPhosphatesPyrazolesQuinolinesRats, Sprague-DawleySignal TransductionVascular CalcificationConceptsMedial artery calcificationSmooth muscle cellsArtery calcificationMedial calcificationOrgan culture modelVascular patientsVascular calcificationWestern blotCalcium accumulationHuman aortic smooth muscle cellsChronic kidney diseaseCulture modelVascular smooth muscle cellsAortic smooth muscle cellsQuantitative reverse transcription polymerase chain reactionReverse transcription-polymerase chain reactionSMC markersTranscription-polymerase chain reactionHuman smooth muscle cellsAortic medial calcificationMuscle myosin heavy chainHomologue 1Bone morphogenetic proteinKidney diseasePolymerase chain reaction
2015
Role of cAMP-phosphodiesterase 1C signaling in regulating growth factor receptor stability, vascular smooth muscle cell growth, migration, and neointimal hyperplasia.
Cai Y, Nagel DJ, Zhou Q, Cygnar KD, Zhao H, Li F, Pi X, Knight PA, Yan C. Role of cAMP-phosphodiesterase 1C signaling in regulating growth factor receptor stability, vascular smooth muscle cell growth, migration, and neointimal hyperplasia. Circ Res 2015, 116: 1120-32. PMID: 25608528, DOI: 10.1161/CIRCRESAHA.116.304408.Peer-Reviewed Original Research
2013
Cyclic nucleotide phosphodiesterase 3A1 protects the heart against ischemia-reperfusion injury.
Oikawa M, Wu M, Lim S, Knight WE, Miller CL, Cai Y, Lu Y, Blaxall BC, Takeishi Y, Abe J, Yan C. Cyclic nucleotide phosphodiesterase 3A1 protects the heart against ischemia-reperfusion injury. J Mol Cell Cardiol 2013, 64: 11-9. PMID: 23988739, DOI: 10.1016/j.yjmcc.2013.08.003.Peer-Reviewed Original ResearchVinpocetine attenuates lipid accumulation and atherosclerosis formation.
Cai Y, Li JD, Yan C. Vinpocetine attenuates lipid accumulation and atherosclerosis formation. Biochem Biophys Res Commun 2013, 434: 439-43. PMID: 23583194, DOI: 10.1016/j.bbrc.2013.03.092.Peer-Reviewed Original Research
2012
Vinpocetine suppresses pathological vascular remodeling by inhibiting vascular smooth muscle cell proliferation and migration.
Cai Y, Knight WE, Guo S, Li JD, Knight PA, Yan C. Vinpocetine suppresses pathological vascular remodeling by inhibiting vascular smooth muscle cell proliferation and migration. J Pharmacol Exp Ther 2012, 343: 479-88. PMID: 22915768, DOI: 10.1124/jpet.112.195446.Peer-Reviewed Original ResearchThe short-time treatment with curcumin sufficiently decreases cell viability, induces apoptosis and copper enhances these effects in multidrug-resistant K562/A02 cells.
Lu JJ, Cai YJ, Ding J. The short-time treatment with curcumin sufficiently decreases cell viability, induces apoptosis and copper enhances these effects in multidrug-resistant K562/A02 cells. Mol Cell Biochem 2012, 360: 253-60. PMID: 21938404, DOI: 10.1007/s11010-011-1064-2.Peer-Reviewed Original Research
2011
Cyclic nucleotide phosphodiesterase 1 regulates lysosome-dependent type I collagen protein degradation in vascular smooth muscle cells.
Cai Y, Miller CL, Nagel DJ, Jeon KI, Lim S, Gao P, Knight PA, Yan C. Cyclic nucleotide phosphodiesterase 1 regulates lysosome-dependent type I collagen protein degradation in vascular smooth muscle cells. Arterioscler Thromb Vasc Biol 2011, 31: 616-23. PMID: 21148428, DOI: 10.1161/ATVBAHA.110.212621.Peer-Reviewed Original Research
2010
Ca2+/calmodulin-stimulated PDE1 regulates the beta-catenin/TCF signaling through PP2A B56 gamma subunit in proliferating vascular smooth muscle cells.
Jeon KI, Jono H, Miller CL, Cai Y, Lim S, Liu X, Gao P, Abe J, Li JD, Yan C. Ca2+/calmodulin-stimulated PDE1 regulates the beta-catenin/TCF signaling through PP2A B56 gamma subunit in proliferating vascular smooth muscle cells. FEBS J 2010, 277: 5026-39. PMID: 21078118, DOI: 10.1111/j.1742-4658.2010.07908.x.Peer-Reviewed Original Research
2009
Role of Ca2+/calmodulin-stimulated cyclic nucleotide phosphodiesterase 1 in mediating cardiomyocyte hypertrophy.
Miller CL, Oikawa M, Cai Y, Wojtovich AP, Nagel DJ, Xu X, Xu H, Florio V, Rybalkin SD, Beavo JA, Chen YF, Li JD, Blaxall BC, Abe J, Yan C. Role of Ca2+/calmodulin-stimulated cyclic nucleotide phosphodiesterase 1 in mediating cardiomyocyte hypertrophy. Circ Res 2009, 105: 956-64. PMID: 19797176, DOI: 10.1161/CIRCRESAHA.109.198515.Peer-Reviewed Original Research