2024
MECP2 directly interacts with RNA polymerase II to modulate transcription in human neurons
Liu Y, Flamier A, Bell G, Diao A, Whitfield T, Wang H, Wu Y, Schulte F, Friesen M, Guo R, Mitalipova M, Liu X, Vos S, Young R, Jaenisch R. MECP2 directly interacts with RNA polymerase II to modulate transcription in human neurons. Neuron 2024, 112: 1943-1958.e10. PMID: 38697112, DOI: 10.1016/j.neuron.2024.04.007.Peer-Reviewed Original ResearchPromoter-proximal regionRNA polymerase IIPolymerase IIHuman neuronsMethylated DNA binding protein MeCP2RNA Pol IIWild-typeLoss of gene activityAutism risk genesPol IIPositive cofactorCpG islandsTranscriptional regulationModulate transcriptionProtein MeCP2Patient mutationsNeurodevelopmental disorder Rett syndromeGene activationRisk genesProteomic analysisNeuronal genesRett syndromeGene expressionGenesRNA
1989
RNA Polymerase II Subunit RPB4 Is Essential for High- and Low-Temperature Yeast Cell Growth
Woychik N, Young R. RNA Polymerase II Subunit RPB4 Is Essential for High- and Low-Temperature Yeast Cell Growth. Molecular And Cellular Biology 1989, 9: 2854-2859. DOI: 10.1128/mcb.9.7.2854-2859.1989.Peer-Reviewed Original ResearchRPB4 subunitRNA polymerase II subunitEscherichia coli RNA polymeraseSequence analysis of tryptic peptidesAmino acid sequence analysis of tryptic peptidesRNA polymerase II activityAmino acid sequence analysisDeletion of RPB4Polymerase II subunitAnalysis of tryptic peptidesRNA polymerase IIAmino acidsPolymerase II activityRPB4 geneSigma subunitCrude extracts in vitroPolymerase IIRNA polymeraseSaccharomyces cerevisiaeEnzyme assemblyDNA sequencesRpb4Purified subunitsExtracts in vitroMRNA synthesis