2013
CFTR and V‐ATPase trafficking and function in the intestinal brush border membrane
Collaco A, Geibel P, Kravtsov D, Ghantaphang S, Lee B, Geibel J, Ameen N. CFTR and V‐ATPase trafficking and function in the intestinal brush border membrane. The FASEB Journal 2013, 27: 913.10-913.10. DOI: 10.1096/fasebj.27.1_supplement.913.10.Peer-Reviewed Original ResearchES cellsV-ATPaseMulti-subunit proton pumpCystic fibrosis transmembrane conductance regulator (CFTR) chloride channelV-ATPase traffickingIntestinal brush border membraneBrush border membraneBasolateral domainPlasma membraneVacuolar ATPaseE subunitBorder membraneCAMP stimulationProton pumpCAMP activationDistinct domainsFunctional synergyApical membraneCFTRChloride channelsProton effluxSubunitsMouse intestineTransportersE interaction
2008
S1771 Regulated Exocytosis and Fusion of Vesicle Associated CFTR to the Apical Plasma Membrane of the Intestine Is Mediated By the Snare Protein Syntaxin-3
Ameen N, Collaco A. S1771 Regulated Exocytosis and Fusion of Vesicle Associated CFTR to the Apical Plasma Membrane of the Intestine Is Mediated By the Snare Protein Syntaxin-3. Gastroenterology 2008, 134: a-266. DOI: 10.1016/s0016-5085(08)61239-1.Peer-Reviewed Original Research
2000
Subcellular distribution of CFTR in rat intestine supports a physiologic role for CFTR regulation by vesicle traffic
Ameen N, van Donselaar E, Posthuma G, de Jonge H, McLaughlin G, Geuze H, Marino C, Peters P. Subcellular distribution of CFTR in rat intestine supports a physiologic role for CFTR regulation by vesicle traffic. Histochemistry And Cell Biology 2000, 114: 219-228. PMID: 11083465, DOI: 10.1007/s004180000167.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorVesicle trafficSubcellular distributionVesicle insertionCAMP stimulationCAMP-activated chloride channelCryoimmunogold electron microscopyFibrosis transmembrane conductance regulatorApical plasma membraneTransmembrane conductance regulatorCultured intestinal cellsCFTR regulationCHE cellsPhysiologic roleVesicular compartmentsPlasma membraneApical redistributionConductance regulatorSubapical vesiclesCellular distributionChloride channelsSecretory cellsIntestinal cellsEpithelial cellsCells
1999
CFTR channel insertion to the apical surface in rat duodenal villus epithelial cells is upregulated by VIP in vivo
Ameen N, Martensson B, Bourguinon L, Marino C, Isenberg J, McLaughlin G. CFTR channel insertion to the apical surface in rat duodenal villus epithelial cells is upregulated by VIP in vivo. Journal Of Cell Science 1999, 112: 887-894. PMID: 10036238, DOI: 10.1242/jcs.112.6.887.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulator (CFTR) channelVillus epithelial cellsApical surfaceApical plasma membraneAnti-CFTR antibodiesQuantitative confocal microscopyNew protein synthesisApical membrane insertionIntestinal villus epithelial cellsEpithelial cellsMembrane CFTRMembrane insertionApical cytoskeletonNormal CFTRCHE cellsPlasma membraneAbsence of cycloheximideSubcellular redistributionCFTR channelsC-terminusCAMP stimulationIntracellular cAMP levelsProtein synthesisChannel insertionCFTR function