2011
Novel mechanisms of immune modulation of natalizumab in multiple sclerosis patients
Skarica M, Eckstein C, Whartenby K, Calabresi P. Novel mechanisms of immune modulation of natalizumab in multiple sclerosis patients. Journal Of Neuroimmunology 2011, 235: 70-76. PMID: 21550672, DOI: 10.1016/j.jneuroim.2011.02.010.Peer-Reviewed Original ResearchConceptsNatalizumab therapyPeripheral bloodMonth timepointProportion of CD20Proportion of CD4Number of CD14Multiple sclerosis patientsImmune cell compositionHematopoetic stem cellsPercentage of cellsMechanism of actionNatalizumab treatmentRRMS patientsMS patientsNK cellsSclerosis patientsTreatment onsetCytokine productionImmune compositionImmune modulationImmune cellsT cellsB cellsPatientsTherapy
2010
Dendritic Cells Transduced With Lentiviral Vectors Expressing VIP Differentiate Into VIP-secreting Tolerogenic-like DCs
Toscano M, Delgado M, Kong W, Martin F, Skarica M, Ganea D. Dendritic Cells Transduced With Lentiviral Vectors Expressing VIP Differentiate Into VIP-secreting Tolerogenic-like DCs. Molecular Therapy 2010, 18: 1035-1045. PMID: 20068554, PMCID: PMC2890107, DOI: 10.1038/mt.2009.293.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone Marrow CellsCell DifferentiationCell MovementCells, CulturedDendritic CellsEnzyme-Linked Immunosorbent AssayFlow CytometryGenetic VectorsLentivirusMiceMice, Inbred BALB CPolymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionTransduction, GeneticVasoactive Intestinal PeptideConceptsVasoactive intestinal peptideExperimental autoimmune encephalomyelitisDendritic cellsTherapeutic effectTolerogenic DCsVIP administrationInflammatory disordersT cellsImmune responseBone marrow-derived dendritic cellsBone marrow-derived DCsMarrow-derived dendritic cellsNeuropeptide vasoactive intestinal peptideTolerogenic dendritic cellsAllogeneic T cellsAutoimmune/inflammatory disordersRegulatory T cellsIL-10 productionProinflammatory cytokine secretionPromising therapeutic toolLentiviral vectorsHigh-dose inoculationCLP miceAutoimmune encephalomyelitisPuncture (CLP) sepsis
2009
Cyclophosphamide resets dendritic cell homeostasis and enhances antitumor immunity through effects that extend beyond regulatory T cell elimination
Radojcic V, Bezak K, Skarica M, Pletneva M, Yoshimura K, Schulick R, Luznik L. Cyclophosphamide resets dendritic cell homeostasis and enhances antitumor immunity through effects that extend beyond regulatory T cell elimination. Cancer Immunology, Immunotherapy 2009, 59: 137-148. PMID: 19590872, PMCID: PMC3103867, DOI: 10.1007/s00262-009-0734-3.Peer-Reviewed Original ResearchConceptsDendritic cell homeostasisAntitumor immunityT cellsLow-dose total body irradiationTumor antigen-specific T cellsAnti-CD25 monoclonal antibodyHematopoietic stem cell supportAntigen-specific T cellsUntreated tumor-bearing animalsTumor-infiltrating DCsExpansion of TregsRegulatory T cellsStem cell supportTotal body irradiationT cell responsesLess IL-10More IL-12Tumor-bearing animalsT cell eliminationCell homeostasisDC turnoverTreg depletionImmunotherapeutic strategiesIL-10Body irradiationSignal Transduction Inhibition of APCs Diminishes Th17 and Th1 Responses in Experimental Autoimmune Encephalomyelitis
Skarica M, Wang T, McCadden E, Kardian D, Calabresi P, Small D, Whartenby K. Signal Transduction Inhibition of APCs Diminishes Th17 and Th1 Responses in Experimental Autoimmune Encephalomyelitis. The Journal Of Immunology 2009, 182: 4192-4199. PMID: 19299717, PMCID: PMC3727416, DOI: 10.4049/jimmunol.0803631.Peer-Reviewed Original ResearchConceptsExperimental autoimmune encephalomyelitisPeripheral dendritic cellsDendritic cellsT cellsSignal transduction inhibitorsAutoimmune encephalomyelitisMultiple sclerosisIFN-gamma-secreting T cellsBone marrow chimera systemAg-specific T cellsTransduction inhibitorsT cell responsesT cell subsetsSignal transduction inhibitionCNS infiltrationCytokine polarizationIL-17IL-23Th1 responseAutoimmune responseCell subsetsCostimulatory moleculesIL-6TNF-alphaCEP-701
2007
Factors governing the activation of adoptively transferred donor T cells infused after allogeneic bone marrow transplantation in the mouse
Durakovic N, Radojcic V, Skarica M, Bezak K, Powell J, Fuchs E, Luznik L. Factors governing the activation of adoptively transferred donor T cells infused after allogeneic bone marrow transplantation in the mouse. Blood 2007, 109: 4564-4574. PMID: 17227829, PMCID: PMC1885486, DOI: 10.1182/blood-2006-09-048124.Peer-Reviewed Original ResearchConceptsDonor lymphocyte infusionDonor T cellsHost T cellsBone marrow transplantationT cellsHost chimerismHost APCsMarrow transplantationPrevious sensitizationAllogeneic bone marrow transplantationToll-like receptor ligandsGvH responseGVL reactivityLeukemia reactivityLymphocyte infusionMixed chimerismTLR ligandsHost antigensMurine modelVivo administrationCell activatorsHigh levelsAlloreactivityChimerismMHC
2006
Host-Derived Langerhans Cells Persist after MHC-Matched Allografting Independent of Donor T Cells and Critically Influence the Alloresponses Mediated by Donor Lymphocyte Infusions
Durakovic N, Bezak K, Skarica M, Radojcic V, Fuchs E, Murphy G, Luznik L. Host-Derived Langerhans Cells Persist after MHC-Matched Allografting Independent of Donor T Cells and Critically Influence the Alloresponses Mediated by Donor Lymphocyte Infusions. The Journal Of Immunology 2006, 177: 4414-4425. PMID: 16982876, DOI: 10.4049/jimmunol.177.7.4414.Peer-Reviewed Original ResearchConceptsCutaneous lymph nodesDonor lymphocyte infusionDonor T cellsBone marrow transplantationLangerhans cellsLymphocyte infusionMarrow transplantationCell reconstitutionT cellsDendritic cell reconstitutionDonor-derived DCsHost Langerhans cellsSplenic DC subsetsTLR7 ligand imiquimodMinor histocompatibility AgT-cell reconstitutionDLI administrationHost alloresponsesHost DCsLC emigrationDC subsetsLymph nodesCostimulatory moleculesHistocompatibility AgCells persist