2024
Dynamic biaxial loading of vascular smooth muscle cell seeded tissue equivalents
Paukner D, Jennings I, Cyron C, Humphrey J. Dynamic biaxial loading of vascular smooth muscle cell seeded tissue equivalents. Journal Of The Mechanical Behavior Of Biomedical Materials 2024, 157: 106639. PMID: 38970943, DOI: 10.1016/j.jmbbm.2024.106639.Peer-Reviewed Original ResearchCyclic loadingBiaxial loading conditionsPeriod of cyclic loadingStudy cell-matrix interactionsLoad amplitudeUniaxial experimentsDynamic loadingBiaxial forceCruciform samplesLoading conditionsExternal loadMechanical stateBoundary conditionsLoadExtracellular matrixInfluence cell phenotypeMechanical constraintsRange of forcesCell-matrix interactionsCollagen gelsTissue equivalentsForceSynthetic cellsLoad in vivoVascular smooth muscle cellsStiffening of the human proximal pulmonary artery with increasing age
Manning E, Mishall P, Ramachandra A, Hassab A, Lamy J, Peters D, Murphy T, Heerdt P, Singh I, Downie S, Choudhary G, Tellides G, Humphrey J. Stiffening of the human proximal pulmonary artery with increasing age. Physiological Reports 2024, 12: e16090. PMID: 38884325, PMCID: PMC11181131, DOI: 10.14814/phy2.16090.Peer-Reviewed Original ResearchConceptsProximal pulmonary arteriesPulmonary arteryAge-related stiffeningRight ventricular ejection fractionVentricular ejection fractionMean wall thicknessEjection fractionNo significant differenceSystemic circulationLuminal caliberDiffusing capacityArterial stiffeningIncreasing ageSignificant differenceDistensionArteryWall thicknessAdverse effectsWall strainVessel diameterCyclic biaxial strainOrgan donorsAgeMicrostructural remodelingCell signaling and tissue remodeling in the pulmonary autograft after the Ross procedure: A computational study
Maes L, Vervenne T, Hendrickx A, Estrada A, Van Hoof L, Verbrugghe P, Rega F, Jones E, Humphrey J, Famaey N. Cell signaling and tissue remodeling in the pulmonary autograft after the Ross procedure: A computational study. Journal Of Biomechanics 2024, 171: 112180. PMID: 38906711, DOI: 10.1016/j.jbiomech.2024.112180.Peer-Reviewed Original ResearchRoss procedurePulmonary autograftPatient's pulmonary valveTranscription factorsPulmonary valveSmooth muscleActivation of genesAortic positionRelevant transcription factorsTissue remodelingAutograftExcessive dilatationTranscriptome dataGene activationCell signalingSignaling pathwayTarget genesMultiscale modelGenesTissueCellsFailure mechanismCell-scaleMechanical propertiesCell-scale modelMultiscale computational model of aortic remodeling following postnatal disruption of TGFβ signaling
Estrada A, Irons L, Tellides G, Humphrey J. Multiscale computational model of aortic remodeling following postnatal disruption of TGFβ signaling. Journal Of Biomechanics 2024, 169: 112152. PMID: 38763809, PMCID: PMC11141772, DOI: 10.1016/j.jbiomech.2024.112152.Peer-Reviewed Original ResearchAdult aortaTGFB signalingSmooth muscle cellsAortic remodelingCardiac-inducedMouse modelNormal mechanical loadingMuscle cellsPostnatal developmentHemodynamic loadNormal loadAortaMechanical homeostasisMechanical loadingMultiscale computational modelIncreasing loadLoadCell signalingGene productsStructural integrityHemodynamics and Wall Mechanics of Vascular Graft Failure
Szafron J, Heng E, Boyd J, Humphrey J, Marsden A. Hemodynamics and Wall Mechanics of Vascular Graft Failure. Arteriosclerosis Thrombosis And Vascular Biology 2024, 44: 1065-1085. PMID: 38572650, PMCID: PMC11043008, DOI: 10.1161/atvbaha.123.318239.Peer-Reviewed Original ResearchConceptsVascular graftsTissue-engineered vascular graftsWall mechanicsSolid mechanicsVascular graft failureLoad magnitudeMechanobiological processesLoadMechanobiological stimuliMechanosensitive signaling pathwaysBiomechanical stateWallGraft failureBiomechanical loadingCongenital heart surgeryCoronary artery bypass graftingEnd-organ dysfunctionGraft materialArtery bypass graftingFeedback loopComputational toolsRemodeling of Murine Branch Pulmonary Arteries Under Chronic Hypoxia and Short-Term Normoxic Recovery.
Ramachandra A, Jiang B, Jennings I, Manning E, Humphrey J. Remodeling of Murine Branch Pulmonary Arteries Under Chronic Hypoxia and Short-Term Normoxic Recovery. Journal Of Biomechanical Engineering 2024, 146 PMID: 38421341, DOI: 10.1115/1.4064967.Peer-Reviewed Original ResearchWeeks of hypoxiaBranch pulmonary arteriesWeeks of normoxic recoveryPulmonary arteryEndothelial cell responsesSmooth muscleChronic hypoxiaNormoxic recoveryPulmonary arterial remodelingCell responsesProximal pulmonary arteriesLongitudinal changesHypoxia-induced changesC57BL/6J micePulmonary pathologyThin collagen fibersArterial remodelingBiaxial mechanicsCircumferential stressEnergy storageNormal valuesHypoxiaWeeksVascular adaptationFraction of cytoplasmTissue engineered vascular grafts are resistant to the formation of dystrophic calcification
Turner M, Blum K, Watanabe T, Schwarz E, Nabavinia M, Leland J, Villarreal D, Schwartzman W, Chou T, Baker P, Matsumura G, Krishnamurthy R, Yates A, Hor K, Humphrey J, Marsden A, Stacy M, Shinoka T, Breuer C. Tissue engineered vascular grafts are resistant to the formation of dystrophic calcification. Nature Communications 2024, 15: 2187. PMID: 38467617, PMCID: PMC10928115, DOI: 10.1038/s41467-024-46431-4.Peer-Reviewed Original ResearchConceptsTissue-engineered vascular graftsTissue engineered vascular graftsVascular graftsCongenital heart surgeryComputational fluid dynamics simulationsFluid dynamics simulationsPolytetrafluoroethylene graftHeart surgeryDystrophic calcificationRetrospective clinical studyGraft complianceDegree of calcificationClinical studiesProsthetic biomaterialsBiomaterialsAnimal modelsPolytetrafluoroethyleneHemodynamic performanceNatural historyCalcificationGraftOvine modelVascular conduitsLong-term successSurgeryGrand Challenges at the Interface of Engineering and Medicine
Subramaniam S, Akay M, Anastasio M, Bailey V, Boas D, Bonato P, Chilkoti A, Cochran J, Colvin V, Desai T, Duncan J, Epstein F, Fraley S, Giachelli C, Grande-Allen K, Green J, Guo X, Hilton I, Humphrey J, Johnson C, Karniadakis G, King M, Kirsch R, Kumar S, Laurencin C, Li S, Lieber R, Lovell N, Mali P, Margulies S, Meaney D, Ogle B, Palsson B, Peppas N, Perreault E, Rabbitt R, Setton L, Shea L, Shroff S, Shung K, Tolias A, van der Meulen M, Varghese S, Vunjak-Novakovic G, White J, Winslow R, Zhang J, Zhang K, Zukoski C, Miller M. Grand Challenges at the Interface of Engineering and Medicine. IEEE Open Journal Of Engineering In Medicine And Biology 2024, 5: 1-13. PMID: 38415197, PMCID: PMC10896418, DOI: 10.1109/ojemb.2024.3351717.Peer-Reviewed Original ResearchCentral Artery Hemodynamics in Angiotensin II-Induced Hypertension and Effects of Anesthesia
Hopper S, Weiss D, Mikush N, Jiang B, Spronck B, Cavinato C, Humphrey J, Figueroa C. Central Artery Hemodynamics in Angiotensin II-Induced Hypertension and Effects of Anesthesia. Annals Of Biomedical Engineering 2024, 52: 1051-1066. PMID: 38383871, DOI: 10.1007/s10439-024-03440-0.Peer-Reviewed Original ResearchEffects of angiotensin II infusionMouse-to-mouse variationAngiotensin II-induced hypertensionRegional vascular structuresII-induced hypertensionAngiotensin II-infused miceAngiotensin II infusionWild-type miceDescending thoracic aortaGroups of miceSuprarenal abdominal aortaEffects of hypertensionCentral arterial stiffnessDepressed hemodynamicsII infusionIndicator of hypertensionSystemic hypertensionRenovascular diseaseCardiac functionInduced hypertensionDistal aortaThoracic aortaEffects of anesthesiaMouse modelAbdominal aortaDevelopmental changes in lung function of mice are independent of sex as a biological variable
Bärnthaler T, Ramachandra A, Ebanks S, Guerrera N, Sharma L, Dela Cruz C, Humphrey J, Manning E. Developmental changes in lung function of mice are independent of sex as a biological variable. American Journal Of Physiology - Lung Cellular And Molecular Physiology 2024, 326: l627-l637. PMID: 38375577, DOI: 10.1152/ajplung.00120.2023.Peer-Reviewed Original ResearchPulmonary function testsWeeks of ageStatic complianceMale miceInspiratory capacityLung functionBody weight-basedHuman lung pathologiesLung function of miceFemale C57BL/6J miceLung function parametersSex-dependent changesFunction of miceMeasures of lung functionIndependent of sexPreclinical modelsFemale miceC57BL/6J miceLung pathologyFunction testsMiceWeight-basedLungLung disease researchWeeksA Systematic Comparison of Normal Structure and Function of the Greater Thoracic Vessels
Ramachandra A, Cavinato C, Humphrey J. A Systematic Comparison of Normal Structure and Function of the Greater Thoracic Vessels. Annals Of Biomedical Engineering 2024, 52: 958-966. PMID: 38227167, DOI: 10.1007/s10439-023-03432-6.Peer-Reviewed Original ResearchThoracic vesselsVena cavaThoracic aortaCongenital heart surgeryRight pulmonary arterySuperior vena cavaInferior vena cavaRight subclavian arteryThoracic inferior vena cavaWild-type miceAscending thoracic aortaDescending thoracic aortaPulmonary arterySubclavian arteryHeart surgerySurgical interventionVasoactive responsesVenous circulationThoracicCavaNormal structureAortaArteryBiomechanical propertiesVascular systemChapter 25 Pharmacological treatments, mouse models, and the aorta
Humphrey J. Chapter 25 Pharmacological treatments, mouse models, and the aorta. 2024, 569-592. DOI: 10.1016/b978-0-323-95484-6.00021-x.Peer-Reviewed Original Research
2023
Multiscale insights into postnatal aortic development
Rego B, Murtada S, Li G, Tellides G, Humphrey J. Multiscale insights into postnatal aortic development. Biomechanics And Modeling In Mechanobiology 2023, 23: 687-701. PMID: 38151614, DOI: 10.1007/s10237-023-01800-8.Peer-Reviewed Original ResearchTempol improves aortic mechanics in a mouse model of hypertension
Niestrawska J, Spronck B, Cavinato C, Humphrey J. Tempol improves aortic mechanics in a mouse model of hypertension. Journal Of Biomechanics 2023, 162: 111911. PMID: 38150954, PMCID: PMC10896091, DOI: 10.1016/j.jbiomech.2023.111911.Peer-Reviewed Original Research
2019
Computational modeling predicts immuno-mechanical mechanisms of maladaptive aortic remodeling in hypertension
Latorre M, Bersi MR, Humphrey JD. Computational modeling predicts immuno-mechanical mechanisms of maladaptive aortic remodeling in hypertension. International Journal Of Engineering Science 2019, 141: 35-46. PMID: 32831391, PMCID: PMC7437922, DOI: 10.1016/j.ijengsci.2019.05.014.Peer-Reviewed Original ResearchAortic remodelingBlood pressure elevationCentral artery stiffnessMajor risk factorCommon mouse modelsBasic science studiesUncontrolled hypertensionIndicators of diseaseAortic stiffeningPressure elevationAdventitial fibrosisArtery stiffnessAortic growthRisk factorsAbdominal aortaCardiovascular diseaseMouse modelTherapeutic strategiesHypertensionAortic wallCompensatory increaseInflammationFibrosisDiseaseMarked increase
2018
Strongly Coupled Morphological Features of Aortic Aneurysms Drive Intraluminal Thrombus
Bhagavan D, Di Achille P, Humphrey JD. Strongly Coupled Morphological Features of Aortic Aneurysms Drive Intraluminal Thrombus. Scientific Reports 2018, 8: 13273. PMID: 30185838, PMCID: PMC6125404, DOI: 10.1038/s41598-018-31637-6.Peer-Reviewed Original ResearchCritical roles of time-scales in soft tissue growth and remodeling
Latorre M, Humphrey JD. Critical roles of time-scales in soft tissue growth and remodeling. APL Bioengineering 2018, 2: 026108. PMID: 31069305, PMCID: PMC6324203, DOI: 10.1063/1.5017842.Peer-Reviewed Original ResearchCompromised mechanical homeostasis in arterial aging and associated cardiovascular consequences
Ferruzzi J, Madziva D, Caulk AW, Tellides G, Humphrey JD. Compromised mechanical homeostasis in arterial aging and associated cardiovascular consequences. Biomechanics And Modeling In Mechanobiology 2018, 17: 1281-1295. PMID: 29754316, PMCID: PMC8344131, DOI: 10.1007/s10237-018-1026-7.Peer-Reviewed Original ResearchMeSH KeywordsAgingAnimalsAorta, AbdominalAorta, ThoracicBiomechanical PhenomenaCardiovascular DiseasesCardiovascular SystemCarotid ArteriesCarotid Artery, CommonDiastoleDisease Models, AnimalElastinExtracellular Matrix ProteinsHemodynamicsHomeostasisHumansMaleMiceModels, CardiovascularPressureRecombinant ProteinsStress, MechanicalTime FactorsVascular StiffnessConceptsCentral arteriesMale wild-type miceArterial wallAberrant matrix remodelingCentral pulse pressureInfrarenal abdominal aortaCommon carotid arteryWild-type miceDiastolic functionCardiovascular consequencesCardiac massHemodynamic sequelaePulse pressureAbdominal aortaThoracic aortaArterial agingVascular agingCommon findingTreatment strategiesCarotid arteryArteryLeft ventricleCarotid vesselsCentral vesselsVivo axial stretch
2017
mTOR (Mechanistic Target of Rapamycin) Inhibition Decreases Mechanosignaling, Collagen Accumulation, and Stiffening of the Thoracic Aorta in Elastin-Deficient Mice
Jiao Y, Li G, Li Q, Ali R, Qin L, Li W, Qyang Y, Greif DM, Geirsson A, Humphrey JD, Tellides G. mTOR (Mechanistic Target of Rapamycin) Inhibition Decreases Mechanosignaling, Collagen Accumulation, and Stiffening of the Thoracic Aorta in Elastin-Deficient Mice. Arteriosclerosis Thrombosis And Vascular Biology 2017, 37: 1657-1666. PMID: 28751568, PMCID: PMC5574180, DOI: 10.1161/atvbaha.117.309653.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAorta, ThoracicAortic DiseasesCell ProliferationCollagenElastinEverolimusFocal Adhesion Kinase 1Genetic Predisposition to DiseaseHumansImatinib MesylateMechanistic Target of Rapamycin Complex 1Mechanistic Target of Rapamycin Complex 2Mechanotransduction, CellularMice, Inbred C57BLMice, KnockoutMultiprotein ComplexesMuscle, Smooth, VascularPhenotypePhosphorylationProtein Kinase InhibitorsSirolimusTime FactorsTOR Serine-Threonine KinasesVascular StiffnessWilliams SyndromeConceptsElastin deficiencyCollagen accumulationArterial phenotypeNull miceGrowth factorSmooth muscle cell proliferationMuscle cell proliferationEarly postnatal deathInhibition of mTORAortic fibrosisAortic obstructionMedial thickeningAortic stiffeningNeonatal deathLuminal stenosisPharmacological blockadeAbsence of elastinThoracic aortaTherapeutic benefitJuvenile micePostnatal deathMTOR inhibitionAortaHeterozygous lossMiceComparison of 10 murine models reveals a distinct biomechanical phenotype in thoracic aortic aneurysms
Bellini C, Bersi MR, Caulk AW, Ferruzzi J, Milewicz DM, Ramirez F, Rifkin DB, Tellides G, Yanagisawa H, Humphrey JD. Comparison of 10 murine models reveals a distinct biomechanical phenotype in thoracic aortic aneurysms. Journal Of The Royal Society Interface 2017, 14: 20161036. PMID: 28490606, PMCID: PMC5454287, DOI: 10.1098/rsif.2016.1036.Peer-Reviewed Original ResearchConceptsGenetic mutationsExtracellular matrix proteinsTransmembrane receptorsCytoskeletal proteinsMatrix proteinsWild-type controlsBiomechanical phenotypeDysfunctional mechanosensingExtracellular matrixDiverse mouse modelsSmooth muscle cellsMutationsMuscle cellsProteinAorta of miceMurine modelCellsMechanosensingElastic fiber integrityMouse modelMechanoregulationStructural integrityPhenotypeIntracellularIntegrity