2024
Stiffening of the human proximal pulmonary artery with increasing age
Manning E, Mishall P, Ramachandra A, Hassab A, Lamy J, Peters D, Murphy T, Heerdt P, Singh I, Downie S, Choudhary G, Tellides G, Humphrey J. Stiffening of the human proximal pulmonary artery with increasing age. Physiological Reports 2024, 12: e16090. PMID: 38884325, PMCID: PMC11181131, DOI: 10.14814/phy2.16090.Peer-Reviewed Original ResearchConceptsProximal pulmonary arteriesPulmonary arteryAge-related stiffeningRight ventricular ejection fractionVentricular ejection fractionMean wall thicknessEjection fractionNo significant differenceSystemic circulationLuminal caliberDiffusing capacityArterial stiffeningIncreasing ageSignificant differenceDistensionArteryWall thicknessAdverse effectsWall strainVessel diameterCyclic biaxial strainOrgan donorsAgeMicrostructural remodeling
2023
Alveolar Vascular Remodeling in Nonspecific Interstitial Pneumonia: Replacement of Normal Lung Capillaries with COL15A1-Positive Endothelial Cells.
Schupp J, Manning E, Chioccioli M, Kamp J, Christian L, Ryu C, Herzog E, Kühnel M, Prasse A, Kaminski N, Jonigk D, Homer R, Neubert L, Ius F, stringJustet A, Hariri L, Seeliger B, Welte T, Knipe R, Gottlieb J. Alveolar Vascular Remodeling in Nonspecific Interstitial Pneumonia: Replacement of Normal Lung Capillaries with COL15A1-Positive Endothelial Cells. American Journal Of Respiratory And Critical Care Medicine 2023, 208: 819-822. PMID: 37552025, PMCID: PMC10563189, DOI: 10.1164/rccm.202303-0544le.Peer-Reviewed Original ResearchVISTA (PD-1H) Is a Crucial Immune Regulator to Limit Pulmonary Fibrosis.
Kim S, Adams T, Hu Q, Shin H, Chae G, Lee S, Sharma L, Kwon H, Lee F, Park H, Huh W, Manning E, Kaminski N, Sauler M, Chen L, Song J, Kim T, Kang M. VISTA (PD-1H) Is a Crucial Immune Regulator to Limit Pulmonary Fibrosis. American Journal Of Respiratory Cell And Molecular Biology 2023, 69: 22-33. PMID: 36450109, PMCID: PMC10324045, DOI: 10.1165/rcmb.2022-0219oc.Peer-Reviewed Original ResearchConceptsIdiopathic pulmonary fibrosisPulmonary fibrosisImmune regulatorsTherapeutic potentialHuman idiopathic pulmonary fibrosisCrucial immune regulatorsNovel immune regulatorPulmonary fibrosis micePulmonary fibrosis modelNovel therapeutic targetRole of VISTAWild-type littermatesMonocyte-derived macrophagesT lymphocyte lineageVISTA expressionIPF treatmentAntibody treatmentImmune landscapeFibrotic mediatorsLung fibrosisFibrosis miceInflammatory responseFibrosis modelMyeloid populationsTherapeutic target
2022
Epidermal Growth Factor Receptor Inhibition Is Protective in Hyperoxia‐Induced Lung Injury
Harris ZM, Sun Y, Joerns J, Clark B, Hu B, Korde A, Sharma L, Shin HJ, Manning EP, Placek L, Unutmaz D, Stanley G, Chun H, Sauler M, Rajagopalan G, Zhang X, Kang MJ, Koff JL. Epidermal Growth Factor Receptor Inhibition Is Protective in Hyperoxia‐Induced Lung Injury. Oxidative Medicine And Cellular Longevity 2022, 2022: 9518592. PMID: 36193076, PMCID: PMC9526641, DOI: 10.1155/2022/9518592.Peer-Reviewed Original ResearchConceptsAcute lung injuryEpidermal growth factor receptorAlveolar epithelial cellsLung injurySevere hyperoxiaEGFR inhibitionEpithelial cellsHyperoxia-Induced Lung InjuryRole of EGFRMurine alveolar epithelial cellsGrowth factor receptor inhibitionWorse clinical outcomesEpidermal growth factor receptor inhibitionHuman alveolar epithelial cellsWild-type littermatesPoly (ADP-ribose) polymeraseTerminal dUTP nickGrowth factor receptorClinical outcomesImproved survivalReceptor inhibitionLung repairProtective roleComplex roleEGFR deletion
2021
Integrated Single-Cell Atlas of Endothelial Cells of the Human Lung
Schupp JC, Adams TS, Cosme C, Raredon MSB, Yuan Y, Omote N, Poli S, Chioccioli M, Rose KA, Manning EP, Sauler M, DeIuliis G, Ahangari F, Neumark N, Habermann AC, Gutierrez AJ, Bui LT, Lafyatis R, Pierce RW, Meyer KB, Nawijn MC, Teichmann SA, Banovich NE, Kropski JA, Niklason LE, Pe’er D, Yan X, Homer RJ, Rosas IO, Kaminski N. Integrated Single-Cell Atlas of Endothelial Cells of the Human Lung. Circulation 2021, 144: 286-302. PMID: 34030460, PMCID: PMC8300155, DOI: 10.1161/circulationaha.120.052318.Peer-Reviewed Original ResearchConceptsDifferential expression analysisPrimary lung endothelial cellsLung endothelial cellsCell typesMarker genesExpression analysisSingle-cell RNA sequencing dataCross-species analysisVenous endothelial cellsEndothelial marker genesSingle-cell atlasMarker gene setsRNA sequencing dataEndothelial cellsSubsequent differential expression analysisDifferent lung cell typesResident cell typesLung cell typesCellular diversityEndothelial cell typesCapillary endothelial cellsHuman lung endothelial cellsPhenotypic diversityEndothelial diversityIndistinguishable populationsDisinfection of Pseudomonas aeruginosa from N95 respirators with ozone: a pilot study
Manning EP, Stephens MD, Dufresne S, Silver B, Gerbarg P, Gerbarg Z, Dela Cruz CS, Sharma L. Disinfection of Pseudomonas aeruginosa from N95 respirators with ozone: a pilot study. BMJ Open Respiratory Research 2021, 8: e000781. PMID: 33431507, PMCID: PMC7802653, DOI: 10.1136/bmjresp-2020-000781.Peer-Reviewed Original Research
2019
Plasma mitochondrial DNA is associated with extrapulmonary sarcoidosis
Ryu C, Brandsdorfer C, Adams T, Hu B, Kelleher DW, Yaggi M, Manning EP, Walia A, Reeves B, Pan H, Winkler J, Minasyan M, Dela Cruz CS, Kaminski N, Gulati M, Herzog EL. Plasma mitochondrial DNA is associated with extrapulmonary sarcoidosis. European Respiratory Journal 2019, 54: 1801762. PMID: 31273041, PMCID: PMC8088542, DOI: 10.1183/13993003.01762-2018.Peer-Reviewed Original ResearchConceptsExtrapulmonary diseaseMitochondrial DNAExtracellular mtDNABAL fluidAlpha-1 antitrypsin deficiencyPlasma mitochondrial DNAPlasma of patientsAfrican AmericansExtrapulmonary sarcoidosisSarcoidosis cohortSarcoidosis subjectsScadding stageAfrican American descentClinical featuresClinical findingsGranulomatous diseaseHealthy controlsAntitrypsin deficiencyGenomic researchHigher oddsSarcoidosisAggressive phenotypeMechanistic basisDiseaseTherapeutic insightsNew Applications of Old Drugs as Novel Therapies in Idiopathic Pulmonary Fibrosis. Metformin, Hydroxychloroquine, and Thyroid Hormone
Manning EP, Losier A, Emeagwali N, Ryu C, Honiden S. New Applications of Old Drugs as Novel Therapies in Idiopathic Pulmonary Fibrosis. Metformin, Hydroxychloroquine, and Thyroid Hormone. American Journal Of Respiratory And Critical Care Medicine 2019, 199: 1561-1563. PMID: 30822095, PMCID: PMC7051474, DOI: 10.1164/rccm.201809-1700rr.Peer-Reviewed Original ResearchPulmonary artery pseudoaneurysm causing massive hemoptysis in hyperimmunoglobulin E syndrome: a case report
Hakim A, Bazan IS, Sanogo ML, Manning EP, Pollak JS, Chupp GL. Pulmonary artery pseudoaneurysm causing massive hemoptysis in hyperimmunoglobulin E syndrome: a case report. BMC Pulmonary Medicine 2019, 19: 34. PMID: 30736787, PMCID: PMC6368789, DOI: 10.1186/s12890-019-0797-7.Peer-Reviewed Original ResearchConceptsPulmonary artery pseudoaneurysmArtery pseudoaneurysmMassive hemoptysisArtery angiographyE syndromeSelective pulmonary artery angiographyRare primary immunodeficiency disorderBronchial artery angiographyImmunoglobulin E titersRecurrent skin abscessesRecurrent respiratory infectionsAllergic bronchopulmonary aspergillosisHyperimmunoglobulin E syndromePulmonary artery angiographyPrimary immunodeficiency disordersRecurrent massive hemoptysisBronchopulmonary aspergillosisActive hemorrhageBronchial arteryRespiratory infectionsTranscatheter embolizationCase presentationWeSkin abscessesCase reportImmunodeficiency disorders
2018
Early and prolonged opportunities to practice suturing increases medical student comfort with suturing during clerkships: Suturing during cadaver dissection
Manning EP, Mishall PL, Weidmann MD, Flax H, Lan S, Erlich M, Burton WB, Olson TR, Downie SA. Early and prolonged opportunities to practice suturing increases medical student comfort with suturing during clerkships: Suturing during cadaver dissection. Anatomical Sciences Education 2018, 11: 605-612. PMID: 29603672, PMCID: PMC6165707, DOI: 10.1002/ase.1785.Peer-Reviewed Original ResearchConceptsThird-year clerkshipMedical studentsStudent confidenceFirst-year medical studentsMedical student comfortProlonged opportunityLack of instructionAnatomy dissection laboratoryStudent behaviorStudents' attitudesStudent comfortDissection laboratoryProcedural skillsStudentsClerkshipFirst-time exposureProlonged introductionPatient interactionsCadaver dissectionLow-stress environmentConfidenceOpportunitiesInstructionSkillsAttitudes
2016
Central Nervous System Oxygen Toxicity and Hyperbaric Oxygen Seizures
Manning EP. Central Nervous System Oxygen Toxicity and Hyperbaric Oxygen Seizures. Aerospace Medicine And Human Performance 2016, 87: 477-486. PMID: 27099087, PMCID: PMC7092644, DOI: 10.3357/amhp.4463.2016.Peer-Reviewed Original ResearchConceptsCNS O2 toxicityO2 toxicityCentral nervous system O2 toxicityCentral nervous system oxygen toxicityHyperbaric O2 treatmentTonic-clonic seizuresOxygen seizuresMulticenter trialHyperbaric oxygenNumerous physiological changesTherapeutic agentsOxygen toxicityElevated PO2Scientific evidenceFurther studiesNumerous endpointsSeizuresProtective reactionsPhysiological changesToxicity
2014
Young Woman With Epigastric Pain and Vomiting
Manning EP, Laskowski LK, Nelson LS, Touger M. Young Woman With Epigastric Pain and Vomiting. Annals Of Emergency Medicine 2014, 64: 330-333. PMID: 25149968, PMCID: PMC4203436, DOI: 10.1016/j.annemergmed.2013.12.022.Peer-Reviewed Original Research
2012
Molecular Effects of Familial Hypertrophic Cardiomyopathy-Related Mutations in the TNT1 Domain of cTnT
Manning EP, Tardiff JC, Schwartz SD. Molecular Effects of Familial Hypertrophic Cardiomyopathy-Related Mutations in the TNT1 Domain of cTnT. Journal Of Molecular Biology 2012, 421: 54-66. PMID: 22579624, PMCID: PMC3545441, DOI: 10.1016/j.jmb.2012.05.008.Peer-Reviewed Original ResearchCorrelation of Molecular and Functional Effects of Mutations in Cardiac Troponin T Linked to Familial Hypertrophic Cardiomyopathy
Manning EP, Guinto PJ, Tardiff JC. Correlation of Molecular and Functional Effects of Mutations in Cardiac Troponin T Linked to Familial Hypertrophic Cardiomyopathy. Journal Of Biological Chemistry 2012, 287: 14515-14523. PMID: 22334656, PMCID: PMC3340284, DOI: 10.1074/jbc.m111.257436.Peer-Reviewed Original Research
2011
A Model of Calcium Activation of the Cardiac Thin Filament
Manning EP, Tardiff JC, Schwartz SD. A Model of Calcium Activation of the Cardiac Thin Filament. Biochemistry 2011, 50: 7405-7413. PMID: 21797264, PMCID: PMC3165030, DOI: 10.1021/bi200506k.Peer-Reviewed Original Research