2019
Keratinocyte-derived CCL20 orchestrates epidermal localization of IL-17-producing γδ T cells and ILCs to mediate psoriasis-like skin inflammation
Singh T, Lu X, Singh S, Zhang H, Doucet M, Kominsky S, Farber J. Keratinocyte-derived CCL20 orchestrates epidermal localization of IL-17-producing γδ T cells and ILCs to mediate psoriasis-like skin inflammation. The Journal Of Immunology 2019, 202: 183.21-183.21. DOI: 10.4049/jimmunol.202.supp.183.21.Peer-Reviewed Original ResearchIL-17-producingT cellsKnock-in miceIL-17Psoriasis-like skin inflammationGd T cellsExpression of CCL20Expression of IL17Hair folliclesCCR6 ligandIMQ treatmentPsoriatic dermatitisCCR6IL-22Skin inflammationReduce inflammationCCL20Day 4Treated skinInflammationMiceB-defensinsSuperficial epidermisKeratinocytesIMQ
2017
Single-cell analysis of CCR6+ human Th cells reveals varying degrees of the type-17 phenotype, ranging from cells co-expressing to those fully suppressing opposing pathways
Singh S, Edara N, Zhang H, Chen J, Farber J. Single-cell analysis of CCR6+ human Th cells reveals varying degrees of the type-17 phenotype, ranging from cells co-expressing to those fully suppressing opposing pathways. The Journal Of Immunology 2017, 198: 150.14-150.14. DOI: 10.4049/jimmunol.198.supp.150.14.Peer-Reviewed Original ResearchType 17Co-expressionLineage-specific genesCo-expressed genesCells expressing various levelsTh cellsExpression of genesPattern of expressionSingle-cell analysisExpression of CCR6Opposing pathwaysMulti-lineage cellsGenesHuman Th cellsCells co-expressingLineagesDifferentiation in vivoCell sortingCCR6 expressionExpression levelsMemory populationIL-22IL-17A/FRT-PCRCCR6NOD2 deficiency exacerbates imiquimod-induced, psoriasis-like dermatitis
Singh T, Cipolla E, Singh S, Farber J. NOD2 deficiency exacerbates imiquimod-induced, psoriasis-like dermatitis. The Journal Of Immunology 2017, 198: 75.17-75.17. DOI: 10.4049/jimmunol.198.supp.75.17.Peer-Reviewed Original ResearchNucleotide-binding oligomerization domainNod2-/- micePsoriasis-like dermatitisToll-like receptorsIL-22Levels of mRNAPattern recognition proteinsIL-17AUp-regulation of NOD2Inhibition of Toll-like receptorImiquimod-treated skinRecognition proteinsDecreased levels of mRNAOligomerization domainAutoimmune skin diseaseIncreased levels of mRNAWild-type controlsExpression of mRNAInactivating mutationsAntibacterial defenseImiquimod-inducedNod2 deficiencyNOD2 functionT cellsIL-1ra
2014
Blood monocytes expressing CCR6 and monocyte-derived dendritic cells in dermis and epidermis are critical for IL-23-induced psoriasis-like inflammation in mice (CAM5P.238)
Singh T, Zhang H, Farber J. Blood monocytes expressing CCR6 and monocyte-derived dendritic cells in dermis and epidermis are critical for IL-23-induced psoriasis-like inflammation in mice (CAM5P.238). The Journal Of Immunology 2014, 192: 180.9-180.9. DOI: 10.4049/jimmunol.192.supp.180.9.Peer-Reviewed Original ResearchCCR6-/- miceIL-23 injectionDendritic cellsPsoriasis-like inflammationIL-23Blood monocytesAccumulation of dendritic cellsMonocyte-derived dendritic cellsHuman studiesLangerin-DTR micePsoriasis-like pathologyExpression of CCR6Monocyte-derived cellsLangerin+ cellsInflamed skinMyeloid cellsNon-monocyticIL-22Injected i.Psoriatic skinCCR6InflammationMiceMouse skinMonocytes
2010
Exaggerated IL-23-induced psoriasis-like inflammation in mice lacking CCR4 (135.32)
Hedrick M, Zhang H, Farber J. Exaggerated IL-23-induced psoriasis-like inflammation in mice lacking CCR4 (135.32). The Journal Of Immunology 2010, 184: 135.32-135.32. DOI: 10.4049/jimmunol.184.supp.135.32.Peer-Reviewed Original ResearchCCR4-deficient micePsoriasis-like inflammationT cellsIL-23Deficient miceIL-22Intradermal injection of IL-23Foxp3+ cellsExaggerated inflammatory responseWild-type miceIL-10 family cytokinesPro-inflammatory cytokinesIntramural Research ProgramIL-10 familyTh17 cellsTh2 cellsDendritic cellsIL-17AIL-17FMicroabscess formationChemokine receptorsInjected skinInfiltrating neutrophilsEffector functionsIntradermal injection