2022
Super-resolved fluorescence imaging of peripheral nerve
Hernández I, Mohan S, Minderler S, Jowett N. Super-resolved fluorescence imaging of peripheral nerve. Scientific Reports 2022, 12: 12450. PMID: 35864187, PMCID: PMC9304405, DOI: 10.1038/s41598-022-16769-0.Peer-Reviewed Original ResearchConceptsSuper-resolution microscopySuper-resolution imagingNanoscale resolutionSuper-resolution imaging techniquesEmission depletion (STED) microscopyNanoscale cellular structuresStructured illumination microscopyElectron microscopyVisible lightDiffraction limited resolutionDepletion microscopyMicroscopyFluorescence imagingNon-toxic meansIllumination microscopyBiomedical sciencesFluorescent dyeBrightfield microscopyFluorescent objectsSample preparationFluctuation microscopy
2020
Considerations in Management of Acute Otitis Media in the COVID-19 Era
Mohan S, Workman A, Barshak M, Welling D, Abdul-Aziz D. Considerations in Management of Acute Otitis Media in the COVID-19 Era. Annals Of Otology Rhinology & Laryngology 2020, 130: 520-527. PMID: 32911957, DOI: 10.1177/0003489420958443.Peer-Reviewed Original ResearchConceptsMiddle ear fluidAcute otitis mediaComplicated acute otitis mediaEar fluidOtitis mediaSARS-CoV-2 infectionSARS-CoV-2Management of Acute Otitis MediaFacial paralysisPCR of nasopharyngeal swabClinical management of patientsPositive viral PCRTreated with myringotomyManagement of patientsBlue light filterCadaveric temporal bonesOtologic findingsSystemic antibioticsEar diseaseNegative PCRAcute infectionClinical managementOtologic proceduresProlonged close contactNasopharyngeal swabsLabel‐free histomorphometry of peripheral nerve by stimulated Raman spectroscopy
Hernández I, Yang W, Mohan S, Jowett N. Label‐free histomorphometry of peripheral nerve by stimulated Raman spectroscopy. Muscle & Nerve 2020, 62: 137-142. PMID: 32304246, DOI: 10.1002/mus.26895.Peer-Reviewed Original ResearchToward an Automatic System for Computer-Aided Assessment in Facial Palsy
Guarin D, Yunusova Y, Taati B, Dusseldorp J, Mohan S, Tavares J, van Veen M, Fortier E, Hadlock T, Jowett N. Toward an Automatic System for Computer-Aided Assessment in Facial Palsy. Facial Plastic Surgery & Aesthetic Medicine 2020, 22: 42-49. PMID: 32053425, PMCID: PMC7362997, DOI: 10.1089/fpsam.2019.29000.gua.Peer-Reviewed Original ResearchConceptsFacial landmark localizationLandmark localizationMachine learningML algorithmsFacial landmarksAutomatic systemComputer-aided diagnosis systemLocalization accuracyGraphical user interfaceCustom graphical user interfaceLandmark localization accuracyComputer-Aided AssessmentPoor localization accuracyUser interfaceDetection modelMore imagesDiagnosis systemML modelsAvailable algorithmsManual markingNovel ML algorithmsNovel ML modelsLarge databaseAlgorithm accuracyLocalization model
2019
An Anatomic Variant of the Ansa Cervicalis Precluding Its Use as a Donor Nerve
Zhu A, Mohan S, Richmon J, Jowett N. An Anatomic Variant of the Ansa Cervicalis Precluding Its Use as a Donor Nerve. Annals Of Otology Rhinology & Laryngology 2019, 129: 78-81. PMID: 31510759, DOI: 10.1177/0003489419875975.Peer-Reviewed Original ResearchConceptsAnsa cervicalisAnatomic variantsVagus nerveParotid adenoid cystic carcinomaLeft vocal fold palsyCranial nerve repairFacial nerve resectionIpsilateral hypoglossal nerveRelevant anatomic variantsVocal fold palsyAdenoid cystic carcinomaInterposition repairDonor nerveNerve resectionSural nerveHypoglossal nerveInadvertent injuryNerve epineuriumCystic carcinomaGraft harvestNerve repairApparent morbidityNerveMuscle contractionCervicalis
2018
Outcome Tracking in Facial Palsy
Dusseldorp J, van Veen M, Mohan S, Hadlock T. Outcome Tracking in Facial Palsy. Otolaryngologic Clinics Of North America 2018, 51: 1033-1050. PMID: 30172554, DOI: 10.1016/j.otc.2018.07.003.Peer-Reviewed Original ResearchLong-Term Outcomes of Free Gracilis Muscle Transfer for Smile Reanimation in Children
Greene J, Tavares J, Mohan S, Jowett N, Hadlock T. Long-Term Outcomes of Free Gracilis Muscle Transfer for Smile Reanimation in Children. The Journal Of Pediatrics 2018, 202: 279-284.e2. PMID: 30054167, DOI: 10.1016/j.jpeds.2018.06.043.Peer-Reviewed Original ResearchConceptsFree gracilis muscle transferFacial palsyGracilis muscle transferSmile excursionQuality of lifeMuscle transferPostoperative photographySmile reanimationFacial Clinimetric Evaluation (FaCE) surveyPatient-based instrumentLong-term outcomesCohort of childrenFacial nerve centerFacial symmetryPediatric patientsPostoperative qualityLife scoresTerm outcomesRetrospective analysisMassachusetts EyePalsySignificant improvementFacial impairmentPatientsFacial asymmetryOpioids and the Otolaryngologist: An Ambulatory Assessment
Mohan S, Bhattacharyya N. Opioids and the Otolaryngologist: An Ambulatory Assessment. Otolaryngology 2018, 159: 29-34. PMID: 29557264, DOI: 10.1177/0194599818765125.Peer-Reviewed Original ResearchConceptsOpioid prescriptionsOtolaryngology visitsOpioid prescribingCalendar trendsNational Ambulatory Medical Care SurveyAmbulatory Medical Care SurveyDesign Cross-sectional analysisOpioid medication prescribingOpioid prescription patternsChronic otitis mediaOpioid prescription ratesAmbulatory care settingsCross-sectional analysisOutpatient opioidsNational survey databasePrescription patternsNasal obstructionPrescription ratesMedication prescribingPostoperative careOtitis externaOtitis mediaCare SurveyOpioid epidemicCare settingsAssessment of Disease‐Specific and General Patient‐Reported Outcome Measures of Hearing Health
Mohan S, Corrales C, Yueh B, Shin J. Assessment of Disease‐Specific and General Patient‐Reported Outcome Measures of Hearing Health. Otolaryngology 2018, 158: 702-709. PMID: 29460681, DOI: 10.1177/0194599818757998.Peer-Reviewed Original ResearchConceptsPROMIS scoresTertiary care academic medical centerMental health summary scoresGlobal itemMental healthPhysical healthInner earProspective cohort dataDisease-specific assessmentsGeneral health statusHealth summary scoresComposite scoreItem scoresAcademic medical centerMean scoreChief complaintPROMIS instrumentsDisease-SpecificGeneral patientsHearing lossOutcome measuresMedical CenterMethods AdultsSummary scoresHealth status
2017
Modified approach for pediatric external cricopharyngeal myotomy
Mohan S, Bowe S, Hirner L, Zar-Kessler C, Hartnick C. Modified approach for pediatric external cricopharyngeal myotomy. International Journal Of Pediatric Otorhinolaryngology 2017, 105: 111-114. PMID: 29447795, DOI: 10.1016/j.ijporl.2017.12.019.Peer-Reviewed Original ResearchConceptsOpen cricopharyngeal myotomyCricopharyngeal myotomyCricopharyngeal achalasiaUpper esophageal sphincter relaxationPediatric cricopharyngeal achalasiaBotulinum toxin injectionEsophageal sphincter relaxationRecurrent laryngeal nerveBalloon dilationEsophageal perforationSphincter relaxationToxin injectionLaryngeal nerveOropharyngeal dysphagiaPosterior midlineImportant causeRisky procedureYoung malesAchalasiaMyotomyDysphagiaCricopharyngeusNervePathophysiologyAdjuvant radiotherapy is not supported in patients with verrucous carcinoma of the oral cavity
Mohan S, Pai S, Bhattacharyya N. Adjuvant radiotherapy is not supported in patients with verrucous carcinoma of the oral cavity. The Laryngoscope 2017, 127: 1334-1338. PMID: 28150306, PMCID: PMC7388729, DOI: 10.1002/lary.26443.Peer-Reviewed Original ResearchMeSH KeywordsAgedCarcinoma, Squamous CellCarcinoma, VerrucousCross-Sectional StudiesDisease-Free SurvivalFemaleHead and Neck NeoplasmsHumansKaplan-Meier EstimateMaleMiddle AgedMouthMouth NeoplasmsNeoplasm Recurrence, LocalRadiotherapy, AdjuvantSEER ProgramSquamous Cell Carcinoma of Head and NeckTreatment OutcomeConceptsAdjuvant radiation therapyDisease-specific survivalSquamous cell carcinomaVerrucous carcinomaRadiation therapyOverall survivalOral cavityVC patientsWorse OSCarcinoma patientsImproved overall survivalEnd Results (SEER) databaseKaplan-Meier survivalAdjuvant radiotherapySCC patientsLocal recurrenceResults databaseT stagePositive marginsSCC casesCell carcinomaPatientsSurgeryCarcinomaDifferent biology
2016
SMAC Mimetic Birinapant plus Radiation Eradicates Human Head and Neck Cancers with Genomic Amplifications of Cell Death Genes FADD and BIRC2
Eytan D, Snow G, Carlson S, Derakhshan A, Saleh A, Schiltz S, Cheng H, Mohan S, Cornelius S, Coupar J, Sowers A, Hernandez L, Mitchell J, Annunziata C, Chen Z, Van Waes C. SMAC Mimetic Birinapant plus Radiation Eradicates Human Head and Neck Cancers with Genomic Amplifications of Cell Death Genes FADD and BIRC2. Cancer Research 2016, 76: 5442-5454. PMID: 27469115, PMCID: PMC5026594, DOI: 10.1158/0008-5472.can-15-3317.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntineoplastic AgentsBlotting, WesternCarcinoma, Squamous CellCell Line, TumorChemoradiotherapyDipeptidesFas-Associated Death Domain ProteinFemaleGene AmplificationGene Knockdown TechniquesHead and Neck NeoplasmsHumansImmunohistochemistryIndolesInhibitor of Apoptosis ProteinsMiceMice, Inbred BALB CMice, SCIDReal-Time Polymerase Chain ReactionSquamous Cell Carcinoma of Head and NeckTNF-Related Apoptosis-Inducing LigandTumor Necrosis Factor-alphaUbiquitin-Protein LigasesXenograft Model Antitumor AssaysConceptsSmac mimeticsCell deathNovel Smac mimeticsCaspase-dependent apoptosisExpression of FADDFrequent genomic amplificationCell cycle arrestBaculovirus inhibitorG2-M cell cycle arrestApoptosis repeatCancer Genome AtlasDeath domainIAP inhibitorsFunctional importanceSiRNA knockdownDNA fragmentationHNSCC cell linesFADDBirinapantGenomic amplificationGene transferGenome AtlasProtein markersComparison of tumorGenomic alterations
2015
MEK Inhibitor PD-0325901 Overcomes Resistance to PI3K/mTOR Inhibitor PF-5212384 and Potentiates Antitumor Effects in Human Head and Neck Squamous Cell Carcinoma
Mohan S, Vander Broek R, Shah S, Eytan D, Pierce M, Carlson S, Coupar J, Zhang J, Cheng H, Chen Z, Van Waes C. MEK Inhibitor PD-0325901 Overcomes Resistance to PI3K/mTOR Inhibitor PF-5212384 and Potentiates Antitumor Effects in Human Head and Neck Squamous Cell Carcinoma. Clinical Cancer Research 2015, 21: 3946-3956. PMID: 25977343, PMCID: PMC4558307, DOI: 10.1158/1078-0432.ccr-14-3377.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntineoplastic AgentsBenzamidesCarcinoma, Squamous CellCell Line, TumorCell ProliferationCell SurvivalCell Transformation, NeoplasticCytokinesDiphenylamineDisease Models, AnimalDrug Resistance, NeoplasmDrug SynergismGene ExpressionGenes, ReporterHead and Neck NeoplasmsHumansInflammation MediatorsMitogen-Activated Protein Kinase KinasesMorpholinesNF-kappa BPhosphatidylinositol 3-KinasesPhosphoinositide-3 Kinase InhibitorsPhosphorylationProtein Kinase InhibitorsSignal TransductionSquamous Cell Carcinoma of Head and NeckTOR Serine-Threonine KinasesTranscription Factor AP-1Transcriptional ActivationTriazinesXenograft Model Antitumor AssaysMEK Inhibitor PD-0325901 Overcomes Resistance to CK2 Inhibitor CX-4945 and Exhibits Anti-Tumor Activity in Head and Neck Cancer
Bian Y, Han J, Kannabiran V, Mohan S, Cheng H, Friedman J, Zhang L, VanWaes C, Chen Z. MEK Inhibitor PD-0325901 Overcomes Resistance to CK2 Inhibitor CX-4945 and Exhibits Anti-Tumor Activity in Head and Neck Cancer. International Journal Of Biological Sciences 2015, 11: 411-422. PMID: 25798061, PMCID: PMC4366640, DOI: 10.7150/ijbs.10745.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBenzamidesBlotting, WesternCasein Kinase IICell Line, TumorDiphenylamineFemaleFlow CytometryHead and Neck NeoplasmsHumansJNK Mitogen-Activated Protein KinasesMiceMice, Inbred BALB CMice, SCIDNaphthyridinesNF-kappa BPhenazinesProto-Oncogene Proteins c-fosTumor Suppressor Protein p53Xenograft Model Antitumor AssaysConceptsAnti-tumor activityMEK inhibitor PD-0325901PD-901CK2 inhibitor CXModest anti-tumor activityNeck squamous cell carcinomaPD-0325901Cell linesSignificant anti-tumor effectHuman HNSCC cell linesUM-SCC cell linesP-AKT T308Squamous cell carcinomaIL-8 activityFurther clinical investigationAnti-tumor effectsHNSCC cell linesDNA flow cytometryAP-1 pathwayDrug resistance mechanismsExhibit anti-tumor activityResistance mechanismsUM-SCC1Cell carcinomaNeck cancer
2014
A Case of Trigeminocardiac Reflex During Infrastructure Maxillectomy
Mohan S, Flis D, O'Leary M. A Case of Trigeminocardiac Reflex During Infrastructure Maxillectomy. JAMA Otolaryngology - Head & Neck Surgery 2014, 140: 563-564. PMID: 24788865, DOI: 10.1001/jamaoto.2014.562.Peer-Reviewed Original ResearchConceptsTrigeminocardiac reflexInfrastructure maxillectomyTertiary academic medical centerEpisodes of asystoleAdministration of atropineSquamous cell carcinomaAcademic medical centerArterial hypotensionSensory branchSkull base proceduresAnticholinergic useInciting stimulusCell carcinomaTrigeminal nerveTrigeminal branchesNeck surgeonsMedical CenterBetter outcomesCareful dissectionEarly interventionProcedure completionReflexMaxillectomySudden developmentBradycardia
2013
The PI3K/Akt/mTOR axis in head and neck cancer: functions, aberrations, cross‐talk, and therapies
Broek R, Mohan S, Eytan D, Chen Z, Van Waes C. The PI3K/Akt/mTOR axis in head and neck cancer: functions, aberrations, cross‐talk, and therapies. Oral Diseases 2013, 21: 815-825. PMID: 24219320, DOI: 10.1111/odi.12206.Peer-Reviewed Original ResearchConceptsAkt/mTOR axisPI3K/Akt/mTOR axisDNA copy number variationsMTOR axisCopy number variationsPI3K/Akt/mTORDysregulation of mRNAsNeck squamous cell carcinomaPI3K/AKT/mTOR pathwayAKT/mTOR pathwayAkt/mTORSquamous cell carcinomaPathway leadCell survivalNumber variationsPI3KMalignant phenotypeMTOR pathwayNormal physiologyCell carcinomaHNSCC casesNeck cancerClinical studiesProtein expressionGenetic aberrations