2008
Visual Analysis of the Yeast 5S rRNA Gene Transcriptome: Regulation and Role of La Protein
French SL, Osheim YN, Schneider DA, Sikes ML, Fernandez CF, Copela LA, Misra VA, Nomura M, Wolin SL, Beyer AL. Visual Analysis of the Yeast 5S rRNA Gene Transcriptome: Regulation and Role of La Protein. Molecular And Cellular Biology 2008, 28: 4576-4587. PMID: 18474615, PMCID: PMC2447126, DOI: 10.1128/mcb.00127-08.Peer-Reviewed Original ResearchConceptsActive genesLa proteinPolymerase loadingRNA polymerase III genesYeast La proteinGrowth conditionsGene copy numberTranscription terminationDifferent growth conditionsShort genesRNA polymeraseGene transcriptomeRRNA geneMiller chromatinGenesMore polymerasesCopy numberElongation rateLHP1First quantitative analysisPolymeraseProteinRegulationFourfold rangeTranscriptomeCompetition between the Rex1 exonuclease and the La protein affects both Trf4p-mediated RNA quality control and pre-tRNA maturation
Copela LA, Fernandez CF, Sherrer RL, Wolin SL. Competition between the Rex1 exonuclease and the La protein affects both Trf4p-mediated RNA quality control and pre-tRNA maturation. RNA 2008, 14: 1214-1227. PMID: 18456844, PMCID: PMC2390804, DOI: 10.1261/rna.1050408.Peer-Reviewed Original ResearchConceptsLa proteinQuality control pathwaysPre-tRNA maturationRNA quality controlWild-type cellsChoice of pathwaySRP RNAExonucleolytic trimmingAberrant RNAsDefective RNAsMutant yeastFunctional RNAsMajor exonucleaseNoncoding RNAsControl pathwaysRNAExonucleaseTrf4pProteinYeastPathwayCellsNascentAccumulationMaturation
2006
The La protein functions redundantly with tRNA modification enzymes to ensure tRNA structural stability
Copela LA, Chakshusmathi G, Sherrer RL, Wolin SL. The La protein functions redundantly with tRNA modification enzymes to ensure tRNA structural stability. RNA 2006, 12: 644-654. PMID: 16581807, PMCID: PMC1421099, DOI: 10.1261/rna.2307206.Peer-Reviewed Original ResearchConceptsModification enzymesArginyl-tRNA synthetaseFission yeastPseudouridine synthasesTRNA decayTRNA modificationsEfficient biogenesisFunctional redundancyProtein functionMutant tRNAsLa proteinTRNA synthetaseYeast cellsTRNABiogenesisMultiple pathwaysProteinPresence of mutationsMutationsEnzymePathwayPUS4Trf4pGrowthPUS3