2002
Exclusive Interaction of the 15.5 kD Protein with the Terminal Box C/D Motif of a Methylation Guide snoRNP
Szewczak LB, DeGregorio SJ, Strobel SA, Steitz JA. Exclusive Interaction of the 15.5 kD Protein with the Terminal Box C/D Motif of a Methylation Guide snoRNP. Cell Chemical Biology 2002, 9: 1095-1107. PMID: 12401494, DOI: 10.1016/s1074-5521(02)00239-9.Peer-Reviewed Original ResearchConceptsBox C/D motifKD proteinD motifBox C/D snoRNAsBox C/D snoRNPsSite-specific methylationSpliceosomal RNAsDeleterious substitutionsD snoRNAsD snoRNPsBox DExclusive interactionsInteraction sitesXenopus oocytesSnoRNPsProteinMotifSnoRNAsVivoMethylationA89Single interaction siteRNAExocyclic amineOocytesSite‐specific cross‐linking analyses reveal an asymmetric protein distribution for a box C/D snoRNP
Cahill NM, Friend K, Speckmann W, Li Z, Terns RM, Terns MP, Steitz JA. Site‐specific cross‐linking analyses reveal an asymmetric protein distribution for a box C/D snoRNP. The EMBO Journal 2002, 21: 3816-3828. PMID: 12110593, PMCID: PMC126121, DOI: 10.1093/emboj/cdf376.Peer-Reviewed Original ResearchConceptsBox C/D snoRNPsD snoRNPsC-boxBox C/D classSite-specific cross-linking analysisBox C/D small nucleolar ribonucleoproteinsBox C/DAsymmetric protein distributionSmall nucleolar ribonucleoproteinSmall nucleolar RNAsXenopus oocyte nucleusCross-linking analysisNucleolar RNAsNucleolar ribonucleoproteinD motifBox DMutational analysisOocyte nucleusGuide sequenceWidespread modificationProtein distributionFibrillarinFunctional rescueCore proteinSnoRNPs
1991
An intact Box C sequence in the U3 snRNA is required for binding of fibrillarin, the protein common to the major family of nucleolar snRNPs.
Baserga SJ, Yang XD, Steitz JA. An intact Box C sequence in the U3 snRNA is required for binding of fibrillarin, the protein common to the major family of nucleolar snRNPs. The EMBO Journal 1991, 10: 2645-51. PMID: 1714385, PMCID: PMC452965, DOI: 10.1002/j.1460-2075.1991.tb07807.x.Peer-Reviewed Original ResearchConceptsBox CU3 snRNANucleolar small RNAsSite-specific mutationsShort nucleotide sequencesFibrillarin proteinSmall RNAsDeletion analysisCommon binding siteBox DNucleotide sequenceSnRNPsMajor familiesSnRNAU3 snRNPRNAInput RNAFibrillarinBinding sitesC sequencesBindingProteinSequenceAnti-fibrillarin autoantibodiesBiogenesis