2022
Conserved meningeal lymphatic drainage circuits in mice and humans
Jacob L, de Brito Neto J, Lenck S, Corcy C, Benbelkacem F, Geraldo LH, Xu Y, Thomas JM, Kamouh M, Spajer M, Potier MC, Haik S, Kalamarides M, Stankoff B, Lehericy S, Eichmann A, Thomas JL. Conserved meningeal lymphatic drainage circuits in mice and humans. Journal Of Experimental Medicine 2022, 219: e20220035. PMID: 35776089, PMCID: PMC9253621, DOI: 10.1084/jem.20220035.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsGlymphatic SystemHumansLymphatic SystemLymphatic VesselsMagnetic Resonance ImagingMeningesMiceConceptsCerebrospinal fluidVessel wall magnetic resonance imagingDural venous sinusesMagnetic resonance imagingCavernous sinusSystemic injectionImmune surveillanceVenous sinusesGlymphatic systemNeurological diseasesDura materVW-MRIResonance imagingBrain tissueEmissary veinsNeurological pathologiesAnterior partCSF outflowTracer injectionDrainage circuitThree-dimensional anatomyLymphatic vesselsPatientsDiagnostic toolSinus
2020
Les vaisseaux lymphatiques méningés, une cible potentielle pour le traitement des tumeurs cérébrales
Thomas JL, Song E, Boisserand L, Iwasaki A. Les vaisseaux lymphatiques méningés, une cible potentielle pour le traitement des tumeurs cérébrales. Médecine/sciences 2020, 36: 709-713. PMID: 32821046, PMCID: PMC8158397, DOI: 10.1051/medsci/2020141.Peer-Reviewed Original ResearchThree-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy.
Jacob L, Brito J, Thomas JL. Three-Dimensional Imaging of the Vertebral Lymphatic Vasculature and Drainage using iDISCO+ and Light Sheet Fluorescence Microscopy. Journal Of Visualized Experiments 2020 PMID: 32510513, DOI: 10.3791/61099.Peer-Reviewed Original ResearchMeSH KeywordsCentral Nervous SystemHumansImaging, Three-DimensionalLightLymphatic VesselsMicroscopy, FluorescenceNeovascularization, PhysiologicSpineConceptsLight sheet fluorescence microscopySheet fluorescence microscopyLymphatic vasculatureFluorescence microscopyCentral nervous systemLymphatic vesselsBiologyLymphatic systemLymphatic networkJoint biologyWhole-mount preparationsUnprecedented opportunityImmune surveillanceNervous systemImmune cellsTissueMount preparationsVertebral columnCNS tissueCellsMagnaMacromoleculesVEGF-C-driven lymphatic drainage enables immunosurveillance of brain tumours
Song E, Mao T, Dong H, Boisserand LSB, Antila S, Bosenberg M, Alitalo K, Thomas JL, Iwasaki A. VEGF-C-driven lymphatic drainage enables immunosurveillance of brain tumours. Nature 2020, 577: 689-694. PMID: 31942068, PMCID: PMC7100608, DOI: 10.1038/s41586-019-1912-x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrain NeoplasmsCD8-Positive T-LymphocytesCell Cycle CheckpointsCell Line, TumorCell MovementCentral Nervous SystemCross-PrimingFemaleGlioblastomaHEK293 CellsHumansImmunologic MemoryImmunologic SurveillanceLymph NodesLymphangiogenesisLymphatic VesselsMaleMelanomaMeningesMiceMice, Inbred C57BLProgrammed Cell Death 1 ReceptorVascular Endothelial Growth Factor CConceptsCD8 T cellsCentral nervous systemT cellsImmune responseBrain tumorsImmune surveillanceLymphatic drainageNervous systemAntigen-specific immune responsesDeep cervical lymph nodesCapacity of VEGFCervical lymph nodesCheckpoint blockade therapyMeningeal lymphatic systemVascular endothelial growth factor CNew therapeutic approachesUncontrolled tumor growthMeningeal lymphatic vasculatureBlockade therapyLymph nodesTherapeutic approachesMouse modelTumor growthMemory responsesTumors
2019
Anatomy and function of the vertebral column lymphatic network in mice
Jacob L, Boisserand LSB, Geraldo LHM, de Brito Neto J, Mathivet T, Antila S, Barka B, Xu Y, Thomas JM, Pestel J, Aigrot MS, Song E, Nurmi H, Lee S, Alitalo K, Renier N, Eichmann A, Thomas JL. Anatomy and function of the vertebral column lymphatic network in mice. Nature Communications 2019, 10: 4594. PMID: 31597914, PMCID: PMC6785564, DOI: 10.1038/s41467-019-12568-w.Peer-Reviewed Original ResearchConceptsLymphatic vesselsCentral nervous system immune responseFocal spinal cord lesionsT cell infiltrationSpinal cord lesionsSpinal cord injuryCNS immunityCord lesionsMeningeal lymphatic vesselsSympathetic gangliaCord injuryCell infiltrationSpinal cordInflammatory responseEpidural spaceThoracic ductImmune responseDura materSpinal tissuePotential targetVertebral tissuesLymphatic networkSpine segmentsTraditional histologyLittle informationLymphatic system in central nervous system
Thomas JL, Jacob L, Boisserand L. Lymphatic system in central nervous system. Médecine/sciences 2019, 35: 55-61. PMID: 30672459, DOI: 10.1051/medsci/2018309.Peer-Reviewed Original ResearchConceptsCentral nervous systemCerebrospinal fluidImmune surveyNervous systemLymphatic vesselsLymphatic systemBrain interstitial fluidHuman CNS diseasesNew therapeutic targetsPeripheral lymphatic systemInterstitial fluid drainageCerebral drainageMeningeal lymphatic vasculatureInterstitial fluidCNS diseaseFluid drainageTherapeutic targetPerivascular spacesTissue drainageConsiderable metabolic activityVasculatureDrainageVesselsMetabolic activityLymphatic vasculature
2017
Development and plasticity of meningeal lymphatic vessels
Antila S, Karaman S, Nurmi H, Airavaara M, Voutilainen MH, Mathivet T, Chilov D, Li Z, Koppinen T, Park JH, Fang S, Aspelund A, Saarma M, Eichmann A, Thomas JL, Alitalo K. Development and plasticity of meningeal lymphatic vessels. Journal Of Experimental Medicine 2017, 214: 3645-3667. PMID: 29141865, PMCID: PMC5716035, DOI: 10.1084/jem.20170391.Peer-Reviewed Original ResearchAnimalsAnimals, NewbornBiological TransportCerebrospinal FluidDependovirusGene DeletionHumansIndolesInjections, IntraventricularLymph NodesLymphangiogenesisLymphatic VesselsMaleMeningesMice, Inbred C57BLMicrospheresMyocytes, Smooth MuscleProtein Kinase InhibitorsPyrrolesSignal TransductionSpinal CordSunitinibVascular Endothelial Growth Factor CVascular Endothelial Growth Factor DVascular Endothelial Growth Factor Receptor-3
2012
Semaphorin3A, Neuropilin-1, and PlexinA1 Are Required for Lymphatic Valve Formation
Bouvrée K, Brunet I, del Toro R, Gordon E, Prahst C, Cristofaro B, Mathivet T, Xu Y, Soueid J, Fortuna V, Miura N, Aigrot MS, Maden CH, Ruhrberg C, Thomas JL, Eichmann A. Semaphorin3A, Neuropilin-1, and PlexinA1 Are Required for Lymphatic Valve Formation. Circulation Research 2012, 111: 437-445. PMID: 22723296, PMCID: PMC3861899, DOI: 10.1161/circresaha.112.269316.Peer-Reviewed Original ResearchAnimalsAnimals, NewbornAntibodies, NeutralizingBacterial ProteinsCells, CulturedEndothelial CellsGene Expression Regulation, DevelopmentalGenotypeGestational AgeHumansLuminescent ProteinsLymphatic VesselsMiceMice, KnockoutMice, TransgenicMorphogenesisNerve Tissue ProteinsNeuropilin-1PhenotypeReceptors, Cell SurfaceRNA, MessengerSemaphorin-3AVascular Endothelial Growth Factor Receptor-3
2010
Neuropilin-2 mediates VEGF-C–induced lymphatic sprouting together with VEGFR3
Xu Y, Yuan L, Mak J, Pardanaud L, Caunt M, Kasman I, Larrivée B, del Toro R, Suchting S, Medvinsky A, Silva J, Yang J, Thomas JL, Koch AW, Alitalo K, Eichmann A, Bagri A. Neuropilin-2 mediates VEGF-C–induced lymphatic sprouting together with VEGFR3. Journal Of Cell Biology 2010, 188: 115-130. PMID: 20065093, PMCID: PMC2812843, DOI: 10.1083/jcb.200903137.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell ShapeCells, CulturedEndothelial CellsFemaleLymphangiogenesisLymphatic VesselsMaleMiceMice, Inbred C57BLMice, Inbred StrainsMice, TransgenicNeuropilin-2Protein BindingVascular Endothelial Growth Factor CVascular Endothelial Growth Factor Receptor-2Vascular Endothelial Growth Factor Receptor-3ConceptsLymphatic vessel sproutingVEGF receptor 2Lymphangiogenic vascular endothelial growth factors CSprouting defectsNeuropilin-2Vessel sproutingVascular endothelial growth factor CVEGF-C bindingAntibody treatmentEndothelial tip cellsReceptor 2Lymph vesselsLymphatic sproutingGenetic deletionHeterozygous miceTransmembrane receptorsTip cellsAdult organsMiceCell extensionsNRP2Vascular systemVascular sprout formationVascular sproutingVEGF