2011
GEP constitutes a negative feedback loop with MyoD and acts as a novel mediator in controlling skeletal muscle differentiation
Wang D, Bai X, Tian Q, Lai Y, Lin E, Shi Y, Mu X, Feng J, Carlson C, Liu C. GEP constitutes a negative feedback loop with MyoD and acts as a novel mediator in controlling skeletal muscle differentiation. Cellular And Molecular Life Sciences 2011, 69: 1855-1873. PMID: 22179841, PMCID: PMC3319484, DOI: 10.1007/s00018-011-0901-5.Peer-Reviewed Original ResearchConceptsMyoD transcription factorGranulin-epithelin precursorTranscription factorsMyoblast fusionMyotube formationMuscle-specific transcription factorsC2C12 myoblast fusionE-box sequenceSkeletal muscle differentiationFusion of myoblastsNegative feedback loopGrowth factorEmbryonic developmentRegulatory regionsMuscle differentiationSkeletal muscle tissueC2C12 myoblastsRegulatory loopSiRNA approachNovel mediatorAutocrine growth factorJunBKnockdown miceMyoblastsTissue repair
2010
Granulin epithelin precursor: a bone morphogenic protein 2‐inducible growth factor that activates Erk1/2 signaling and JunB transcription factor in chondrogenesis
Feng J, Guo F, Jiang B, Zhang Y, Frenkel S, Wang D, Tang W, Xie Y, Liu C. Granulin epithelin precursor: a bone morphogenic protein 2‐inducible growth factor that activates Erk1/2 signaling and JunB transcription factor in chondrogenesis. The FASEB Journal 2010, 24: 1879-1892. PMID: 20124436, PMCID: PMC2874481, DOI: 10.1096/fj.09-144659.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiomarkersBlotting, WesternBone Morphogenetic Protein 2CartilageCell DifferentiationCells, CulturedChondrocytesChondrogenesisChromatin ImmunoprecipitationFetusGene Expression ProfilingHumansImmunoenzyme TechniquesIn Situ HybridizationIntercellular Signaling Peptides and ProteinsMesenchymal Stem CellsMiceMice, Inbred C3HMice, KnockoutMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Oligonucleotide Array Sequence AnalysisProgranulinsProto-Oncogene Proteins c-junRabbitsReverse Transcriptase Polymerase Chain ReactionRNA ProbesRNA, MessengerSignal TransductionConceptsJunB transcription factorKey downstream moleculeGranulin-epithelin precursorChondrocyte differentiationTranscription factorsDownstream moleculesNovel critical roleGrowth factorBone morphogenic proteinBone morphogenic protein-2Molecular eventsMesenchymal stem cellsERK1/2 signalingProtein 2Morphogenic proteinStem cellsDifferentiationBMP2Critical roleTissue regenerationVivoSignalingCartilage repairProteinChondrogenesis
2009
Cartilage Oligomeric Matrix Protein Maintains the Contractile Phenotype of Vascular Smooth Muscle Cells by Interacting With &agr;7&bgr;1 Integrin
Wang L, Zheng J, Du Y, Huang Y, Li J, Liu B, Liu C, Zhu Y, Gao Y, Xu Q, Kong W, Wang X. Cartilage Oligomeric Matrix Protein Maintains the Contractile Phenotype of Vascular Smooth Muscle Cells by Interacting With &agr;7&bgr;1 Integrin. Circulation Research 2009, 106: 514-525. PMID: 20019333, DOI: 10.1161/circresaha.109.202762.Peer-Reviewed Original ResearchMeSH KeywordsAdenoviridaeAnimalsAntigens, CDAorta, ThoracicBecaplerminCarotid Artery InjuriesCatheterizationCell DifferentiationCells, CulturedExtracellular MatrixExtracellular Matrix ProteinsGene Knockdown TechniquesGenetic VectorsGlycoproteinsIntegrin alpha ChainsIntegrinsMaleMatrilin ProteinsMuscle ContractionMuscle ProteinsMuscle, Smooth, VascularMyocytes, Smooth MusclePhenotypePlatelet-Derived Growth FactorProtein Interaction MappingProto-Oncogene Proteins c-sisRatsRats, Sprague-DawleyRecombinant Fusion ProteinsRNA, Small InterferingConceptsVascular smooth muscle cellsCartilage oligomeric matrix proteinEffects of COMPContractile phenotypeFocal adhesion assemblyVSMC differentiation marker genesAdenoviral overexpressionSmooth muscle cellsNormal vascular smooth muscle cellsVSMC phenotype switchingDifferentiation marker genesActin fiber organizationMuscle cellsAdhesion assemblyVascular extracellular matrixVSMC contractile phenotypeMarker genesDifferentiated stateRat vascular smooth muscle cellsPhenotype switchingPlatelet-derived growthVSMC adhesionMatrix proteinsVSMC dedifferentiationSmall interferingThe p200 family protein p204 as a modulator of cell proliferation and differentiation: a brief survey
Lengyel P, Liu CJ. The p200 family protein p204 as a modulator of cell proliferation and differentiation: a brief survey. Cellular And Molecular Life Sciences 2009, 67: 335-340. PMID: 19921484, PMCID: PMC2824682, DOI: 10.1007/s00018-009-0195-z.Peer-Reviewed Original ResearchConceptsTranscription factorsTissue-specific transcription factorsParticular transcription factorsRibosomal RNA synthesisCell proliferationSkeletal muscle myotubesUnexplored tissuesMuscle myotubesCell cyclingDifferentiation proteinRNA synthesisNumerous tissuesP204DifferentiationProteinCardiac myocytesProliferationTissueMyotubesCytoplasmNucleoliOsteoblastsExpressionActivityRAADAMTS-7, a Direct Target of PTHrP, Adversely Regulates Endochondral Bone Growth by Associating with and Inactivating GEP Growth Factor
Bai X, Wang D, Kong L, Zhang Y, Luan Y, Kobayashi T, Kronenberg H, Yu X, Liu C. ADAMTS-7, a Direct Target of PTHrP, Adversely Regulates Endochondral Bone Growth by Associating with and Inactivating GEP Growth Factor. Molecular And Cellular Biology 2009, 29: 4201-4219. PMID: 19487464, PMCID: PMC2715794, DOI: 10.1128/mcb.00056-09.Peer-Reviewed Original ResearchMeSH KeywordsADAM ProteinsADAMTS7 ProteinAnimalsBlotting, WesternBone and BonesBone DevelopmentCell DifferentiationCell LineCell Line, TumorChondrocytesGene Expression ProfilingHumansImmunohistochemistryImmunoprecipitationIntercellular Signaling Peptides and ProteinsMiceMice, KnockoutParathyroid Hormone-Related ProteinProgranulinsProtein BindingProtein PrecursorsReverse Transcriptase Polymerase Chain ReactionTissue Culture TechniquesTwo-Hybrid System TechniquesConceptsADAMTS-7Granulin-epithelin precursorEndochondral bone formationEndochondral bone growthGrowth factorBone formationChondrocyte differentiationBone growthDirect targetAutocrine growth factorGrowth plate chondrocytesProteolytic activityPTHrPChondrogenic growth factorsChondrocyte hypertrophyExtracellular matrix proteinsCartilage extracellular matrix proteinsImportant targetADAMTS familyInhibitionSkeletal developmentMatrix proteinsExpression patternsExtracellular matrixArthritismiR-199a*, a Bone Morphogenic Protein 2-responsive MicroRNA, Regulates Chondrogenesis via Direct Targeting to Smad1*
Lin E, Kong L, Bai X, Luan Y, Liu C. miR-199a*, a Bone Morphogenic Protein 2-responsive MicroRNA, Regulates Chondrogenesis via Direct Targeting to Smad1*. Journal Of Biological Chemistry 2009, 284: 11326-11335. PMID: 19251704, PMCID: PMC2670138, DOI: 10.1074/jbc.m807709200.Peer-Reviewed Original Research
2008
Regulation of chondrocyte differentiation by ADAMTS-12 metalloproteinase depends on its enzymatic activity
Bai X, Wang D, Luan Y, Yu X, Liu C. Regulation of chondrocyte differentiation by ADAMTS-12 metalloproteinase depends on its enzymatic activity. Cellular And Molecular Life Sciences 2008, 66: 667. PMID: 19151918, PMCID: PMC11131527, DOI: 10.1007/s00018-008-8633-x.Peer-Reviewed Original ResearchConceptsADAMTS-12Chondrocyte differentiationGrowth plate chondrocytesType X collagen expressionThrombospondin motifsPTHrPCollagen expressionAltered expressionMRNA levelsProminent expressionDownstream moleculesADAMTS familyMetalloproteinaseInhibitionEnzymatic activityNovel regulatorProteolytic activityChondrocytesExpressionChondrogenesisDifferentiationActivityp204, a p200 family protein, as a multifunctional regulator of cell proliferation and differentiation
Luan Y, Lengyel P, Liu CJ. p204, a p200 family protein, as a multifunctional regulator of cell proliferation and differentiation. Cytokine & Growth Factor Reviews 2008, 19: 357-369. PMID: 19027346, PMCID: PMC4442486, DOI: 10.1016/j.cytogfr.2008.11.002.Peer-Reviewed Original ResearchConceptsP200-family proteinsFamily proteinsAmino acid-long C-terminal domainLong C-terminal domainTissue-specific transcription factorsCell proliferationInterferon-inducible p200 familyTissue differentiationC-terminal domainMurine memberRas proteinsSubcellular compartmentsTranscription factorsHuman proteinsMultifunctional regulatorHomologous mouseTarget proteinsProteinTarget activityDifferentiationP204ProliferationTumor growthImportant roleFamilyMediation of Chondrogenic and Osteogenic Differentiation by an Interferon-Inducible p202 Protein
Kong L, Liu C. Mediation of Chondrogenic and Osteogenic Differentiation by an Interferon-Inducible p202 Protein. Cellular And Molecular Life Sciences 2008, 65: 3494-3506. PMID: 18791844, PMCID: PMC11131663, DOI: 10.1007/s00018-008-8342-5.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCell LineChondrocytesChondrogenesisFeedback, PhysiologicalGene Expression Regulation, DevelopmentalGene Knockdown TechniquesGenes, ReporterGrowth PlateIntracellular Signaling Peptides and ProteinsMiceMice, Inbred C3HMice, KnockoutMice, TransgenicOsteoblastsOsteogenesisParathyroid Hormone-Related ProteinPluripotent Stem CellsRNA, Small InterferingSmad ProteinsConceptsParathyroid hormone-related peptideExpression of PTHrPHormone-related peptideCourse of osteogenesisGrowth plate chondrocytesInterferon-inducible proteinMolecular mechanism studiesInterferon-inducible p200 familyImportant mediatorP202 proteinOsteogenic differentiationSiRNA approachMouse embryosP202 expressionChondrocyte differentiationPositive feedback loopSmad transcription factorsTransgenic mouse embryosOsteoblast differentiationDifferential expressionExpressionC3H10T1/2 cellsC2C12 cellsDifferentiationCellsCbfa1-dependent expression of an interferon-inducible p204 protein is required for chondrocyte differentiation
Zhang Y, Kong L, Carlson C, Liu C. Cbfa1-dependent expression of an interferon-inducible p204 protein is required for chondrocyte differentiation. Cell Death & Differentiation 2008, 15: 1760-1771. PMID: 18636074, DOI: 10.1038/cdd.2008.112.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBone Morphogenetic Protein 2Cell DifferentiationChondrocytesChondrogenesisCollagen Type XCore Binding Factor Alpha 1 SubunitEmbryo, MammalianGrowth PlateHedgehog ProteinsHypertrophyImmunohistochemistryMiceModels, BiologicalMolecular Sequence DataNuclear ProteinsParathyroid Hormone-Related ProteinPhosphoproteinsPromoter Regions, GeneticProtein BindingRNA, Small InterferingSignal TransductionSOXD Transcription FactorsTranscriptional ActivationConceptsChondrocyte hypertrophyChondrocyte differentiationMatrix metalloproteinase-13Indian hedgehogHypertrophic chondrocyte differentiationGrowth plate chondrocytesMetalloproteinase-13P204 proteinReceptor 1HypertrophyAltered levelsType X collagenAltered expressionEnhanced expressionProminent expressionSiRNA approachOverexpression of p204Collagen XSpecific reporter genesPluripotent C3H10T1/2 cellsX collagenNovel regulatorExpressionCbfa1C3H10T1/2 cellsp204 Protein Overcomes the Inhibition of Core Binding Factor α-1–mediated Osteogenic Differentiation by Id Helix-Loop-Helix Proteins
Luan Y, Yu X, Yang N, Frenkel S, Chen L, Liu C. p204 Protein Overcomes the Inhibition of Core Binding Factor α-1–mediated Osteogenic Differentiation by Id Helix-Loop-Helix Proteins. Molecular Biology Of The Cell 2008, 19: 2113-2126. PMID: 18287524, PMCID: PMC2366862, DOI: 10.1091/mbc.e07-10-1057.Peer-Reviewed Original ResearchMeSH KeywordsAlkaline PhosphataseAmino Acid SequenceAnimalsBone Morphogenetic Protein 2Bone Morphogenetic ProteinsCell DifferentiationCell LineCell NucleusCore Binding Factor alpha SubunitsFemaleHelix-Loop-Helix MotifsHumansInhibitor of Differentiation ProteinsMiceMice, Inbred BALB CNuclear Export SignalsNuclear ProteinsOsteoblastsOsteocalcinOsteogenesisPhosphoproteinsPromoter Regions, GeneticProteasome Endopeptidase ComplexProtein BindingProtein TransportTransforming Growth Factor betaUbiquitinConceptsD proteinsOsteogenic differentiationSequence-specific bindingUbiquitin-proteasome pathwayCore binding factor αExpression of Cbfa1Factor alpha 1P204 proteinExport signalHelix proteinsHelix-LoopRegulatory circuitsTarget genesInterferon-inducible proteinOsteocalcin geneMolecular mechanismsALP activityOsteoblast differentiationDiminished transcriptionCytoplasmic translocationId2Cbfa1Differentiation proteinProteinAlkaline phosphatase
2007
The Retinoblastoma Protein Is an Essential Mediator of Osteogenesis That Links the p204 Protein to the Cbfa1 Transcription Factor Thereby Increasing Its Activity*
Luan Y, Yu XP, Xu K, Ding B, Yu J, Huang Y, Yang N, Lengyel P, Di Cesare PE, Liu CJ. The Retinoblastoma Protein Is an Essential Mediator of Osteogenesis That Links the p204 Protein to the Cbfa1 Transcription Factor Thereby Increasing Its Activity*. Journal Of Biological Chemistry 2007, 282: 16860-16870. PMID: 17439944, DOI: 10.1074/jbc.m610943200.Peer-Reviewed Original ResearchConceptsGene activationTranscription factorsRetinoblastoma proteinProtein-protein interactionsChromatin immunoprecipitation assaysMesenchymal cell lineSkeletal muscle myotubesP204 expressionP204 proteinCore-binding factor alpha1Numerous proteinsImmunoprecipitation assaysSuch mutantsOsteocalcin geneReporter geneGene expressionAntisense RNAMuscle myotubesOsteoblast differentiationCbfa1Factor alpha1ProteinEssential mediatorTernary complexCell lines