Pan-pathogen deep sequencing of nosocomial bacterial pathogens in Italy in spring 2020: a prospective cohort study
Thorpe H, Pesonen M, Corbella M, Pesonen H, Gaiarsa S, Boinett C, Tonkin-Hill G, Mäklin T, Pöntinen A, MacAlasdair N, Gladstone R, Arredondo-Alonso S, Kallonen T, Jamrozy D, Lo S, Chaguza C, Blackwell G, Honkela A, Schürch A, Willems R, Merla C, Petazzoni G, Feil E, Cambieri P, Thomson N, Bentley S, Sassera D, Corander J. Pan-pathogen deep sequencing of nosocomial bacterial pathogens in Italy in spring 2020: a prospective cohort study. The Lancet Microbe 2024, 100890. PMID: 39178869, DOI: 10.1016/s2666-5247(24)00113-7.Peer-Reviewed Original ResearchIntensive care unitProspective cohort studyCohort studyHospital transmissionWhole-genome sequencingCare unitDeep sequencing approachSingle nucleotide polymorphism distancesNosocomial bacterial pathogensInfection control measuresAntimicrobial resistance genesPathogenic speciesBacterial pathogensDeep sequencingEffectiveness of infection control measuresAntimicrobial resistance genes prevalenceAcinetobacter baumanniiNosocomial infectionsStreptococcus pneumoniaeSan Matteo HospitalClinical surveillanceWithin-patientKlebsiella pneumoniaeCo-colonisationGene prevalenceMolecular Epidemiology of Isoniazid-resistant M tuberculosis in Port-au-Prince, Haiti
Walsh K, Lee M, Chaguza C, Pamphile W, Royal G, Escuyer V, Pape J, Fitzgerald D, Cohen T, Ocheretina O. Molecular Epidemiology of Isoniazid-resistant M tuberculosis in Port-au-Prince, Haiti. Open Forum Infectious Diseases 2024, 11: ofae421. PMID: 39119477, PMCID: PMC11306977, DOI: 10.1093/ofid/ofae421.Peer-Reviewed Original ResearchCulture-positive pulmonary tuberculosisIsoniazid-resistant isolatesHR-TBIsoniazid-resistant M. tuberculosisPulmonary tuberculosisIsoniazid resistanceMutations associated with INH resistanceTransmission clustersGenoType MTBDRplus assayINH-resistant isolatesIsoniazid-susceptible isolatesPhenotypic isoniazid resistanceRifampin-susceptible tuberculosisSingle nucleotide polymorphism distancesMolecular epidemiologyAssociated with poor treatment outcomesWhole-genome sequencingM. tuberculosisPutative transmission clustersPoor treatment outcomesMTBDRplus assayGenoType MTBDRplusCulture-based testsINH resistanceTuberculosis clinic