2023
Molecular programs of regional specification and neural stem cell fate progression in macaque telencephalon
Micali N, Ma S, Li M, Kim S, Mato-Blanco X, Sindhu S, Arellano J, Gao T, Shibata M, Gobeske K, Duque A, Santpere G, Sestan N, Rakic P. Molecular programs of regional specification and neural stem cell fate progression in macaque telencephalon. Science 2023, 382: eadf3786. PMID: 37824652, PMCID: PMC10705812, DOI: 10.1126/science.adf3786.Peer-Reviewed Original Research
2021
Radial Glial Cells: New Views on Old Questions
Arellano JI, Morozov YM, Micali N, Rakic P. Radial Glial Cells: New Views on Old Questions. Neurochemical Research 2021, 46: 2512-2524. PMID: 33725233, PMCID: PMC8855517, DOI: 10.1007/s11064-021-03296-z.Peer-Reviewed Original ResearchConceptsGlial fibrillary acidic proteinRadial glial cellsNeuroepithelial cellsGFAP expressionFibrillary acidic proteinMigration of neuronsProcess of neurogenesisGlial featuresGlial cellsEmbryonic cerebrumCortical neurogenesisMacaque monkeysPial surfaceAcidic proteinEpithelial featuresBrain developmentNeurogenesisVentricular surfaceTight junctionsCerebrumNeuronsUltrastructural analysisFirst descriptionBrainVertebrate brain
2020
Dissecting transcriptomic signatures of neuronal differentiation and maturation using iPSCs
Burke EE, Chenoweth JG, Shin JH, Collado-Torres L, Kim SK, Micali N, Wang Y, Colantuoni C, Straub RE, Hoeppner DJ, Chen HY, Sellers A, Shibbani K, Hamersky GR, Diaz Bustamante M, Phan BN, Ulrich WS, Valencia C, Jaishankar A, Price AJ, Rajpurohit A, Semick SA, Bürli RW, Barrow JC, Hiler DJ, Page SC, Martinowich K, Hyde TM, Kleinman JE, Berman KF, Apud JA, Cross AJ, Brandon NJ, Weinberger DR, Maher BJ, McKay RDG, Jaffe AE. Dissecting transcriptomic signatures of neuronal differentiation and maturation using iPSCs. Nature Communications 2020, 11: 462. PMID: 31974374, PMCID: PMC6978526, DOI: 10.1038/s41467-019-14266-z.Peer-Reviewed Original ResearchConceptsHuman induced pluripotent stem cellsNeural precursor cellsExpression dataSingle-cell expression dataNeuronal differentiationSequencing read alignmentsInduced pluripotent stem cellsEarly neuronal differentiationPluripotent stem cellsTranscriptomic resourcesIPSC donorNeuronal culturesSubclonal linesNeural differentiationTranscriptomic signaturesHuman neural precursor cellsNeuronal cellsStem cellsPrecursor cellsCell sortingGlobal patternsPowerful modelSubset of neuronsRead alignmentDifferentiation
2019
Gliogenesis in the outer subventricular zone promotes enlargement and gyrification of the primate cerebrum
Rash BG, Duque A, Morozov YM, Arellano JI, Micali N, Rakic P. Gliogenesis in the outer subventricular zone promotes enlargement and gyrification of the primate cerebrum. Proceedings Of The National Academy Of Sciences Of The United States Of America 2019, 116: 7089-7094. PMID: 30894491, PMCID: PMC6452694, DOI: 10.1073/pnas.1822169116.Peer-Reviewed Original ResearchConceptsOuter subventricular zoneSubventricular zoneWhite matterCompletion of neurogenesisSuperficial layers IISubcortical white matterUnderlying white matterProduction of astrocytesRadial glial cellsElaboration of dendritesCortical surface areaSuperficial neuronsCerebral neuronsCortical connectionsGlial cellsCerebral convolutionsDay 90Layers IIFormation of convolutionsMacaque monkeysRapid enlargementCerebrumNeuronal growthNeuronsEmbryonic day 90
2018
Metabolic regulation and glucose sensitivity of cortical radial glial cells
Rash BG, Micali N, Huttner AJ, Morozov YM, Horvath TL, Rakic P. Metabolic regulation and glucose sensitivity of cortical radial glial cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 2018, 115: 10142-10147. PMID: 30224493, PMCID: PMC6176632, DOI: 10.1073/pnas.1808066115.Peer-Reviewed Original ResearchConceptsRadial glial cellsGlial cellsRGC fibersCortical radial glial cellsEmbryonic cortical slicesGestational obesityCerebral cortexCortical slicesMetabolic disturbancesCortical neurogenesisMetabolic supportBrain disordersAcute lossMitochondrial transportBrain developmentIntracellular CaPotential mechanismsHyperglycemiaMitochondrial functionGlucose sensitivityMiceStem cellsPrimary stem cellsPhysiological mechanismsCells
2014
FGF2 and Insulin Signaling Converge to Regulate Cyclin D Expression in Multipotent Neural Stem Cells
Adepoju A, Micali N, Ogawa K, Hoeppner DJ, McKay RD. FGF2 and Insulin Signaling Converge to Regulate Cyclin D Expression in Multipotent Neural Stem Cells. Stem Cells 2014, 32: 770-778. PMID: 24155149, DOI: 10.1002/stem.1575.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell ProliferationCyclin DDNAFemaleFibroblast Growth Factor 2InsulinIntracellular SpaceMiceMice, Inbred C57BLModels, BiologicalMultipotent Stem CellsNeural Stem CellsProtein BiosynthesisProto-Oncogene Proteins c-fosProto-Oncogene Proteins c-junSignal TransductionTranscription, GeneticConceptsNeural stem cellsMultipotent neural stem cellsStem cellsPost-transcriptional levelPI3K/Akt pathwayTyrosine kinase receptorsPhosphorylation of ERK1/2Cell statesInsulin signalProliferation controlCell lineagesFibroblast growth factorBasic fibroblast growth factorCyclin D expressionMultipotent cellsC-JunD mRNA levelsKinase receptorsNeural precursorsAkt pathwayCell expansionMultipotent natureCentral nervous systemDisease mechanismsC-fos