2021
Ucp2-dependent microglia-neuronal coupling controls ventral hippocampal circuit function and anxiety-like behavior
Yasumoto Y, Stoiljkovic M, Kim JD, Sestan-Pesa M, Gao XB, Diano S, Horvath TL. Ucp2-dependent microglia-neuronal coupling controls ventral hippocampal circuit function and anxiety-like behavior. Molecular Psychiatry 2021, 26: 2740-2752. PMID: 33879866, PMCID: PMC8056795, DOI: 10.1038/s41380-021-01105-1.Peer-Reviewed Original ResearchConceptsAnxiety-like behaviorReactive oxygen speciesMicroglia-synapse contactsSpine synapse numberHippocampal circuit functionNeuronal circuit dysfunctionMicroglial productionVentral hippocampusCircuit dysfunctionSpine synapsesSynapse numberAdult brainTransient riseMitochondrial ROS generationMicrogliaBrain functionConditional ablationPhagocytic inclusionsSynaptic elementsProtein 2ROS generationSignificant reductionCircuit functionConsequent accumulationOxygen speciesDiscovery and functional interrogation of SARS-CoV-2 RNA-host protein interactions
Flynn RA, Belk JA, Qi Y, Yasumoto Y, Wei J, Alfajaro MM, Shi Q, Mumbach MR, Limaye A, DeWeirdt PC, Schmitz CO, Parker KR, Woo E, Chang HY, Horvath TL, Carette JE, Bertozzi CR, Wilen CB, Satpathy AT. Discovery and functional interrogation of SARS-CoV-2 RNA-host protein interactions. Cell 2021, 184: 2394-2411.e16. PMID: 33743211, PMCID: PMC7951565, DOI: 10.1016/j.cell.2021.03.012.Peer-Reviewed Original ResearchConceptsSARS-CoV-2 RNASARS-CoV-2Virus-induced cell deathHost protein interactionsRNA-binding proteinActive infectionRNA virusesHost-virus interfaceGlobal mortalityTherapeutic benefitCRISPR screensAntiviral factorsProtein interactionsAntiviral activityViral specificityHost pathwaysFunctional RNA-binding proteinsFunctional connectionsRNA-centric approachesCell deathHost proteinsVirusFunctional interrogationRNAComprehensive catalogNeuroinvasion of SARS-CoV-2 in human and mouse brain
Song E, Zhang C, Israelow B, Lu-Culligan A, Prado AV, Skriabine S, Lu P, Weizman OE, Liu F, Dai Y, Szigeti-Buck K, Yasumoto Y, Wang G, Castaldi C, Heltke J, Ng E, Wheeler J, Alfajaro MM, Levavasseur E, Fontes B, Ravindra NG, Van Dijk D, Mane S, Gunel M, Ring A, Kazmi SAJ, Zhang K, Wilen CB, Horvath TL, Plu I, Haik S, Thomas JL, Louvi A, Farhadian SF, Huttner A, Seilhean D, Renier N, Bilguvar K, Iwasaki A. Neuroinvasion of SARS-CoV-2 in human and mouse brain. Journal Of Experimental Medicine 2021, 218: e20202135. PMID: 33433624, PMCID: PMC7808299, DOI: 10.1084/jem.20202135.Peer-Reviewed Original ResearchConceptsSARS-CoV-2Central nervous systemSARS-CoV-2 neuroinvasionImmune cell infiltratesCOVID-19 patientsType I interferon responseMultiple organ systemsCOVID-19I interferon responseHuman brain organoidsNeuroinvasive capacityCNS infectionsCell infiltrateNeuronal infectionPathological featuresCortical neuronsRespiratory diseaseDirect infectionCerebrospinal fluidNervous systemMouse brainInterferon responseOrgan systemsHuman ACE2Infection
2020
SARS-CoV-2 infection of the placenta
Hosier H, Farhadian SF, Morotti RA, Deshmukh U, Lu-Culligan A, Campbell KH, Yasumoto Y, Vogels C, Casanovas-Massana A, Vijayakumar P, Geng B, Odio CD, Fournier J, Brito AF, Fauver JR, Liu F, Alpert T, Tal R, Szigeti-Buck K, Perincheri S, Larsen C, Gariepy AM, Aguilar G, Fardelmann KL, Harigopal M, Taylor HS, Pettker CM, Wyllie AL, Dela Cruz CS, Ring AM, Grubaugh ND, Ko AI, Horvath TL, Iwasaki A, Reddy UM, Lipkind HS. SARS-CoV-2 infection of the placenta. Journal Of Clinical Investigation 2020, 130: 4947-4953. PMID: 32573498, PMCID: PMC7456249, DOI: 10.1172/jci139569.Peer-Reviewed Case Reports and Technical NotesMeSH KeywordsAbortion, TherapeuticAbruptio PlacentaeAdultBetacoronavirusCoronavirus InfectionsCOVID-19FemaleHumansMicroscopy, Electron, TransmissionPandemicsPhylogenyPlacentaPneumonia, ViralPre-EclampsiaPregnancyPregnancy Complications, InfectiousPregnancy Trimester, SecondRNA, ViralSARS-CoV-2Viral LoadConceptsSevere acute respiratory syndrome coronavirus 2Acute respiratory syndrome coronavirus 2SARS-CoV-2 infectionRespiratory syndrome coronavirus 2SARS-CoV-2 invasionMaternal antibody responseSymptomatic COVID-19Second trimester pregnancySyndrome coronavirus 2Coronavirus disease 2019Materno-fetal interfaceDense macrophage infiltratesPlacental abruptionSevere preeclampsiaMacrophage infiltratesSevere morbidityTrimester pregnancyPregnant womenCoronavirus 2Antibody responseBackgroundThe effectsDisease 2019Histological examinationImmunohistochemical assaysPlacentaCrosstalk between maternal perinatal obesity and offspring dopaminergic circuitry
Yasumoto Y, Horvath TL. Crosstalk between maternal perinatal obesity and offspring dopaminergic circuitry. Journal Of Clinical Investigation 2020, 130: 3416-3418. PMID: 32510474, PMCID: PMC7324168, DOI: 10.1172/jci138123.Peer-Reviewed Original ResearchConceptsMedium spiny neuronsHigh-fat dietMaternal obesityD1 medium spiny neuronsD2 medium spiny neuronsFetal brain developmentDopamine midbrain neuronsBehavioral phenotypesAltered excitatoryPerinatal obesityMaternal miceInhibitory balanceSpiny neuronsDopaminergic circuitryMidbrain neuronsBrain developmentObesityAdult HealthOffspring developmentNeuronsPhenotypeExcitatoryMice
2019
The role of fatty acid binding protein 7 in spinal cord astrocytes in a mouse model of experimental autoimmune encephalomyelitis
Kamizato K, Sato S, Shil SK, Umaru BA, Kagawa Y, Yamamoto Y, Ogata M, Yasumoto Y, Okuyama Y, Ishii N, Owada Y, Miyazaki H. The role of fatty acid binding protein 7 in spinal cord astrocytes in a mouse model of experimental autoimmune encephalomyelitis. Neuroscience 2019, 409: 120-129. PMID: 31051217, DOI: 10.1016/j.neuroscience.2019.03.050.Peer-Reviewed Original ResearchConceptsExperimental autoimmune encephalomyelitisSpinal cordAutoimmune encephalomyelitisMultiple sclerosisWT miceMouse modelFabp7 KO miceSpinal cord astrocytesLumbar spinal cordInduction of inflammationWild-type miceCentral nervous systemProtein 7Role of FABP7Fatty acid homeostasisMRNA expression levelsLate phaseEAE onsetEAE symptomsAstrocyte activationClinical scoresFatty acidsInflammatory cytokinesMyelin degenerationType mice
2016
Inhibition of Fatty Acid Synthase Decreases Expression of Stemness Markers in Glioma Stem Cells
Yasumoto Y, Miyazaki H, Vaidyan LK, Kagawa Y, Ebrahimi M, Yamamoto Y, Ogata M, Katsuyama Y, Sadahiro H, Suzuki M, Owada Y. Inhibition of Fatty Acid Synthase Decreases Expression of Stemness Markers in Glioma Stem Cells. PLOS ONE 2016, 11: e0147717. PMID: 26808816, PMCID: PMC4726602, DOI: 10.1371/journal.pone.0147717.Peer-Reviewed Original ResearchConceptsFatty acid synthaseGlioma stem cellsStem cellsCellular lipid metabolismCellular metabolic changesKey lipogenic enzymesPatient-derived glioma stem cellsLipid biosynthesisFatty acid binding proteinAcid binding proteinPharmacological inhibitorsSynthesis assayBinding proteinAcid synthaseCerulenin treatmentGlial fibrillary acidic protein (GFAP) expressionGSC stemnessProtein expressionCancer cellsDe novo lipogenesisLipogenic enzymesImmunocytochemical analysisMarker nestinStemness markersDecrease expression
2014
Fatty Acid Binding Protein 3 Is Involved in n–3 and n–6 PUFA Transport in Mouse Trophoblasts
Islam A, Kagawa Y, Sharifi K, Ebrahimi M, Miyazaki H, Yasumoto Y, Kawamura S, Yamamoto Y, Sakaguti S, Sawada T, Tokuda N, Sugino N, Suzuki R, Owada Y. Fatty Acid Binding Protein 3 Is Involved in n–3 and n–6 PUFA Transport in Mouse Trophoblasts. Journal Of Nutrition 2014, 144: 1509-1516. PMID: 25122651, DOI: 10.3945/jn.114.197202.Peer-Reviewed Original ResearchConceptsWild-type micePlacental fatty acid transportFatty Acid Binding Protein 3Glucose uptakeLinoleic acidAdult metabolic diseaseBinding protein 3Fetal developmental disordersGene-ablated miceFA transportWild-type fetusesEmbryonic day 15.5Palmitic acidPlacental unitFatty acid transportMetabolic diseasesFetal developmentTrophoblast cellsBeWo cellsProtein 3Day 15.5MiceMouse placentaPlacentaMouse trophoblast
2013
Fatty acid binding protein 7 as a marker of glioma stem cells
Morihiro Y, Yasumoto Y, Vaidyan LK, Sadahiro H, Uchida T, Inamura A, Sharifi K, Ideguchi M, Nomura S, Tokuda N, Kashiwabara S, Ishii A, Ikeda E, Owada Y, Suzuki M. Fatty acid binding protein 7 as a marker of glioma stem cells. Pathology International 2013, 63: 546-553. PMID: 24274717, DOI: 10.1111/pin.12109.Peer-Reviewed Original ResearchConceptsGlioblastoma stem cellsPatient-derived GSC linesGlioma surgical specimensExpression of FABP7Stem cellsAggressive brain tumorGlioma stem cellsNeural stem cellsPoor prognosisFatty acidsMajority of glioblastomasSurgical specimensFABP7 expressionAnaplastic astrocytomaBrain tumorsDiffuse astrocytomasGlioma diagnosisTumor cellsFABP7Western blottingAddition of serumKnown markerGlioblastomaProtein 7GSC linesDifferential expression and regulatory roles of FABP5 and FABP7 in oligodendrocyte lineage cells
Sharifi K, Ebrahimi M, Kagawa Y, Islam A, Tuerxun T, Yasumoto Y, Hara T, Yamamoto Y, Miyazaki H, Tokuda N, Yoshikawa T, Owada Y. Differential expression and regulatory roles of FABP5 and FABP7 in oligodendrocyte lineage cells. Cell And Tissue Research 2013, 354: 683-695. PMID: 24114376, DOI: 10.1007/s00441-013-1730-7.Peer-Reviewed Original ResearchConceptsLineage cellsOligodendrocyte lineage cellsOligodendrocyte progenitor cellsCultured oligodendrocyte progenitor cellsCell populationsOPCs/oligodendrocytesSignal transductionGene transcriptionKey intracellular moleculesOligodendrocyte lineage markersExpression of FABP5Differential expressionRegulatory roleIntracellular moleculesDifferentiation mediumFABP7FABP5FABP7 expressionLineage markersProgenitor cellsOligodendrocyte lineageMixed cortical culturesBrdU uptakeExpressionCentral role