2024
Protein oxidation of fucose environments (POFE) reveals fucose–protein interactions
Xie Y, Chen S, Alvarez M, Sheng Y, Li Q, Maverakis E, Lebrilla C. Protein oxidation of fucose environments (POFE) reveals fucose–protein interactions. Chemical Science 2024, 15: 5256-5267. PMID: 38577366, PMCID: PMC10988611, DOI: 10.1039/d3sc06432h.Peer-Reviewed Original ResearchProtein-protein interactionsCopper-catalyzed click chemistryFormation of hydroxyl radicalsCell membrane glycoproteinMembrane glycoproteinPost-translational modificationsLiquid chromatography coupled with tandem mass spectrometryTandem mass spectrometryClick chemistryBinding partnersGalectin-3-binding proteinProtein functionCellular processesMammalian cellsMass spectrometryOxidized peptidesFucose residuesHydroxyl radicalsLabeled probesBioorthogonal groupsLC-MS/MSAmino acidsProteinFucoseProtein oxidation
2023
Analysis of Cell Glycogen with Quantitation and Determination of Branching Using Liquid Chromatography–Mass Spectrometry
Chen S, Bouchibti Y, Xie Y, Chen Y, Chang V, Lebrilla C. Analysis of Cell Glycogen with Quantitation and Determination of Branching Using Liquid Chromatography–Mass Spectrometry. Analytical Chemistry 2023, 95: 12884-12892. PMID: 37584460, DOI: 10.1021/acs.analchem.3c02230.Peer-Reviewed Original ResearchConceptsUHPLC/QqQ-MSDegree of branchingUltra-high-performance liquid chromatography/triple quadrupole mass spectrometryLiquid chromatography/triple quadrupole mass spectrometryQuadrupole mass spectrometryLiquid chromatography-mass spectrometryChromatography-mass spectrometryPMP derivatizationStructure determinationMass spectrometryDetermination of branchesHep G2) cellsLinkage analysisGlycogen phenotypeToxic to cellsBiological samplesHuman hepatocellular carcinomaBiological functionsSucrose gradientsEnzyme resultsGlucose residuesEffect of insulinSpectrometryInsulin-resistant statesFunction of glycogen