2024
Gly-β-MCA is a potent anti-cholestasis agent against “human-like” hydrophobic bile acid-induced biliary injury in mice
Hasan M, Wang H, Luo W, Clayton Y, Gu L, Du Y, Palle S, Chen J, Li T. Gly-β-MCA is a potent anti-cholestasis agent against “human-like” hydrophobic bile acid-induced biliary injury in mice. Journal Of Lipid Research 2024, 65: 100649. PMID: 39306039, PMCID: PMC11526081, DOI: 10.1016/j.jlr.2024.100649.Peer-Reviewed Original ResearchUrsodeoxycholic acid treatmentUrsodeoxycholic acidPortal fibrosisHepatobiliary injuryClinically relevant dosesMuricholic acidsChronic liver diseaseBile acidsEndogenous bile acidsHydrophobic bile acidsPortal inflammationBile acid pool compositionLithocholic acidTherapeutic optionsBiliary injuryKO miceLine drugsBile acid pool sizeSerum transaminasesBile acid poolRelevant dosesDuctular reactionLiver diseaseCholestasisCholestasis modelThe crosstalk between macrophages and cancer cells potentiates pancreatic cancer cachexia
Liu M, Ren Y, Zhou Z, Yang J, Shi X, Cai Y, Arreola A, Luo W, Fung K, Xu C, Nipp R, Bronze M, Zheng L, Li Y, Houchen C, Zhang Y, Li M. The crosstalk between macrophages and cancer cells potentiates pancreatic cancer cachexia. Cancer Cell 2024, 42: 885-903.e4. PMID: 38608702, PMCID: PMC11162958, DOI: 10.1016/j.ccell.2024.03.009.Peer-Reviewed Original ResearchConceptsPancreatic cancer cachexiaTumor cellsCancer cachexiaTherapeutic targetLimited treatment optionsPancreatic cancer cellsImmune microenvironmentCCL2/CCR2 axisPotential therapeutic targetTreatment optionsMuscle wastingReprogram macrophagesTumorMuscle atrophyCachexiaCancer cellsMacrophagesNon-autonomous activationMuscle remodelingCancerMuscle degradationSecretionCellsMuscleTWEAK
2023
Clinical Features and Management of Acute and Chronic Radiation-Induced Colitis and Proctopathy
Abu-Sbeih H, Tang T, Ali F, Ma W, Shatila M, Luo W, Tan D, Tang C, Richards D, Ge P, Thomas A, Wang Y. Clinical Features and Management of Acute and Chronic Radiation-Induced Colitis and Proctopathy. Cancers 2023, 15: 3160. PMID: 37370770, PMCID: PMC10296205, DOI: 10.3390/cancers15123160.Peer-Reviewed Original ResearchArgon plasma coagulationRadiation therapyMedical managementAPC treatmentSingle tertiary cancer centerRadiation-induced colitisManagement of AcuteNon-bloody diarrheaRetrospective observational studyTertiary cancer centerCancer patients' qualityLogistic regression analysisClinical characteristicsEndoscopic therapySymptom onsetPredominant symptomClinical featuresEndoscopic characteristicsPatients' qualityCancer CenterClinical variablesBloody diarrheaCancer patientsFemale sexFrequent findingAbsence of either Ripk3 or Mlkl reduces incidence of hepatocellular carcinoma independent of liver fibrosis
Mohammed S, Thadathil N, Ohene-Marfo P, Tran A, Van Der Veldt M, Georgescu C, Oh S, Nicklas E, Wang D, Haritha N, Luo W, Janknecht R, Miller B, Wren J, Freeman W, Deepa S. Absence of either Ripk3 or Mlkl reduces incidence of hepatocellular carcinoma independent of liver fibrosis. Molecular Cancer Research 2023, 21: 933-946. PMID: 37204757, PMCID: PMC10472095, DOI: 10.1158/1541-7786.mcr-22-0820.Peer-Reviewed Original ResearchConceptsNonalcoholic fatty liver diseaseProgression of NAFLDHepatocellular carcinomaChronic inflammationLiver fibrosisMale miceMouse modelCholine-deficient high-fat dietFemale wild-type miceOncogenic pathwaysFatty liver diseaseMarkers of inflammationHigh-fat dietLow-fat dietDevelopment of inflammationValid therapeutic targetWild-type miceHepatic inflammationInflammation contributesLiver diseaseWT miceFemale miceSex-specific differencesInflammationTherapeutic targetCombining ASBT inhibitor and FGF15 treatments enhances therapeutic efficacy against cholangiopathy in female but not male Cyp2c70 KO mice
Hasan M, Chen J, Matye D, Wang H, Luo W, Gu L, Clayton Y, Du Y, Li T. Combining ASBT inhibitor and FGF15 treatments enhances therapeutic efficacy against cholangiopathy in female but not male Cyp2c70 KO mice. Journal Of Lipid Research 2023, 64: 100340. PMID: 36737039, PMCID: PMC9986646, DOI: 10.1016/j.jlr.2023.100340.Peer-Reviewed Original ResearchConceptsBile acid poolKO miceTherapeutic efficacyHepatobiliary injuryBile acid pool sizeBile acid exposureGut barrier integrityGut barrier functionBile acid metabolismAcid poolBile acid synthesisAcid pool sizeBile acid uptakePortal inflammationPortal fibrosisGut exposureDuctular reactionGSK2330672Therapeutic reductionUrsodeoxycholic acidMuricholic acidAcid exposureASBT inhibitorSevere cholangiopathyBarrier integrity
2022
17α-estradiol, a lifespan-extending compound, attenuates liver fibrosis by modulating collagen turnover rates in male mice
Ali Mondal S, Sathiaseelan R, Mann S, Kamal M, Luo W, Saccon T, Isola J, Peelor F, Li T, Freeman W, Miller B, Stout M. 17α-estradiol, a lifespan-extending compound, attenuates liver fibrosis by modulating collagen turnover rates in male mice. AJP Endocrinology And Metabolism 2022, 324: e120-e134. PMID: 36516471, PMCID: PMC9902223, DOI: 10.1152/ajpendo.00256.2022.Peer-Reviewed Original ResearchConceptsLiver fibrosisMale miceCombination hormone replacement therapyMatrix metalloproteinase-2 activityHepatic stellate cell activationChronic liver diseaseHormone replacement therapySubset of womenMetalloproteinase-2 activityStellate cell activationGrowth factor-β1Proinflammatory macrophage activationFibrotic burdenCollagen synthesis ratesChronic treatmentLiver diseaseReplacement therapyCytokine expressionMacrophage contentImmune cellsTGF-β1Estrogen signalingHSC activationFactor-β1Macrophage activationmiR-130b/301b Is a Negative Regulator of Beige Adipogenesis and Energy Metabolism In Vitro and In Vivo.
Luo W, Kim Y, Jensen M, Herlea-Pana O, Wang W, Rudolph M, Friedman J, Chernausek S, Jiang S. miR-130b/301b Is a Negative Regulator of Beige Adipogenesis and Energy Metabolism In Vitro and In Vivo. Diabetes 2022, 71: 2360-2371. PMID: 36001751, PMCID: PMC9630090, DOI: 10.2337/db22-0205.Peer-Reviewed Original ResearchConceptsBeige adipogenesisMiR-301bMiR-130bPeroxisome proliferator-activated receptor γ coactivator 1αProliferator-activated receptor γ coactivator 1αImproved glucose toleranceReceptor γ coactivator 1αLess weight gainPotential therapeutic targetCold-induced energy expenditureΓ coactivator 1αMitochondrial biogenesisMetabolic complicationsVisceral adiposityGlucose toleranceThermogenic brownCounteract obesityMetabolic disordersTherapeutic targetAdipose tissueBeige phenotypeMetabolic diseasesAdipose progenitor cellsBeige adipocytesCoactivator 1αSenolytic treatment reduces cell senescence and necroptosis in Sod1 knockout mice that is associated with reduced inflammation and hepatocellular carcinoma
Thadathil N, Selvarani R, Mohammed S, Nicklas E, Tran A, Kamal M, Luo W, Brown J, Lawrence M, Borowik A, Miller B, Van Remmen H, Richardson A, Deepa S. Senolytic treatment reduces cell senescence and necroptosis in Sod1 knockout mice that is associated with reduced inflammation and hepatocellular carcinoma. Aging Cell 2022, 21: e13676. PMID: 35869934, PMCID: PMC9381894, DOI: 10.1111/acel.13676.Peer-Reviewed Original ResearchConceptsSod1KO miceHepatocellular carcinomaQ treatmentCellular senescenceSenescence-associated secretory phenotype factorsMarkers of necroptosisSod1 knockout miceChronic liver diseaseMarkers of inflammationMonths of ageExpression of p16WT levelsSenolytic treatmentLiver diseaseReduced inflammationCu/Zn-superoxide dismutaseMacrophage levelsLiver fibrosisPhenotype factorsLiver cancerNecrostatin-1sKnockout miceAge-associated pathologiesMice nullInflammationAcetyl-Coenzyme A Synthetase 2 Potentiates Macropinocytosis and Muscle Wasting Through Metabolic Reprogramming in Pancreatic Cancer
Zhou Z, Ren Y, Yang J, Liu M, Shi X, Luo W, Fung K, Xu C, Bronze M, Zhang Y, Houchen C, Li M. Acetyl-Coenzyme A Synthetase 2 Potentiates Macropinocytosis and Muscle Wasting Through Metabolic Reprogramming in Pancreatic Cancer. Gastroenterology 2022, 163: 1281-1293.e1. PMID: 35777482, PMCID: PMC9613512, DOI: 10.1053/j.gastro.2022.06.058.Peer-Reviewed Original ResearchConceptsMetabolic reprogrammingDynamin 2Transcriptional activationShort palindromic repeat-associated protein 9 (CRISPR/Cas9) systemSingle-cell RNA sequencing dataRNA sequencing dataProtein 9 (Cas9) systemGlycogen synthase kinasePancreatic cancerEpigenetic reprogrammingFamily member 2Chromatin immunoprecipitationMuscle wastingDownstream targetsSequencing dataSyndecan-1ReprogrammingSynthase kinaseWorse prognosisMacropinocytosisZIP4ACSS2Mouse modelProtein 4Uptake assaysCalcifying nested stromal-epithelial tumor of the liver with recurrence in a transplanted liver – A clinical report with literature review
Kamal M, Atwi D, Gatalica Z, Luo W. Calcifying nested stromal-epithelial tumor of the liver with recurrence in a transplanted liver – A clinical report with literature review. Human Pathology Reports 2022, 28: 300620. DOI: 10.1016/j.hpr.2022.300620.Peer-Reviewed Original ResearchStromal-epithelial tumorAggressive clinical courseSmall blue cellsTERT promoter mutationsRare liver neoplasmLiver transplantationClinical courseFemale predominanceLiver failureTransplanted liverRare tumorRecurrence 3Unknown histogenesisImmunohistochemical spectrumLiver neoplasmsDiffuse positivityClinical reportsTumorsBlue cellsYoung adultsCNSETPromoter mutationsMolecular featuresPIK3CAPathologic mutationsToxicity Assessment of Mesoporous Silica Nanoparticles upon Intravenous Injection in Mice: Implications for Drug Delivery
MacCuaig W, Samykutty A, Foote J, Luo W, Filatenkov A, Li M, Houchen C, Grizzle W, McNally L. Toxicity Assessment of Mesoporous Silica Nanoparticles upon Intravenous Injection in Mice: Implications for Drug Delivery. Pharmaceutics 2022, 14: 969. PMID: 35631554, PMCID: PMC9148138, DOI: 10.3390/pharmaceutics14050969.Peer-Reviewed Original ResearchPre-existing lesionsIntravenous injectionChronic injectionsTreatment groupsBlood chemistryComplete blood count analysisStandard blood chemistryBlood count analysisMultiple intravenous injectionsModerate exacerbationsAdministered treatmentVascular conditionsMinor exacerbationsVital organsFurther evaluationTreatment of diseasesMinimal toxicityTranslational potentialNano-delivery systemsExacerbationFurther studiesInjectionLesionsMiceVivo toxicityCircular RNA ANAPC7 Inhibits Tumor Growth and Muscle Wasting via PHLPP2–AKT–TGF-β Signaling Axis in Pancreatic Cancer
Shi X, Yang J, Liu M, Zhang Y, Zhou Z, Luo W, Fung K, Xu C, Bronze M, Houchen C, Li M. Circular RNA ANAPC7 Inhibits Tumor Growth and Muscle Wasting via PHLPP2–AKT–TGF-β Signaling Axis in Pancreatic Cancer. Gastroenterology 2022, 162: 2004-2017.e2. PMID: 35176309, PMCID: PMC10428768, DOI: 10.1053/j.gastro.2022.02.017.Peer-Reviewed Original ResearchConceptsZinc-dependent transcription factorsFunction of circRNAsMiR-373Dephosphorylation of AktDephosphorylation of CREBNovel tumor suppressorCyclin D1Feed-forward loopMouse skeletal muscleHuman pancreatic cancer cellsRNA interactionsTumor growthTranscription factorsCircular RNAsPancreatic cancer progressionMuscle wastingAkt dephosphorylationRNA immunoprecipitationCancer cell proliferationDownstream targetsPancreatic cancer cellsTumor suppressorSilico analysisPancreatic cancerSignaling AxisExpression and Potential Prognostic Value of SOX9, MCL-1 and SPOCK1 in Gastric Adenocarcinoma
Luo W, Nagaria T, Sun H, Ma J, Lombardo J, Bassett R, Cao A, Tan D. Expression and Potential Prognostic Value of SOX9, MCL-1 and SPOCK1 in Gastric Adenocarcinoma. Pathology & Oncology Research 2022, 28: 1610293. PMID: 35221802, PMCID: PMC8863590, DOI: 10.3389/pore.2022.1610293.Peer-Reviewed Original ResearchConceptsGastric cancerPrognostic valueGastric adenocarcinomaMcl-1Pathologic TNM stageDifferent treatment modalitiesCancer-related deathPotential prognostic valueFurther subgroup analysisNegative prognostic markerTumor heterogeneityDifferent molecular alterationsSOX9 expressionSPOCK1 expressionPrognostic roleCommon malignancyPoor prognosisTNM stageTreatment modalitiesSubgroup analysisPrognostic markerUnivariate analysisDisease prognosisDivergent differentiationMolecular alterations
2021
NUTM1-Rearranged Neoplasms—A Heterogeneous Group of Primitive Tumors with Expanding Spectrum of Histology and Molecular Alterations—An Updated Review
Luo W, Stevens T, Stafford P, Miettinen M, Gatalica Z, Vranic S. NUTM1-Rearranged Neoplasms—A Heterogeneous Group of Primitive Tumors with Expanding Spectrum of Histology and Molecular Alterations—An Updated Review. Current Oncology 2021, 28: 4485-4503. PMID: 34898574, PMCID: PMC8628659, DOI: 10.3390/curroncol28060381.Peer-Reviewed Original ResearchConceptsGenome-wide histone modificationsPost-meiotic spermatidsExpression of oncogenesTumor suppressor geneTranscription regulationHistone modificationsBromodomain proteinsNuclear proteinsRNA sequencingFusion proteinProtein productsSuppressor geneFusion partnerGenesSitu hybridizationDifferent clinical coursesProteinSkin adnexal tumorsPrimitive morphologyOncogenic driversInhibitor therapyClinical courseMolecular alterationsPrimitive tumorHematologic malignanciesCombined ASBT Inhibitor and FGF15 Treatment Improves Therapeutic Efficacy in Experimental Nonalcoholic Steatohepatitis
Matye D, Wang H, Luo W, Sharp R, Chen C, Gu L, Jones K, Ding W, Friedman J, Li T. Combined ASBT Inhibitor and FGF15 Treatment Improves Therapeutic Efficacy in Experimental Nonalcoholic Steatohepatitis. Cellular And Molecular Gastroenterology And Hepatology 2021, 12: 1001-1019. PMID: 33965587, PMCID: PMC8346663, DOI: 10.1016/j.jcmgh.2021.04.013.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBile Acids and SaltsCholesterolCombined Modality TherapyDependovirusDisease Models, AnimalFibroblast Growth FactorsFructoseGene Expression ProfilingGene Expression RegulationGenetic TherapyGenetic VectorsMaleMethylaminesMiceNon-alcoholic Fatty Liver DiseaseThiazepinesTreatment OutcomeConceptsNonalcoholic steatohepatitisBile acid synthesisTherapeutic efficacyFGF19 analogueASBT inhibitorTreatment-associated adverse eventsFecal bile acid excretionASBT inhibitionDiet-induced nonalcoholic steatohepatitisHepatic bile acid synthesisWeight lossBile acid excretionGrowth factor 15Experimental nonalcoholic steatohepatitisDiet-fed miceFurther clinical studiesNew treatment strategiesIntestinal lipid absorptionAdverse eventsAdipose inflammationMetabolic improvementTreatment attenuatesLipid malabsorptionClinical findingsTreatment strategiesAn integrated model of N6-methyladenosine regulators to predict tumor aggressiveness and immune evasion in pancreatic cancer
Zhou Z, Zhang J, Xu C, Yang J, Zhang Y, Liu M, Shi X, Li X, Zhan H, Chen W, McNally L, Fung K, Luo W, Houchen C, He Y, Zhang C, Li M. An integrated model of N6-methyladenosine regulators to predict tumor aggressiveness and immune evasion in pancreatic cancer. EBioMedicine 2021, 65: 103271. PMID: 33714027, PMCID: PMC7966986, DOI: 10.1016/j.ebiom.2021.103271.Peer-Reviewed Original ResearchConceptsImmune checkpoint inhibitorsPancreatic ductal adenocarcinomaImmune evasionCheckpoint inhibitorsOverall survivalImmune infiltrationPancreatic cancerTumor aggressivenessM6A regulatorsSomatic copy number alterationsT cell exhaustionDismal overall survivalMutation profilesN6-methyladenosine regulatorsHigh response ratePancreatic cancer prognosisClassical pancreatic ductal adenocarcinomaCancer CenterColorectal cancerTumor recurrenceDuctal adenocarcinomaM6AscoreBreast cancerTreatment responseCell exhaustionZinc-Dependent Regulation of ZEB1 and YAP1 Coactivation Promotes Epithelial-Mesenchymal Transition Plasticity and Metastasis in Pancreatic Cancer
Liu M, Zhang Y, Yang J, Zhan H, Zhou Z, Jiang Y, Shi X, Fan X, Zhang J, Luo W, Fung K, Xu C, Bronze M, Houchen C, Li M. Zinc-Dependent Regulation of ZEB1 and YAP1 Coactivation Promotes Epithelial-Mesenchymal Transition Plasticity and Metastasis in Pancreatic Cancer. Gastroenterology 2021, 160: 1771-1783.e1. PMID: 33421513, PMCID: PMC8035249, DOI: 10.1053/j.gastro.2020.12.077.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsCation Transport ProteinsCell Line, TumorCell MovementCell PlasticityEpithelial-Mesenchymal TransitionGene Expression Regulation, NeoplasticHumansIntegrin alpha3MiceMicroRNAsNeoplasm InvasivenessNeoplasm MetastasisPancreatic NeoplasmsSignal TransductionSpheroids, CellularTranscription FactorsYAP-Signaling ProteinsZincZinc Finger E-box-Binding Homeobox 1ConceptsEMT plasticityHuman pancreatic cancer cellsPancreatic cancer cellsZinc-dependent regulationHippo pathway effector YAP1Cancer cellsMiR-373Zinc transporter ZIP4Potent transcriptional coactivatorMesenchymal-epithelial transition (MET) statesAssociate domainTranscriptional regulationChromatin immunoprecipitationEffector YAP1Transcriptional coactivatorYAP1/Downstream targetsPancreatic cancer metastasisMouse cellsMolecular eventsZIP4YAP1Cell adhesionLuciferase reporterSpontaneous mouse model
2020
Solitary Fibrous Tumor of Pancreas With Unusual Features: A Case Report
Afzal A, Maldonado-Vital M, Khan S, Farooque U, Luo W. Solitary Fibrous Tumor of Pancreas With Unusual Features: A Case Report. Cureus 2020, 12: e10833. PMID: 33173639, PMCID: PMC7647364, DOI: 10.7759/cureus.10833.Peer-Reviewed Original ResearchPancreatic solitary fibrous tumorSolitary fibrous tumorGastrointestinal stromal tumorsFibrous tumorDiagnosis of SFTCommon neuroendocrine tumorMalignant clinical courseCluster of differentiationPotential diagnostic pitfallMajority of SFTsClinical courseBiopsied specimensStromal tumorsImmunohistochemical featuresVascular tumorsCase reportNeuroendocrine tumorsDiagnostic challengeDedifferentiated liposarcomaPathological spectrumAnatomic sitesDiagnostic pitfallsFocal positivityHypercellular areasAtypical featuresGenomic and single cell sequencing facilitate the dissection of heterogeneity of pancreatic tumors
Edil B, Luo W, Li M. Genomic and single cell sequencing facilitate the dissection of heterogeneity of pancreatic tumors. BMC Medicine 2020, 18: 177. PMID: 32635908, PMCID: PMC7341579, DOI: 10.1186/s12916-020-01637-3.Peer-Reviewed Original ResearchRole of miR‐130b/301b cluster in macrophage polarization and obesity
Jiang S, McBride A, Luo W, Chernausek S. Role of miR‐130b/301b cluster in macrophage polarization and obesity. The FASEB Journal 2020, 34: 1-1. DOI: 10.1096/fasebj.2020.34.s1.09682.Peer-Reviewed Original ResearchDevelopment of obesityMacrophage polarizationKnockout miceMacrophage M1/M2 polarizationM1/M2 polarizationPro-inflammatory activationWhole-body energy expenditureHigh-fat dietEnergy expenditureAlternative macrophage activationWild-type miceWhite adipose tissueBody energy expenditureMacrophage activation statusBone marrow macrophagesPresent studyFat dietIL-4M2 polarizationType miceAlternative activationMacrophage activationAdipose tissueMetabolic diseasesActivation status