2002
A protein kinase A–dependent molecular switch in synapsins regulates neurite outgrowth
Kao HT, Song HJ, Porton B, Ming GL, Hoh J, Abraham M, Czernik AJ, Pieribone VA, Poo MM, Greengard P. A protein kinase A–dependent molecular switch in synapsins regulates neurite outgrowth. Nature Neuroscience 2002, 5: 431-437. PMID: 11976703, DOI: 10.1038/nn840.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesBucladesineCells, CulturedConserved SequenceCyclic AMP-Dependent Protein KinasesEmbryo, NonmammalianEnzyme ActivatorsEnzyme InhibitorsGenes, ReporterMicroinjectionsMolecular Sequence DataMutagenesis, Site-DirectedNeuritesNeuronsPhosphorylationRNA, MessengerSequence Homology, Amino AcidSynapsinsXenopus laevisConceptsProtein kinase ANeurite outgrowthCAMP/protein kinase APhospho-specific antibodiesSingle amino acid residueX. laevis embryosAmino acid residuesDependent molecular switchPhosphorylation of synapsinCyclic AMPEmbryonic neuronal culturesNeuronal cell lineProtein kinaseConstitutive phosphorylationKinase AMolecular approachesMolecular switchAcid residuesLaevis embryosPKA inhibitorPotential molecular approachPhosphorylationGrowth-promoting actionCell linesSynapsin
1999
Regulation of Synaptotagmin I Phosphorylation by Multiple Protein Kinases
Hilfiker S, Pieribone V, Nordstedt C, Greengard P, Czernik A. Regulation of Synaptotagmin I Phosphorylation by Multiple Protein Kinases. Journal Of Neurochemistry 1999, 73: 921-932. PMID: 10461881, DOI: 10.1046/j.1471-4159.1999.0730921.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCalcium-Binding ProteinsCalcium-Calmodulin-Dependent Protein Kinase Type 2Calcium-Calmodulin-Dependent Protein KinasesCasein Kinase IICell DifferentiationClathrinConserved SequenceHumansIsoenzymesMembrane GlycoproteinsMolecular Sequence DataNerve Tissue ProteinsPC12 CellsPeptide MappingPhosphoamino AcidsPhosphorylationProtein Kinase CProtein KinasesProtein Serine-Threonine KinasesRatsSynaptosomesSynaptotagmin ISynaptotagminsConceptsProtein kinase CSynaptotagmin IProtein kinaseKinase IISynaptic vesicle life cycleCasein kinase IIMultiple protein kinasesClathrin-coated vesiclesDependent protein kinase IICalcium-triggered exocytosisProtein kinase IICytoplasmic domainTryptic phosphopeptidesPhosphorylation stateRat synaptotagmin IKinase CCalcium sensorPhosphorylationKinaseSame residuesSynaptic vesiclesPC12 cellsPhorbol esterThr112I phosphorylation