2017
Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight
Platisa J, Vasan G, Yang A, Pieribone VA. Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight. ACS Chemical Neuroscience 2017, 8: 513-523. PMID: 28045247, PMCID: PMC5355904, DOI: 10.1021/acschemneuro.6b00234.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCerebral CortexElectric StimulationEmbryo, MammalianGreen Fluorescent ProteinsHEK293 CellsHumansLuminescent ProteinsMiceModels, MolecularMolecular BiologyMutagenesis, Site-DirectedMutationNeuronsPatch-Clamp TechniquesRecombinant Fusion ProteinsTransfectionVoltage-Sensitive Dye Imaging
2014
Mechanistic Studies of the Genetically Encoded Fluorescent Protein Voltage Probe ArcLight
Han Z, Jin L, Chen F, Loturco JJ, Cohen LB, Bondar A, Lazar J, Pieribone VA. Mechanistic Studies of the Genetically Encoded Fluorescent Protein Voltage Probe ArcLight. PLOS ONE 2014, 9: e113873. PMID: 25419571, PMCID: PMC4242678, DOI: 10.1371/journal.pone.0113873.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAmino AcidsAnimalsCells, CulturedFluorescenceFluorescent DyesGreen Fluorescent ProteinsHEK293 CellsHumansHydrogen-Ion ConcentrationKineticsLuminescent ProteinsMembrane PotentialsMicroscopy, ConfocalMutation, MissenseNeuronsPatch-Clamp TechniquesPrenylationRatsRecombinant Fusion ProteinsSpectrometry, Fluorescence