Rhesus macaque Bcl-6/Bcl-xL B cell immortalization: Discovery of HIV-1 neutralizing antibodies from lymph node
Samsel J, Boswell K, Watkins T, Ambrozak D, Mason R, Yamamoto T, Ko S, Yang Y, Zhou T, Doria-Rose N, Foulds K, Roederer M, Mascola J, Kwong P, Gama L, Koup R. Rhesus macaque Bcl-6/Bcl-xL B cell immortalization: Discovery of HIV-1 neutralizing antibodies from lymph node. Journal Of Immunological Methods 2023, 516: 113445. PMID: 36848985, DOI: 10.1016/j.jim.2023.113445.Peer-Reviewed Original ResearchConceptsB cellsLymph nodesRhesus macaquesHIV-1HIV-1 vaccineExpression of CD40Surface B-cell receptorB-cell immortalizationPre-clinical testingB cell receptorIL-21CD40 ligandAntigen specificityBCL-6Cell receptorIdentification of cellsAntibodiesFunctional assaysCell immortalizationBcl-xLAntigen probeCellsRetroviral vectorsSomatic hypermutationAntibody discovery