2018
High throughput discovery of novel regulators of human ribosome biogenesis
Baserga S, Farley‐Barnes K, McCann K, Ogawa L, Merkel J, Surovtseva Y. High throughput discovery of novel regulators of human ribosome biogenesis. The FASEB Journal 2018, 32: 526.25-526.25. DOI: 10.1096/fasebj.2018.32.1_supplement.526.25.Peer-Reviewed Original ResearchRibosome biogenesisNucleolar functionNumber of nucleoliMammalian cellsGenome-wide siRNA screenRNA polymerase I transcriptionHuman ribosome biogenesisPre-ribosomal RNAPolymerase I transcriptionExperimental Biology 2018 MeetingHigh-throughput discoveryNucleolar proteinsRibosomal DNAProtein regulatorsI transcriptionNew regulatorNovel regulatorSiRNA screenBiogenesisHuman cellsProteinRegulatorNucleoliFASEB JournalThroughput discovery
2016
Nop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA
Zhang J, McCann KL, Qiu C, Gonzalez LE, Baserga SJ, Hall TM. Nop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA. Nature Communications 2016, 7: 13085. PMID: 27725644, PMCID: PMC5062617, DOI: 10.1038/ncomms13085.Peer-Reviewed Original ResearchConceptsEukaryotic ribosome biogenesisCorrect subcellular locationRibosome assembly factorsPre-ribosomal RNAPumilio repeatsRibosome biogenesisHuman ribosomopathiesAssembly factorsBiogenesis factorsRepeat proteinsMature rRNASubcellular locationNop9RNA complexCleavage siteRRNATimely cleavageProteinStructural featuresFinal processing stepRibosomopathiesBiogenesisCleavageYeastNucleaseDiscovery of mammalian regulators of ribosome biogenesis
Farley K, McCann K, Merkel J, Surovtseva Y, Baserga S. Discovery of mammalian regulators of ribosome biogenesis. The FASEB Journal 2016, 30 DOI: 10.1096/fasebj.30.1_supplement.594.1.Peer-Reviewed Original ResearchRibosome biogenesisNucleolar functionNumber of nucleoliGenome-wide siRNA screenRNA polymerase I transcriptionPre-ribosomal RNAPolymerase I transcriptionMammalian regulatorsChromatin stateNucleolar proteinsRibosomal DNAMammalian cellsProtein regulatorsI transcriptionNew regulatorSiRNA screenBiogenesisNucleolar numberHuman cellsProteinRegulatorNucleoliNew roleCellsNew pathway