2000
Evidence for coassembly of mutant GABACρ1 with GABAAγ2S, glycine α1 and glycine α2 receptor subunits in vitro
Pan Z, Zhang D, Zhang X, Lipton S. Evidence for coassembly of mutant GABACρ1 with GABAAγ2S, glycine α1 and glycine α2 receptor subunits in vitro. European Journal Of Neuroscience 2000, 12: 3137-3145. PMID: 10998097, DOI: 10.1046/j.1460-9568.2000.00198.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDose-Response Relationship, DrugElectrophysiologyGABA AntagonistsGamma-Aminobutyric AcidGene ExpressionIn Vitro TechniquesMutagenesisNeural InhibitionOocytesPicrotoxinProtein Structure, TertiaryRatsReceptors, GABAReceptors, GABA-AReceptors, GABA-BReceptors, GlycineRetinaXenopus laevisConceptsGlycine alpha1Rho1 subunitAlpha2 subunitHeteromeric receptorsGABA dose-response curveBlockade of GABAVoltage-clamp recordingsGamma-aminobutyric acidXenopus laevis oocyte expression systemTwo-electrode voltage-clamp recordingsDose-response curveResponse propertiesPicrotoxinin sensitivityOocyte expression systemGamma2 subunitGABAReceptor subunitsPharmacological propertiesReceptorsUnique gatingFunctional evidenceGABAAAlpha1Gamma2Homomeric
1997
Agonist-induced closure of constitutively open γ-aminobutyric acid channels with mutated M2 domains
Pan Z, Zhang D, Zhang X, Lipton S. Agonist-induced closure of constitutively open γ-aminobutyric acid channels with mutated M2 domains. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 6490-6495. PMID: 9177245, PMCID: PMC21077, DOI: 10.1073/pnas.94.12.6490.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCloning, MolecularFemaleGABA AgonistsGABA AntagonistsGamma-Aminobutyric AcidIon Channel GatingIon ChannelsMacromolecular SubstancesMembrane PotentialsMolecular Sequence DataMutagenesis, Site-DirectedOocytesOrganophosphorus CompoundsPoint MutationRatsReceptors, GABAReceptors, GlycineReceptors, NicotinicRecombinant ProteinsSequence AlignmentSequence Homology, Amino AcidVirulence Factors, BordetellaXenopus laevisConceptsLigand-gated ion channelsChannel gatingIon channelsAmino acid residuesAminobutyric acid channelsSingle-point mutantsAbsence of ligandChannel pore regionStructure-function relationshipsPoint mutantsAcid channelImportant new insightsAllosteric transitionSubunit resultsAcid residuesM2 domainConstitutive currentReceptor proteinPore regionAgonist bindingAgonist regulationSubstituting alanineM2 regionSpontaneous channel openingChannel opening