1997
Neurotoxicity associated with dual actions of homocysteine at the N-methyl-d-aspartate receptor
Lipton S, Kim W, Choi Y, Kumar S, D’Emilia D, Rayudu P, Arnelle D, Stamler J. Neurotoxicity associated with dual actions of homocysteine at the N-methyl-d-aspartate receptor. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 5923-5928. PMID: 9159176, PMCID: PMC20882, DOI: 10.1073/pnas.94.11.5923.Peer-Reviewed Original ResearchMeSH Keywords2-Amino-5-phosphonovalerate6-Cyano-7-nitroquinoxaline-2,3-dioneAdultAnimalsCells, CulturedCerebral CortexChildDizocilpine MaleateEmbryo, MammalianEvoked PotentialsGlycineHomocysteineHumansKineticsKynurenic AcidNeuronsNeurotoxinsN-MethylaspartateRatsRats, Sprague-DawleyReceptors, N-Methyl-D-AspartateConceptsN-methyl-D-aspartate receptorsGlycine coagonist siteHomocysteine neurotoxicityNeuronal damageHead traumaVascular diseaseTotal homocysteineNeurotoxic concentrationsGlycine levelsCoagonist siteNeuroprotective activityCoagulation systemNervous systemDisease pathogenesisPartial antagonistExcessive Ca2HomocysteineAdverse effectsElevated levelsPathological conditionsDual actionVessel wallReceptorsReactive oxygen generationPathogenesis
1995
N-methyl-d-aspartate receptors are critical for mediating the effects of glutamate on intracellular calcium concentration and immediate early gene expression in cultured hippocampal neurons
Bading H, Segal M, Sucher N, Dudek H, Lipton S, Greenberg M. N-methyl-d-aspartate receptors are critical for mediating the effects of glutamate on intracellular calcium concentration and immediate early gene expression in cultured hippocampal neurons. Neuroscience 1995, 64: 653-664. PMID: 7715778, DOI: 10.1016/0306-4522(94)00462-e.Peer-Reviewed Original ResearchMeSH Keywords2-Amino-5-phosphonovalerate6-Cyano-7-nitroquinoxaline-2,3-dioneAnimalsBlotting, NorthernCalciumCells, CulturedElectrophysiologyGene Expression RegulationGenes, fosGenes, junGlutamic AcidGlyceraldehyde-3-Phosphate DehydrogenasesHippocampusImmunohistochemistryKainic AcidKynurenic AcidNeuronsNifedipineProto-Oncogene Proteins c-fosRatsRats, Inbred StrainsReceptors, N-Methyl-D-AspartateRNA, MessengerConceptsN-methyl-D-aspartate receptorsGene transcriptionCultured hippocampal neuronsD-aspartate receptorsRegulation of transcriptionHippocampal neuronsImmediate early gene transcriptionEarly gene transcriptionImmediate early genesTranscriptional regulationEarly gene expressionCalcium channelsTranscriptional responseCalcium imagingL-type voltage-sensitive calcium channelsN-methyl-D-aspartate (NMDA) subtypeExcitatory amino acid receptorsTranscriptional levelImmediate early gene expressionVoltage-sensitive calcium channelsNuclear runGene expressionL-type calcium channelsAmino acid receptorsRNA analysis
1992
l-Homocysteic acid selectively activates N-methyl-d-aspartate receptors of rat retinal ganglion cells
Zhang D, Lipton S. l-Homocysteic acid selectively activates N-methyl-d-aspartate receptors of rat retinal ganglion cells. Neuroscience Letters 1992, 139: 173-177. PMID: 1351663, DOI: 10.1016/0304-3940(92)90545-i.Peer-Reviewed Original ResearchMeSH Keywords2-Amino-5-phosphonovalerateAnimalsEvoked PotentialsGlycineHomocysteineKynurenic AcidRatsReceptors, N-Methyl-D-AspartateRetinal Ganglion CellsStimulation, ChemicalConceptsRat retinal ganglion cellsRetinal ganglion cellsGanglion cellsRat RGCsN-methyl-D-aspartate receptorsNMDA receptor-channel complexConcentrations of NMDAL-homocysteic acidWhole-cell recordingsReceptor-channel complexRetinal transmittersMaximal responseHomocysteic acidNMDAPatch electrodePossible roleMillimolar concentrationsHCACellsReceptorsResponse
1991
Synergistic effects of HIV coat protein and NMDA receptor-mediated neurotoxicity
Lipton S, Sucher N, Kaiser P, Dreyer E. Synergistic effects of HIV coat protein and NMDA receptor-mediated neurotoxicity. Neuron 1991, 7: 111-118. PMID: 1676893, DOI: 10.1016/0896-6273(91)90079-f.Peer-Reviewed Original ResearchConceptsNMDA receptor-mediated neurotoxicityHIV-1 coat protein gp120Whole-cell patch-clamp recordingsHIV coat proteinRat retinal culturesSubsequent neuronal damagePatch-clamp recordingsGlutamate pyruvate transaminaseNeuronal injuryNeuronal damageEndogenous glutamateGanglion cellsNMDA antagonistsProtein gp120Low picomolar concentrationsRetinal culturesGp120NeurotoxicityComplete protectionMinute increasePicomolar concentrationsGlutamateInjuryAntagonistTransaminaseN-methyl-D-aspartate antagonists prevent kainate neurotoxicity in rat retinal ganglion cells in vitro
Sucher N, Aizenman E, Lipton. N-methyl-D-aspartate antagonists prevent kainate neurotoxicity in rat retinal ganglion cells in vitro. Journal Of Neuroscience 1991, 11: 966-971. PMID: 1672708, PMCID: PMC6575374, DOI: 10.1523/jneurosci.11-04-00966.1991.Peer-Reviewed Original ResearchMeSH Keywords2-Amino-5-phosphonovalerate6-Cyano-7-nitroquinoxaline-2,3-dioneAmino AcidsAnimalsCells, CulturedElectrophysiologyKainic AcidN-MethylaspartateQuinoxalinesRatsRetinal Ganglion CellsConceptsRetinal ganglion cellsNMDA receptorsGanglion cellsEndogenous GluKA receptorsSpecific NMDA receptor antagonistN-methyl-D-aspartate antagonistsRetinal ganglion cell deathRat retinal ganglion cellsDoses of NMDAGanglion cell deathSpecific NMDA antagonistNMDA receptor antagonistOwn specific receptorsPatch-clamp electrophysiology experimentsAddition of kainateMicroM kainateKainate neurotoxicityReceptor antagonistNMDA antagonistsKainateCellular injuryExogenous glutamateSpecific receptorsDetectable inhibition
1990
Comparison of delayed administration of competitive and uncompetitive antagonists in preventing NMDA receptor-mediated neuronal death.
Levy D, Lipton S. Comparison of delayed administration of competitive and uncompetitive antagonists in preventing NMDA receptor-mediated neuronal death. Neurology 1990, 40: 852-5. PMID: 1970428, DOI: 10.1212/wnl.40.5.852.Peer-Reviewed Original ResearchConceptsMK-801Uncompetitive antagonistCompetitive antagonistNMDA receptor-mediated neuronal deathN-methyl-D-aspartate receptorsRetinal ganglion cell neuronsStudies of excitotoxicityGanglion cell neuronsGlutamate-induced injuryNeuronal cell deathUncompetitive NMDA antagonistsHypoxia-ischemiaNeurologic injuryProtective dosesNeuronal deathCentral neuronsInitial insultNMDA antagonistsProtective effectCell neuronsAntagonistMinutes of exposureToxic damageInjuryCell deathRedox modulation of NMDA receptor-mediated toxicity in mammalian central neurons
Levy D, Sucher N, Lipton S. Redox modulation of NMDA receptor-mediated toxicity in mammalian central neurons. Neuroscience Letters 1990, 110: 291-296. PMID: 1970145, DOI: 10.1016/0304-3940(90)90862-4.Peer-Reviewed Original ResearchConceptsCentral neuronsNMDA receptorsN-methyl-D-aspartate (NMDA) subtypeNMDA receptor-mediated toxicityAmyotrophic lateral sclerosis-ParkinsonismAcute neurological injuryGlutamate-induced deathNMDA receptor sitesSurvival of neuronsRetinal ganglion cellsMammalian central neuronsReceptor-operated channelsChronic degenerative diseasesReceptor-mediated toxicityHypoxia-ischemiaNMDA neurotoxicityDementia complexNeurological injurySimilar pathogenesisGanglion cellsGlutamate receptorsEnhanced killingRedox modulationDegenerative diseasesHuntington's disease