1997
Agonist-induced closure of constitutively open γ-aminobutyric acid channels with mutated M2 domains
Pan Z, Zhang D, Zhang X, Lipton S. Agonist-induced closure of constitutively open γ-aminobutyric acid channels with mutated M2 domains. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 6490-6495. PMID: 9177245, PMCID: PMC21077, DOI: 10.1073/pnas.94.12.6490.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsCloning, MolecularFemaleGABA AgonistsGABA AntagonistsGamma-Aminobutyric AcidIon Channel GatingIon ChannelsMacromolecular SubstancesMembrane PotentialsMolecular Sequence DataMutagenesis, Site-DirectedOocytesOrganophosphorus CompoundsPoint MutationRatsReceptors, GABAReceptors, GlycineReceptors, NicotinicRecombinant ProteinsSequence AlignmentSequence Homology, Amino AcidVirulence Factors, BordetellaXenopus laevisConceptsLigand-gated ion channelsChannel gatingIon channelsAmino acid residuesAminobutyric acid channelsSingle-point mutantsAbsence of ligandChannel pore regionStructure-function relationshipsPoint mutantsAcid channelImportant new insightsAllosteric transitionSubunit resultsAcid residuesM2 domainConstitutive currentReceptor proteinPore regionAgonist bindingAgonist regulationSubstituting alanineM2 regionSpontaneous channel openingChannel opening
1990
Neural nicotinic acetylcholine responses in sensory neurons from postnatal rat
Sucher N, Cheng T, Lipton S. Neural nicotinic acetylcholine responses in sensory neurons from postnatal rat. Brain Research 1990, 533: 248-254. PMID: 2289141, DOI: 10.1016/0006-8993(90)91346-i.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBungarotoxinsCells, CulturedFluorescent DyesGanglia, SpinalMembrane PotentialsNeurons, AfferentReceptors, NicotinicConceptsDRG cellsPostnatal ratsInward currentsFunctional nicotinic acetylcholine receptorsDorsal root ganglion cellsNicotinic acetylcholine responsesAgonist-induced currentsNicotinic acetylcholine receptorsPatch-clamp techniqueWhole-cell configurationAcetylcholine responseNicotinic responsesGanglion cellsNeuronal bungarotoxinNeuronal typesSensory neuronsNicotinic acetylcholineAcetylcholine receptorsNewborn animalsAcetylcholineIrreversible blockPresence of toxinsRatsCellsDMPPBlockade of nicotinic responses in rat retinal ganglion cells by neuronal bungarotoxin
Aizenman E, Loring R, Lipton S. Blockade of nicotinic responses in rat retinal ganglion cells by neuronal bungarotoxin. Brain Research 1990, 517: 209-214. PMID: 2375991, DOI: 10.1016/0006-8993(90)91028-f.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAnimalsBungarotoxinsCells, CulturedMembrane PotentialsRatsReceptors, NicotinicRetinaRetinal Ganglion CellsConceptsRat retinal ganglion cellsRetinal ganglion cellsNeuronal bungarotoxinGanglion cellsTotal blockadeFunctional nicotinic receptorsAcetylcholine-induced currentsNicotinic acetylcholine responsesNicotinic currentsAcetylcholine responseNicotinic responsesComplete blockadeNicotinic receptorsSuperfusion methodPressure ejectionToxin applicationBlockadePatch electrodeBungarotoxinCellsHigh affinityResponseReceptors
1989
Characterization of nicotinic receptors in chick retina using a snake venom neurotoxin that blocks neuronal nicotinic receptor function
Loring R, Aizenman E, Lipton, Zigmond R. Characterization of nicotinic receptors in chick retina using a snake venom neurotoxin that blocks neuronal nicotinic receptor function. Journal Of Neuroscience 1989, 9: 2423-2431. PMID: 2746336, PMCID: PMC6569765, DOI: 10.1523/jneurosci.09-07-02423.1989.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBungarotoxinsChickensDimethylphenylpiperazinium IodideElectrophysiologyNeuronsNeurotoxinsNicotineReceptors, NicotinicRetinaConceptsNicotinic receptor functionNicotinic receptorsChick retinaNicotinic agonistsReceptor functionNeuronal nicotinic receptor functionIntact chick retinaFunctional nicotinic receptorsInner plexiform layerAutonomic gangliaGanglion cellsPlexiform layerCholine acetyltransferaseNeuronal bungarotoxinNeuromuscular antagonistsNicotinic functionNeuronal preparationsRetinaAgonistsReceptorsAntagonistSnake venom neurotoxinsVenom neurotoxinsLow affinityHigh affinity
1988
Spontaneous release of acetylcholine affects the physiological nicotinic responses of rat retinal ganglion cells in culture
Lipton S. Spontaneous release of acetylcholine affects the physiological nicotinic responses of rat retinal ganglion cells in culture. Journal Of Neuroscience 1988, 8: 3857-3868. PMID: 2903915, PMCID: PMC6569610, DOI: 10.1523/jneurosci.08-10-03857.1988.Peer-Reviewed Original ResearchConceptsRetinal ganglion cellsGanglion cellsEndogenous AChRetinal culturesMammalian retinal ganglion cellsOutward currentsRat retinal ganglion cellsCentral mammalian neuronsCholinergic amacrine cellsWhole-cell recordingsConcentrations of AChTonic currentsNicotinic currentsExogenous AChCholinergic responsesNicotinic antagonistsNicotinic responsesAmacrine cellsCentral neuronsNicotinic agonistsD-turbocurarineRetinal cellsTrophic effectsSpontaneous releaseIntact retinaNicotinic Antagonists Enhance Process Outgrowth by Rat Retinal Ganglion Cells in Culture
Lipton S, Frosch M, Phillips M, Tauck D, Aizenman E. Nicotinic Antagonists Enhance Process Outgrowth by Rat Retinal Ganglion Cells in Culture. Science 1988, 239: 1293-1296. PMID: 3344435, DOI: 10.1126/science.3344435.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAtropineCells, CulturedMecamylaminePicrotoxinRatsReceptors, NicotinicRetinaRetinal Ganglion CellsTubocurarineConceptsRetinal ganglion cellsNicotinic antagonistsGanglion cellsFunctional nicotinic cholinergic receptorsRetinal ganglion cell processesRat retinal ganglion cellsRetinal ganglion cell neuronsNicotinic cholinergic drugsSpecific nicotinic antagonistsProcess outgrowthGanglion cell neuronsNicotinic cholinergic receptorsGanglion cell processesNormal control mechanismsApplied acetylcholinePostsynaptic effectsCholinergic receptorsAmacrine cellsCholinergic drugsPharmacologic blockadeSpontaneous leaksRemoval of inhibitionRetinal culturesAcetylcholineCell neurons