2024
Using in vivo intact structure for system-wide quantitative analysis of changes in proteins
Son A, Kim H, Diedrich J, Bamberger C, McClatchy D, Lipton S, Yates J. Using in vivo intact structure for system-wide quantitative analysis of changes in proteins. Nature Communications 2024, 15: 9310. PMID: 39468068, PMCID: PMC11519357, DOI: 10.1038/s41467-024-53582-x.Peer-Reviewed Original ResearchConceptsAlzheimer's diseaseProtein footprinting methodGlobal expression profilingIn vivo conformationStructural alterations of proteinsCo-expressed proteinsMass spectrometry-based methodsAlterations of proteinsProteostasis dysfunctionSpectrometry-based methodsProtein misfoldingConformation of proteinsStructural changesLysine residuesDynamic structural changesBiological functionsProteomics experimentsDimethyl labelingExpression profilesProtein conformationConformational changesProteinIntact proteinDesign of therapeutic interventionsMeasuring dynamic structural changes
1995
Cloning of a gamma-aminobutyric acid type C receptor subunit in rat retina with a methionine residue critical for picrotoxinin channel block.
Zhang D, Pan Z, Zhang X, Brideau A, Lipton S. Cloning of a gamma-aminobutyric acid type C receptor subunit in rat retina with a methionine residue critical for picrotoxinin channel block. Proceedings Of The National Academy Of Sciences Of The United States Of America 1995, 92: 11756-11760. PMID: 8524843, PMCID: PMC40481, DOI: 10.1073/pnas.92.25.11756.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceChloride ChannelsCloning, MolecularDose-Response Relationship, DrugDrug ResistanceElectric ConductivityGABA AntagonistsIon ChannelsMethionineMolecular Sequence DataMutagenesis, Site-DirectedPicrotoxinProtein ConformationRatsReceptors, GABARetinaSequence Homology, Amino AcidSesterterpenesStructure-Activity RelationshipConceptsGABAC responsesRat retinaRho 2 subunitsGamma-aminobutyric acidPTX resistanceHeteromeric receptorsReceptor subunitsFunctional homomeric receptorsRho 1 receptorsGABA receptor subunitsInhibitory neurotransmissionMechanism of blockGABA receptorsMammalian retinaConvulsant picrotoxininHomomeric receptorsRat receptorRetinaIonotropic receptorsReceptorsChannel blockPicrotoxininSecond membrane-spanning regionNative receptorPredominant determinant