The PPE18 Protein of Mycobacterium tuberculosis Inhibits NF-κB/rel–Mediated Proinflammatory Cytokine Production by Upregulating and Phosphorylating Suppressor of Cytokine Signaling 3 Protein
Nair S, Pandey AD, Mukhopadhyay S. The PPE18 Protein of Mycobacterium tuberculosis Inhibits NF-κB/rel–Mediated Proinflammatory Cytokine Production by Upregulating and Phosphorylating Suppressor of Cytokine Signaling 3 Protein. The Journal Of Immunology 2011, 186: 5413-5424. PMID: 21451109, DOI: 10.4049/jimmunol.1000773.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, BacterialBacterial ProteinsBlotting, WesternCell LineCell SeparationCytokinesElectrophoretic Mobility Shift AssayFlow CytometryHumansImmunoprecipitationInflammationMacrophagesMycobacterium tuberculosisNF-kappa BPhosphorylationReverse Transcriptase Polymerase Chain ReactionRNA, Small InterferingSignal TransductionSuppressor of Cytokine Signaling 3 ProteinSuppressor of Cytokine Signaling ProteinsTranscription Factor RelATransfectionTuberculosisUp-RegulationConceptsTranscription factorsNF-κB/Rel transcription factorsNuclear translocationNF-κB/RelRel transcription factorsSubsequent intracellular survivalC-Rel transcription factorPhosphorylation sitesTyrosine phosphorylationSpecific knockdownSuccessful infectionIntracellular survivalRel subunitsCytokine signaling-3 (SOCS-3) proteinNovel mechanismIκB kinasePhosphorylationProteinNuclear levelsNF-κB activationSOCS3Phosphorylation of IκBαSuppressorTNF-α productionTranslocation