2002
Vitamin B12 Stalls the 80S Ribosomal Complex on the Hepatitis C Internal Ribosome Entry Site
Takyar S, Gowans E, Lott W. Vitamin B12 Stalls the 80S Ribosomal Complex on the Hepatitis C Internal Ribosome Entry Site. Journal Of Molecular Biology 2002, 319: 1-8. PMID: 12051931, DOI: 10.1016/s0022-2836(02)00300-5.Peer-Reviewed Original ResearchConceptsIRES-dependent translationRibosome entry siteHepatitis C virus internal ribosome entry siteInternal ribosome entry siteEntry siteTranslation inhibitor cycloheximideS ribosomal complexCap-dependent mRNAMRNA-specificGradient centrifugation analysisSucrose gradient centrifugation analysisCompetitive binding mechanismHCV IRESRibosomal complexesTranslation inhibitorsInhibitor cycloheximideCentrifugation analysisMRNAComplex formationCycloheximideBinding mechanism
2001
Vitamin B12 and hepatitis C: Molecular biology and human pathology
Lott W, Takyar S, Tuppen J, Crawford D, Harrison M, Sloots T, Gowans E. Vitamin B12 and hepatitis C: Molecular biology and human pathology. Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 4916-4921. PMID: 11296247, PMCID: PMC33138, DOI: 10.1073/pnas.081072798.Peer-Reviewed Original ResearchMeSH KeywordsAntiviral AgentsBase SequenceClassical swine fever virusDose-Response Relationship, DrugEncephalomyocarditis virusHepacivirusHepatitis CHumansLiverMolecular Sequence DataProtein BindingProtein BiosynthesisRegulatory Sequences, Nucleic AcidRibosomesSubstrate SpecificityThermodynamicsViral LoadVirus ReplicationVitamin B 12ConceptsViral loadCobalamin concentrationsHCV viral loadVitamin B12HCV-infected liverDose-dependent mannerReporter gene in vitroEncephalomyocarditis virusGenes in vitroInternal ribosome entry site (IRES)-dependent translationHCVHCV replicationSwine fever virusCap-dependent mechanismSerum cobalamin concentrationsSerumHuman liverHuman serumIRES elementsLiverInhibit translationB12VitaminVirus functions