2016
Whole genome sequencing of Plasmodium falciparum from dried blood spots using selective whole genome amplification
Oyola S, Ariani C, Hamilton W, Kekre M, Amenga-Etego L, Ghansah A, Rutledge G, Redmond S, Manske M, Jyothi D, Jacob C, Otto T, Rockett K, Newbold C, Berriman M, Kwiatkowski D. Whole genome sequencing of Plasmodium falciparum from dried blood spots using selective whole genome amplification. Malaria Journal 2016, 15: 597. PMID: 27998271, PMCID: PMC5175302, DOI: 10.1186/s12936-016-1641-7.Peer-Reviewed Original ResearchConceptsVenous bloodSelective whole genome amplificationMalaria patientsDBS samplesBlood spotsPlasmodium falciparumClinical samplesSymptomatic malaria patientsHigh quality clinical samplesDried Blood SpotsP. falciparum DNAMalaria parasite Plasmodium falciparumHead comparisonParasite Plasmodium falciparumDrug resistance lociHealthcare applicationsPatientsWhole-genome sequencingGenome amplificationDBS extractsSNP concordanceWhole genome amplificationFalciparumCurrent dataScalable way
2015
Association mapping by pooled sequencing identifies TOLL 11 as a protective factor against Plasmodium falciparum in Anopheles gambiae
Redmond S, Eiglmeier K, Mitri C, Markianos K, Guelbeogo W, Gneme A, Isaacs A, Coulibaly B, Brito-Fravallo E, Maslen G, Mead D, Niare O, Traore S, Sagnon N, Kwiatkowski D, Riehle M, Vernick K. Association mapping by pooled sequencing identifies TOLL 11 as a protective factor against Plasmodium falciparum in Anopheles gambiae. BMC Genomics 2015, 16: 779. PMID: 26462916, PMCID: PMC4603968, DOI: 10.1186/s12864-015-2009-z.Peer-Reviewed Original ResearchConceptsGenetic mappingFounder coloniesHigh nucleotide diversityNon-model genomesLow linkage disequilibriumPhenotype-genotype mappingPopulation stratificationParasite infection levelsHuman malaria parasiteGenotyping of SNPsNucleotide diversityPooled sequencesAssociation mappingGenomic lociLinkage mappingPlasmodium falciparumSignificant SNPsMosquito systemMarker densityDNA poolsAnopheles gambiaeNatural variationIndividual mosquitoesLinkage disequilibriumSequencing identifies