2024
TRPM7 facilitates fibroblast-like synoviocyte proliferation, metastasis and inflammation through increasing IL-6 stability via the PKCα-HuR axis in rheumatoid arthritis
Lin Y, Chen Y, Hu W, Liu X, Hao W, Xing J, Ding J, Xu Y, Yao F, Zhao Y, Wang K, Li S, Yu Q, Hu W, Zhou R. TRPM7 facilitates fibroblast-like synoviocyte proliferation, metastasis and inflammation through increasing IL-6 stability via the PKCα-HuR axis in rheumatoid arthritis. International Immunopharmacology 2024, 132: 111933. PMID: 38581988, DOI: 10.1016/j.intimp.2024.111933.Peer-Reviewed Original ResearchConceptsTransient receptor potential melastatin 7Rheumatoid arthritisInhibition of transient receptor potential melastatin 7Human RA patientsSynovial hyperplasiaAdjuvant-induced arthritis ratsIncreased TRPM7 expressionIL-6 mRNATreatment of RAPathogenesis of RAFibroblast-like synoviocytesTRPM7 silencingProgression of rheumatoid arthritisTRPM7 expressionChannel inhibitionCation channelsRA patientsMetastasisPharmacological inhibitionArthritis ratsInflammationNuclear translocationSynoviocyte proliferationHyperplasiaProliferationExtracellular CIRP induces abnormal activation of fibroblast-like synoviocytes from patients with RA via the TLR4-mediated HDAC3 pathways
Yao F, Zhao Y, Yu Q, Hu W, Lin Y, Chen Y, Li L, Sun C, Li S, Wang K, Yang M, Zhou R, Hu W. Extracellular CIRP induces abnormal activation of fibroblast-like synoviocytes from patients with RA via the TLR4-mediated HDAC3 pathways. International Immunopharmacology 2024, 128: 111525. PMID: 38218010, DOI: 10.1016/j.intimp.2024.111525.Peer-Reviewed Original ResearchConceptsExtracellular cold-inducible RNA-binding proteinCold-inducible RNA-binding proteinToll-like receptor 4Histone deacetylase 3Endogenous proinflammatory moleculesRheumatoid arthritisActivity of RA-FLSAA ratsAbnormal activationProinflammatory moleculesEffect of cold-inducible RNA-binding proteinHistone deacetylase 3 knockdownRelease of IL-1bSeverity of arthritisFibroblast-like synoviocytesDevelopment of rheumatoid arthritisRA-FLSActivation of fibroblast-like synoviocytesIL-33Expression of N-cadherinProinflammatory effectsArthritis severityIL-1BInflammatory diseasesReceptor 4
2022
Acid-sensitive ion channel 1a mediates osteoarthritis chondrocyte senescence by promoting Lamin B1 degradation
Ding J, Chen Y, Zhao Y, Chen F, Dong L, Zhang H, Hu W, Li S, Zhou R, Hu W. Acid-sensitive ion channel 1a mediates osteoarthritis chondrocyte senescence by promoting Lamin B1 degradation. Biochemical Pharmacology 2022, 202: 115107. PMID: 35643339, DOI: 10.1016/j.bcp.2022.115107.Peer-Reviewed Original ResearchConceptsAcid-sensitive ion channel 1aChondrocyte senescenceIon channel 1aChannel 1aBeclin-1Chronic joint diseaseBeclin-1 protein expressionRat OA modelProtein Beclin 1Senescence-related markersJoint diseaseOA modelAging of chondrocytesJoint cavityPsalmotoxin-1Chondrocyte autophagyOsteoarthritisB1 expressionProtein expressionCationic channelsHairpin RNALamin B1Autophagy pathwayArticular cartilageLamin B1 expressionBlockade of ASIC1a inhibits acid-induced rat articular chondrocyte senescence through regulation of autophagy
Yang Y, Ding J, Chen Y, Ma G, Wei X, Zhou R, Hu W. Blockade of ASIC1a inhibits acid-induced rat articular chondrocyte senescence through regulation of autophagy. Human Cell 2022, 35: 665-677. PMID: 35075624, DOI: 10.1007/s13577-022-00676-7.Peer-Reviewed Original ResearchConceptsAcid-sensitive ion channel 1aChondrocyte senescenceRat articular chondrocytesSenescence-related markersExtracellular acidificationSenescence-associated secretory phenotypeTreatment of osteoarthritisPotential therapeutic strategySenescence-associated β-galactosidaseIon channel 1aProtein LC3B-IIArticular chondrocytesC28/I2 chondrocyte cell lineMatrix metalloproteinases genesChondrocyte cell lineTherapeutic strategiesChondrocyte damageControl groupROS expressionPsalmotoxin-1Secretory phenotypeChannel 1aChondrocyte autophagyLC3B-IIMetalloproteinases genes
2021
Curcumin ameliorates IL‐1β‐induced apoptosis by activating autophagy and inhibiting the NF‐κB signaling pathway in rat primary articular chondrocytes
Chen T, Zhou R, Chen Y, Fu W, Wei X, Ma G, Hu W, Lu C. Curcumin ameliorates IL‐1β‐induced apoptosis by activating autophagy and inhibiting the NF‐κB signaling pathway in rat primary articular chondrocytes. Cell Biology International 2021, 45: 976-988. PMID: 33377585, DOI: 10.1002/cbin.11541.Peer-Reviewed Original ResearchConceptsIL-1βChondrocyte apoptosisProtective effectNF-κBPrimary articular chondrocytesNF-κB inhibitor pyrrolidine dithiocarbamatePhosphorylation levelsArticular chondrocytesCurcumin-mediated protectionWnt/β-catenin inhibitorInhibitor pyrrolidine dithiocarbamateIL-1β stimulationArticular chondrocyte apoptosisArticular cartilage damageΒ-catenin inhibitorMechanism of curcuminArticular cartilage diseaseSynthase kinase-3βCurcumin pretreatmentRheumatoid arthritisΚB p65Chondrocyte injuryInterleukin-1βCartilage damageXAV-939
2020
Acid‐sensing ion channel 1a mediates acid‐induced pyroptosis through calpain‐2/calcineurin pathway in rat articular chondrocytes
Zu S, Feng Y, Zhu C, Wu X, Zhou R, Li G, Dai B, Wang Z, Xie Y, Li Y, Ge J, Chen F. Acid‐sensing ion channel 1a mediates acid‐induced pyroptosis through calpain‐2/calcineurin pathway in rat articular chondrocytes. Cell Biology International 2020, 44: 2140-2152. PMID: 32678496, DOI: 10.1002/cbin.11422.Peer-Reviewed Original ResearchConceptsAcid-sensing ion channel 1aAcid-sensing ion channelsRat articular chondrocytesIon channel 1aChondrocyte pyroptosisTime-dependent mannerCalpain-2Activation of ASICsChannel 1aExtracellular acidosisInhibition of ASIC1aProtein expressionInterleukin-1β levelsArticular chondrocytesSystemic autoimmune diseasePrimary rat articular chondrocytesApoptosis-associated speck-like proteinNLRP3 inflammasome proteinsCalcineurin pathwaySpeck-like proteinCation channel familyRheumatoid arthritisAutoimmune diseasesDegenerative articular cartilageInflammasome proteins
2018
The role of Ca2+ in acid-sensing ion channel 1a-mediated chondrocyte pyroptosis in rat adjuvant arthritis
Wu X, Ren G, Zhou R, Ge J, Chen F. The role of Ca2+ in acid-sensing ion channel 1a-mediated chondrocyte pyroptosis in rat adjuvant arthritis. Laboratory Investigation 2018, 99: 499-513. PMID: 30487596, DOI: 10.1038/s41374-018-0135-3.Peer-Reviewed Original ResearchConceptsAcid-sensitive ion channelsChondrocyte pyroptosisCaspase-1Rheumatoid arthritisIL-18Role of Ca2Autoimmune diseasesIL-1βAdjuvant arthritis rat modelExpression of ASIC1aMain pathogenic featureRat adjuvant arthritisJoints of ratsArthritis rat modelApoptosis-associated speck-like proteinProinflammatory cytokines interleukinExpression of COL2ASpeck-like proteinASIC-1asAdjuvant arthritisPoor prognosisCytokines interleukinNLRP3 inflammasomeAA ratsRat model
2017
Effects of autophagy on acid-sensing ion channel 1a-mediated apoptosis in rat articular chondrocytes
Xie Y, Li Y, Zhou R, Dai B, Qian Y, Wu X, Ge J, Hu W, Chen F. Effects of autophagy on acid-sensing ion channel 1a-mediated apoptosis in rat articular chondrocytes. Molecular And Cellular Biochemistry 2017, 443: 181-191. PMID: 29273849, DOI: 10.1007/s11010-017-3223-6.Peer-Reviewed Original ResearchConceptsRat articular chondrocytesRheumatoid arthritisEffect of autophagyPathogenesis of RAArticular chondrocytesProgression of RAInhibition of ASIC1aAcid-sensing ion channel 1aMultiple pathogenic factorsDegenerative joint diseaseArticular chondrocyte apoptosisRat knee jointsIon channel 1aShort hairpin RNAPrecise etiologyJoint diseasePathogenic factorsKnee jointPsalmotoxin-1Chondrocyte apoptosisPrimary articular chondrocytesChannel 1aASIC1aAutophagy fluxBeclin-1ASIC2a overexpression enhances the protective effect of PcTx1 and APETx2 against acidosis-induced articular chondrocyte apoptosis and cytotoxicity
Zhou R, Ni W, Dai B, Wu X, Wang Z, Xie Y, Wang Z, Yang W, Ge J, Hu W, Chen F. ASIC2a overexpression enhances the protective effect of PcTx1 and APETx2 against acidosis-induced articular chondrocyte apoptosis and cytotoxicity. Gene 2017, 642: 230-240. PMID: 29141196, DOI: 10.1016/j.gene.2017.11.034.Peer-Reviewed Original ResearchMeSH KeywordsAcid Sensing Ion Channel BlockersAcid Sensing Ion ChannelsAcidosisAlkanesulfonic AcidsAnimalsApoptosisCells, CulturedChondrocytesCnidarian VenomsCollagen Type IIDrug SynergismGene Expression RegulationGenetic VectorsMAP Kinase Signaling SystemMorpholinesPeptidesPlasmidsRatsSpider VenomsConceptsAcid-sensing ion channel 1aArticular chondrocyte apoptosisChondrocyte apoptosisChondrocyte cytotoxicityRheumatoid arthritisProtective effectExpression of ASIC1aAcidosis-induced injuryERK1/2 MAPK signaling pathwaysIon channel 1aAlone groupNeuroprotective effectsChondroprotective effectsMAPK signaling pathwaysProtective roleType II collagenChannel 1aNeuronal cellsAPETx2ASIC2aPcTx1Phosphorylated ERK1/2Pathological charactersInduced apoptosisII collagenASIC1a Promotes Acid-Induced Autophagy in Rat Articular Chondrocytes through the AMPK/FoxO3a Pathway
Dai B, Zhu F, Chen Y, Zhou R, Wang Z, Xie Y, Wu X, Zu S, Li G, Ge J, Chen F. ASIC1a Promotes Acid-Induced Autophagy in Rat Articular Chondrocytes through the AMPK/FoxO3a Pathway. International Journal Of Molecular Sciences 2017, 18: 2125. PMID: 29019932, PMCID: PMC5666807, DOI: 10.3390/ijms18102125.Peer-Reviewed Original ResearchInterleukin-1β and tumor necrosis factor-α augment acidosis-induced rat articular chondrocyte apoptosis via nuclear factor-kappaB-dependent upregulation of ASIC1a channel
Zhou R, Dai B, Xie Y, Wu X, Wang Z, Li Y, Wang Z, Zu S, Ge J, Chen F. Interleukin-1β and tumor necrosis factor-α augment acidosis-induced rat articular chondrocyte apoptosis via nuclear factor-kappaB-dependent upregulation of ASIC1a channel. Biochimica Et Biophysica Acta (BBA) - Molecular Basis Of Disease 2017, 1864: 162-177. PMID: 28986307, DOI: 10.1016/j.bbadis.2017.10.004.Peer-Reviewed Original ResearchConceptsAcid-sensing ion channel 1aIL-1βRheumatoid arthritisInterleukin-1βNF-κB inhibitor ammonium pyrrolidinedithiocarbamateNF-κB DNA-binding activityPrimary articular chondrocytesProinflammatory cytokine interleukin-1βAcidosis-induced cytotoxicityCytokines interleukin-1βTumor necrosis factorImportant pathological characteristicNF-κB pathwayArticular chondrocyte apoptosisCaspase-3/9 expressionIon channel 1aNuclear factor-kappaBPleiotropic biological effectsArticular chondrocytesAgent BAPTA-AMASIC1a expressionChondrocyte cytotoxicityΚB expressionAdjuvant arthritisPathological characteristics
2015
Interleukin-6 enhances acid-induced apoptosis via upregulating acid-sensing ion channel 1a expression and function in rat articular chondrocytes
Zhou R, Wu X, Wang Z, Ge J, Chen F. Interleukin-6 enhances acid-induced apoptosis via upregulating acid-sensing ion channel 1a expression and function in rat articular chondrocytes. International Immunopharmacology 2015, 29: 748-760. PMID: 26359543, DOI: 10.1016/j.intimp.2015.08.044.Peer-Reviewed Original ResearchMeSH KeywordsAcid Sensing Ion ChannelsAcidsAnimalsAntibodies, Monoclonal, HumanizedApoptosisApoptosis Regulatory ProteinsArthritis, ExperimentalCartilage, ArticularChondrocytesDose-Response Relationship, DrugEnzyme ActivationInterleukin-6L-Lactate DehydrogenaseMalePeptidesRatsRats, Sprague-DawleySpider VenomsUp-RegulationConceptsInflammatory cytokine interleukin-6Acid-sensing ion channelsArticular chondrocyte apoptosisRat articular chondrocytesChondrocyte apoptosisRheumatoid arthritisAcid-induced cell injuryArticular chondrocytesAdjuvant arthritis ratsPrimary rat articular chondrocytesChronic inflammatory diseaseCytokine interleukin-6IL-6 receptorJAK2/STAT3Dose-dependent mannerArthritis ratsInflammatory diseasesInterleukin-6Specific antagonistCell injuryNF-κBAcid-induced apoptosisLDH releaseArthritic cartilageDownregulated expression