2022
Vaccination With Leptospira interrogans PF07598 Gene Family-Encoded Virulence Modifying Proteins Protects Mice From Severe Leptospirosis and Reduces Bacterial Load in the Liver and Kidney
Chaurasia R, Salovey A, Guo X, Desir G, Vinetz JM. Vaccination With Leptospira interrogans PF07598 Gene Family-Encoded Virulence Modifying Proteins Protects Mice From Severe Leptospirosis and Reduces Bacterial Load in the Liver and Kidney. Frontiers In Cellular And Infection Microbiology 2022, 12: 926994. PMID: 35837473, PMCID: PMC9274288, DOI: 10.3389/fcimb.2022.926994.Peer-Reviewed Original ResearchConceptsLethal challenge infectionChallenge infectionBacterial loadC3H/HeJ miceKey target organClinical pathogenesisSevere leptospirosisOrgan infectionProtein immunizationHeJ miceSerovar CanicolaTarget organsAnimal modelsLeptospirosis pathogenesisCellular pathogenesisMicePathogenesisInfectionLeptospira interrogansVirulence factorsModifying proteinsImmunizationKidneyLeptospirosisLiver
2020
Cytotoxicity of the 42 kDa SMase C sphingomyelinase secreted by Leptospira interrogans serovar Pomona on Vero cells.
Chaurasia R, Sritharan M. Cytotoxicity of the 42 kDa SMase C sphingomyelinase secreted by Leptospira interrogans serovar Pomona on Vero cells. Microbiology 2020, 166: 1065-1073. PMID: 32985970, DOI: 10.1099/mic.0.000976.Peer-Reviewed Original ResearchConceptsVero cellsSerovar PomonaHaemorrhagic manifestationsHost cell damageEarly diagnosisSevere formHuman patientsExperimental animalsLeptospira interrogansLeptospirosisCandidate markersSphingomyelinase activityHaemolytic activityPomonaSphingomyelinasePathogenic membersCytotoxicitySphingomyelinase geneActive formHigh levelsCellsPatientsDiagnosisConfocal imagesSphingomyelinases
2014
Highly immunoreactive antibodies against the rHup-F2 fragment (aa 63–161) of the iron-regulated HupB protein of Mycobacterium tuberculosis and its potential for the serodiagnosis of extrapulmonary and recurrent tuberculosis
Sritharan N, Choudhury M, Sivakolundu S, Chaurasia R, Chouhan N, Rao P, Sritharan M. Highly immunoreactive antibodies against the rHup-F2 fragment (aa 63–161) of the iron-regulated HupB protein of Mycobacterium tuberculosis and its potential for the serodiagnosis of extrapulmonary and recurrent tuberculosis. European Journal Of Clinical Microbiology & Infectious Diseases 2014, 34: 33-40. PMID: 25037869, DOI: 10.1007/s10096-014-2203-y.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayTB patientsExtrapulmonary tuberculosisPulmonary tuberculosisHealthy controlsMycobacterium tuberculosisExtrapulmonary TB patientsNormal healthy controlsRecurrent tuberculosisHousehold contactsSerum ironEnhanced immunoreactivityTuberculosisImmunoreactive antibodyAntigenic fragmentsPatientsImmunosorbent assayM. tuberculosisDiagnostic potentialSerumAntigenAntibodiesNegative correlationNegligible levelsHigh levels