2023
RBG Motif Bridge-Like Lipid Transport Proteins: Structure, Functions, and Open Questions
Hanna M, Guillén-Samander A, De Camilli P. RBG Motif Bridge-Like Lipid Transport Proteins: Structure, Functions, and Open Questions. Annual Review Of Cell And Developmental Biology 2023, 39: 409-434. PMID: 37406299, DOI: 10.1146/annurev-cellbio-120420-014634.Peer-Reviewed Original ResearchLipid transfer proteinMembrane contact sitesVesicle-mediated trafficTransport of lipidsPutative physiological roleEukaryotic cellsEndocytic pathwayContact sitesLipid transportPhysiological roleTransfer proteinProteinHydrophobic channelRod-like structureLipidsEntire lengthDevelopmental disordersCytosolMutationsNew familyTransportPathwayMechanismMembraneCells
2022
ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling
Hancock-Cerutti W, Wu Z, Xu P, Yadavalli N, Leonzino M, Tharkeshwar AK, Ferguson SM, Shadel GS, De Camilli P. ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling. Journal Of Cell Biology 2022, 221: e202106046. PMID: 35657605, PMCID: PMC9170524, DOI: 10.1083/jcb.202106046.Peer-Reviewed Original ResearchConceptsParkinson's diseasePD pathogenesisLeucine-rich repeat kinase 2 (LRRK2) G2019S mutationCGAS-STING pathwayAccumulation of lysosomesDNA-sensing cGAS-STING pathwayImmune activationLipid profileSTING signalingG2019S mutationAutosomal recessive Parkinson's diseaseRecessive Parkinson's diseaseModel human cell linesHuman cell linesCell linesPathogenesisLate endosomes/lysosomesDiseaseVPS13CEndosomes/lysosomesCurrent studyTransfer proteinActivationCellsPathwayMultimodal imaging of synaptic vesicles with a single probe
An SJ, Stagi M, Gould TJ, Wu Y, Mlodzianoski M, Rivera-Molina F, Toomre D, Strittmatter SM, De Camilli P, Bewersdorf J, Zenisek D. Multimodal imaging of synaptic vesicles with a single probe. Cell Reports Methods 2022, 2: 100199. PMID: 35497490, PMCID: PMC9046237, DOI: 10.1016/j.crmeth.2022.100199.Peer-Reviewed Original ResearchConceptsC2 domainSynaptic vesiclesSynaptic vesicle recyclingMembrane-binding C2 domainMultiple microscopy methodsEndocytic markersMembrane recyclingVesicle functionVesicle populationsCytosolic phospholipase ACell typesPhospholipase ADetectable tagMicroscopy modalitiesModular probesMultiple microscopy techniquesVesiclesComplete understandingDomainMicroscopy methodsMultiple levelsProbeAvailable probesMicroscopy techniquesPathwayPresynaptic autophagy is coupled to the synaptic vesicle cycle via ATG-9
Yang S, Park D, Manning L, Hill SE, Cao M, Xuan Z, Gonzalez I, Dong Y, Clark B, Shao L, Okeke I, Almoril-Porras A, Bai J, De Camilli P, Colón-Ramos DA. Presynaptic autophagy is coupled to the synaptic vesicle cycle via ATG-9. Neuron 2022, 110: 824-840.e10. PMID: 35065714, PMCID: PMC9017068, DOI: 10.1016/j.neuron.2021.12.031.Peer-Reviewed Original ResearchConceptsSynaptic vesicle cycleVesicle cyclePresynaptic autophagyAutophagosome biogenesisATG-9Only transmembrane proteinTrans-Golgi networkCellular degradation pathwayPresynaptic sitesActivity-dependent mannerTransmembrane proteinSynaptojanin 1Synaptic fociBiogenesisAutophagyNeuronal healthDegradation pathwayTraffickingPathwayParkinson's diseaseSynaptic activityNeuronal activityElegansSitesEndocytosis
2000
Epsin 1 Undergoes Nucleocytosolic Shuttling and Its Eps15 Interactor Nh2-Terminal Homology (Enth) Domain, Structurally Similar to Armadillo and Heat Repeats, Interacts with the Transcription Factor Promyelocytic Leukemia Zn2+ Finger Protein (Plzf)
Hyman J, Chen H, Di Fiore P, De Camilli P, Brunger A. Epsin 1 Undergoes Nucleocytosolic Shuttling and Its Eps15 Interactor Nh2-Terminal Homology (Enth) Domain, Structurally Similar to Armadillo and Heat Repeats, Interacts with the Transcription Factor Promyelocytic Leukemia Zn2+ Finger Protein (Plzf). Journal Of Cell Biology 2000, 149: 537-546. PMID: 10791968, PMCID: PMC2174850, DOI: 10.1083/jcb.149.3.537.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Vesicular TransportAmino Acid SequenceAnimalsArmadillo Domain ProteinsBeta CateninCalcium-Binding ProteinsCarrier ProteinsCell LineCell NucleusCrystallography, X-RayCytoskeletal ProteinsCytosolDNA-Binding ProteinsDrosophila ProteinsFluorescent Antibody TechniqueInsect ProteinsModels, MolecularMolecular Sequence DataNeuropeptidesPhosphoproteinsProtein BindingRatsSequence AlignmentTrans-ActivatorsTranscription FactorsVesicular Transport ProteinsZinc FingersConceptsENTH domainFinger proteinCRM1-dependent nuclear export pathwayClathrin adaptor AP-2Nuclear export pathwayAdaptor AP-2HEAT repeatsEndocytic machineryNuclear functionsHomology domainExport pathwayLeptomycin BEpsin 1AP-2Cytosolic proteinsUnknown functionDirect interactionEpsinTerminal portionClathrinProteinArmadillosAntifungal antibioticsPathwayDomain
1996
Alzheimer Amyloid Protein Precursor Is Localized in Nerve Terminal Preparations to Rab5-containing Vesicular Organelles Distinct from Those Implicated in the Synaptic Vesicle Pathway*
Ikin A, Annaert W, Takei K, De Camilli P, Jahn R, Greengard P, Buxbaum J. Alzheimer Amyloid Protein Precursor Is Localized in Nerve Terminal Preparations to Rab5-containing Vesicular Organelles Distinct from Those Implicated in the Synaptic Vesicle Pathway*. Journal Of Biological Chemistry 1996, 271: 31783-31786. PMID: 8943215, DOI: 10.1074/jbc.271.50.31783.Peer-Reviewed Original ResearchConceptsAmyloid protein precursorSmall synaptic vesiclesSynaptic vesiclesVesicular organellesProtein precursorSynaptic vesicle recycling pathwayNerve terminal preparationsSynaptic vesicle pathwayVesicle recycling pathwayAlzheimer amyloid protein precursorRecycling pathwayVesicle pathwayOrganellesVesiclesImmunoisolatesPathwayHomogeneous populationRab5Nerve terminalsHigh levelsDistinctPrecursorsSubstantial numberBilamellar vesicles
1995
Molecular mechanisms in synaptic vesicle recycling
De Camilli P. Molecular mechanisms in synaptic vesicle recycling. FEBS Letters 1995, 369: 3-12. PMID: 7641879, DOI: 10.1016/0014-5793(95)00739-v.Peer-Reviewed Original ResearchConceptsSynaptic vesicle recyclingVesicle recyclingSynaptic vesiclesMolecular mechanismsPowerful experimental modelVesicular trafficVesicle reformationNon-peptide neurotransmittersSecretory organellesRecycling pathwayHigh specializationVesiclesSpecialized formExocytosisRecent studiesNerve terminalsOrganellesMechanismRecyclingAbundanceCurrent informationPathwayUnique properties
1994
Formation of synaptic vesicles
Mundigl O, De Camilli P. Formation of synaptic vesicles. Current Opinion In Cell Biology 1994, 6: 561-567. PMID: 7986534, DOI: 10.1016/0955-0674(94)90077-9.Peer-Reviewed Original ResearchConceptsSynaptic vesiclesNew synaptic vesiclesSV membranesTransport vesiclesEvolutionary originNon-peptide neurotransmittersMembrane proteinsSecretory organellesFocal signalingVesiclesExocytosisMolecular compositionCellsGamma-aminobutyric acidOrganellesSignalingNerve cellsProteinDepolarization-induced Ca2PathwaySignificant progressNeurotransmittersMembraneAssemblyNerve terminalsRecycling and biogenesis of synaptic vesicles
McPherson P, De Camilli P. Recycling and biogenesis of synaptic vesicles. Seminars In Neuroscience 1994, 6: 137-147. DOI: 10.1006/smns.1994.1019.Peer-Reviewed Original ResearchSynaptic vesiclesRegulated secretory pathwaySpecialized organellesSecretory pathwayEarly endosomesConstitutive recyclingVesicular pathwayMembrane componentsVesiclesBiogenesisSecretory granulesDependent fashionPlasmalemmaPathwayEndosomesOrganellesExocytosisNervous systemRecyclingNerve terminalsAvailable informationOpen questionGranulesCa2